Utility of a modified silver staining technique for detection of Leptospira

Gunasekara, T.D.C.P.; Sumaiha, M. H. F.; Damayanthi, M. K. S.; Weerasekera, Manjula; Fernando, S.S.N.

doi:10.4038/sljid.v7i2.8142

Cited by 2 publications

(4 citation statements)

References 8 publications

(11 reference statements)

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“…Here, Leptospires were detected in urine smears stained by Fontana Method as long, thin, brownish black spirochaetes against yellow background. This is in agreement with previous studies by (Rodríguez et al, 2013;Gunasekara et al, 2017).…”

Section: Discussionsupporting

confidence: 94%

“…These results indicated that, the Fontana Method is an easy, rapid and cost effective test for detection of Leptospires in cattle and dogs' urine, which can be used in laboratories, where DFM is unavailable (Yesilmen et al, 2012;Gunasekara et al, 2017). Fontana Method has higher sensitivity when compared with DFM (Rodríguez et al, 2013).…”

Section: Discussionmentioning

confidence: 95%

“…Silver Impregnation Method "Fontana Method" is used for staining smears by deposition of silver on Leptospira, which leads to increase its thickness, and then Leptospira can be demonstrated by light microscopy as brownish black organisms against yellow background (Gunasekara et al, 2017;Lather et al, 2009;Rodríguez et al, 2013). Fontana Method is a relatively inexpensive method, the stained bacteria can be observed by using the ordinary light microscope, which is available in a basic laboratory setting (Gunasekara et al, 2017). However, Fontana Method may have some drawbacks such as lack of sensitivity with a high risk for false-positive and false-negative results (Klaasen and Adler, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Leptospira in cattle and dogs in Dakahlia Governorate

Towheed

Atwa

Eisa

et al. 2019

Benha Veterinary Medical Journal

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The study was applied on a total of 115 urine samples, collected from suspected cattle (97) and their in-contact dogs (18), which suffered from different clinical signs of leptospirosis. The collected samples were examined for Leptospira spp. by different methods as Dark Field Microscopy (DFM) and staining by Silver Impregnation Method "Fontana Method". The results of DFM showed that, 28 out of 97 (28.9%) cattle urine samples were positive, and eight out of 18 (44.4%) dogs' urine samples were positive. Additionally, the results of Fontana Method revealed that, 44 out of 97 (45.4%) cattle urine samples were positive, and 13 out of 18 (72.2%) dogs' urine samples were positive. To confirm these results, conventional polymerase chain reaction (PCR) targeting (23S rDNA) gene of Leptospira, was done on 6 urine samples, included 3 cattle urine samples and 3 dogs' urine samples (one sample was positive, and two samples were negative in each species). PCR result confirmed the DFM and Fontana method results. In addition, a treatment trial for three groups of cattle infected with leptospirosis was performed by Dihydrostreptomycin, Oxytetracycline and Amoxicillin, respectively for 3 days. This study showed higher prevalence of leptospirosis in Dakahlia by DFM and Fontana methods. Moreover, Dihydrostreptomycin, Oxytetracycline and Amoxicillin have been successfully affecting on leptospirosis.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Detection of Leptospira in cattle and dogs in Dakahlia Governorate

Towheed

Atwa

Eisa

et al. 2019

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

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“…A variety of histopathological stains are used for the detection of leptospires in clinical specimens. Although silver stains have been documented to be of use in the literature [ 212 ], the Warthin-Starry stain is widely in use now. Azizi et al [ 213 ] commented that histopathological stains (including Warthin-Starry stain) can sometimes confer false negative results, as the leptospirosis burden in tissue biopsies (such as kidneys) may not be significant.…”

Section: Diagnosis Of Leptospirosismentioning

confidence: 99%

Leptospiral Infection, Pathogenesis and Its Diagnosis—A Review

et al. 2021

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Leptospirosis is a perplexing conundrum for many. In the existing literature, the pathophysiological mechanisms pertaining to leptospirosis is still not understood in full. Considered as a neglected tropical zoonotic disease, leptospirosis is culminating as a serious problem worldwide, seemingly existing as co-infections with various other unrelated diseases, including dengue and malaria. Misdiagnosis is also common as non-specific symptoms are documented extensively in the literature. This can easily lead to death, as the severe form of leptospirosis (Weil’s disease) manifests as a complex of systemic complications, especially renal failure. The virulence of Leptospira sp. is usually attributed to the outer membrane proteins, including LipL32. With an armament of virulence factors at their disposal, their ability to easily adhere, invade and replicate within cells calls for a swift refinement in research progress to establish their exact pathophysiological framework. As an effort to reconstitute the current knowledge on leptospirosis, the basis of leptospiral infection, including its risk factors, classification, morphology, transmission, pathogenesis, co-infections and clinical manifestations are highlighted in this review. The various diagnostic techniques are also outlined with emphasis on their respective pros and cons.

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Utility of a modified silver staining technique for detection of Leptospira

Cited by 2 publications

References 8 publications

Detection of Leptospira in cattle and dogs in Dakahlia Governorate

Detection of Leptospira in cattle and dogs in Dakahlia Governorate

Leptospiral Infection, Pathogenesis and Its Diagnosis—A Review

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