Utility of an internal control for evaluation of a Mycoplasma meleagridis PCR test.

Interlaboratory comparison of ability to detect nucleic acid ofMycoplasma gallisepticumandMycoplasma synoviaeby polymerase chain reaction

“…The PCR products obtained from the internal positive control can be easily distinguished from those obtained from the target sequences as they have different amplicon lengths. Therefore, false negative results due to inhibition of the PCR can be easily identified when using PCR in diagnostic investigations (Moalic et al , 1998;Carli & Eyigor, 2003). However, the presence of an internal positive control may interfere with amplification of the target DNA, resulting in false negative results (Sachadyn & Kur, 1998).…”

Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

“…Moreover, upon dilution the template prepared by above three methods, Simple broth dilution method were superior. The PK method was not much productive reported in earlier studies because it was unable to overcome the inhibitors in spite of diluting (1:100) the DNA preparation (Molaic et al, 1998). Alkaline lyses method was next easier method with higher PCR productivity.…”

“…There are many studies which have been carried out with direct clinical samples for mycoplasmas detection. During Cloacal swabs sampling used for M. melagridis detection, it has been found 40% inhibition of conventional PCR reaction in presence of Internal control (IC) in cocktail (Molaic et al, 1998). Even Taqman real time PCR fidelity were compromised due to inhibitors carried with clinical samples in spite of DNA were extracted with commercial kits (Sprygin et al, 2010).…”

“…Direct PCR from clinical specimen produces false negative results due to undefined inhibitory substance (Molaic et al, 1998); false positive due to contamination during processing clinical samples (Ma, 1995). Alternatively, culture-enhanced PCR (CE-PCR) was introduced by Abele-Horn et al (1998).…”

Infection of the turkey embryonic trachea withMycoplasma meleagridis

“…In mature turkeys, however, the organism is localized in the genital tract (Yamamoto, 1991). Recently, an increasing amount of attention has been paid to the trachea (Moalic et al, 1997(Moalic et al, , 1998 because using tracheal swabs on turkeys with respiratory signs permits the isolation of M. meleagridis and the amplification of M. meleagridis DNA sequences by polymerase chain reactions. This recent trend indicates the increasing importance of the upper respiratory tract in the transmission of M. meleagridis.…”