Utility of 2-D and 3-D Virtual Microscopy in Cervical Cytology Education and Testing

Dee, Fred R.; Donnelly, Amber; Radio, Stanley J.; Leaven, Timothy; Zaleski, M. Sue; Kreiter, Clarence D.

doi:10.1159/000325788

Cited by 41 publications

(57 citation statements)

References 18 publications

(14 reference statements)

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“…In WSI, the field is fixed to 1 focus plane, and it is not possible to focus up and down as in conventional microscopes. Although new technology on 3-dimensional WSI is available and able to increase the focus planes on digital images, the impact on diagnostic accuracy seems to be minimal [9]. Indeed, the majority of the participants were unfamiliar with WSI (86.11%) and thought that the conventional microscope was superior to WSI in diagnostic quality (72.22%) in our posttest feedback survey.…”

Section: Discussionmentioning

confidence: 97%

Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan

et al. 2015

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Objective: In a peer comparison educational program, transferring glass slides between laboratories and collecting responses are time- and cost-consuming. Integrating a web-based whole-slide imaging (WSI) system and online questionnaires may serve as a promising solution. Study Design: Five gynecologic Papanicolaou-stained smears and 5 nongynecologic slides were selected. The 10 whole-slide images were acquired by a Leica SCN-400 system and released via an Aperio eSlide Manager. Online questionnaires generated by Google Forms with access to the 10 whole-slide images were released to all the practitioners in Taiwan by e-mail. After closing the program, an online posttest feedback survey was conducted. Results: A total of 302 participants joined the gynecologic test, and 291 joined the nongynecologic test. The correct interpretation rates were 81.8-93.7% in the former and 28.5-93.1% in the latter. In the posttest feedback survey, there were 63.2% of the participants reporting first-time WSI experience, and 97.9% of them said they would like to participate in a similar program again. Conclusion: Integrating a web-based WSI system and online questionnaires is an easy method to access nationwide practitioners. Participants can make interpretations using WSI even without prior experience. The model is valuable for those who want to initiate a large-scale cytopathology peer comparison educational program.

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Section: Discussionmentioning

confidence: 97%

Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan

et al. 2015

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“…[9][10][11][12][13] Some WSI scanners are capable of composing a three-dimension-like image by using multifocus frame images, and others can create a multilayer scanning image, which, for example, are stacked 10 layers each 2 lm apart along the z-axis. 13 Indeed, Dee et al 12 have reported the three-dimensional WSI system was useful for the education and testing of cervical cytology. However, will we use the system for the cytodiagnosis or consultation of routine cases?…”

Section: Discussionmentioning

confidence: 99%

Comparison between a traditional single still image and a multiframe video image along the z‐axis of the same microscopic field of interest in cytology: Which does contribute to telecytology?

Yamashiro¹,

Taira²,

Matsubayashi³

et al. 2009

Diagnostic Cytopathology

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The limitation of cytologic still images is one of the reasons why telecytology has not met with widespread acceptance by the cytology community. Cytologic still image only displays a single depth of field, and this is a particularly acute problem in cytology where the specimen is often much thicker than a single microscopic depth of focus. In this article, we examine the validity of a "z-axis" video of a microscopic field of interest.After observing videos of fields of interest from 10 cases, five cytotechnologists reached suitable cytologic findings and diagnosed the fields correctly in great majority of cases. Five other cytotechnologists, who looked only at a single representative still image, could not always make a correct diagnosis. The difference between two observer groups was statistically significant by Wilcoxon's matched pairs signed-rank test.The results indicate that "z-axis" video of microscopic field of interest provides a similar experience to "focusing through" observation of the specimen under a microscope and may improve an accuracy of primary telecytodiagnosis. And we expect that video image telecytology will strongly influence cytology, especially in education and training.

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“…3). Interestingly, a study aimed at defining differences in performance of observers using 2-dimensional versus 3-dimensional cytology images showed no difference in diagnostic accuracy; however, it did highlight a higher level of ‘uncertainty and frustration’ of the observers who were unable to resolve out-of-focus cells on the 2-dimensional whole-slide images [4]. At present, a major limiting factor in the use of whole-slide images is the high cost of the scanning devices.…”

Section: Methods Of Digital Pathology Image Transfermentioning

confidence: 99%

Digital Cytology: Current State of the Art and Prospects for the Future

Wilbur¹

2011

Acta Cytologica

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The growth of digital methods in pathology is accelerating. Digital images can be used for a variety of applications in cytology, including rapid interpretations, primary diagnosis and second opinions, continuing education and proficiency testing. All of these functions can be performed using small static digital images, real-time dynamic digital microscopy, or whole-slide images. This review will discuss the general principles of digital pathology, its methods and applications to cytologic specimens. As cytologic specimens have unique features compared to histopathology specimens, the key differences will be discussed. Technical and administrative issues in digital pathology applications and the outlook for the future of the field will be presented.

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Utility of 2-D and 3-D Virtual Microscopy in Cervical Cytology Education and Testing

Cited by 41 publications

References 18 publications

Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan

Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan

Comparison between a traditional single still image and a multiframe video image along the z‐axis of the same microscopic field of interest in cytology: Which does contribute to telecytology?

Digital Cytology: Current State of the Art and Prospects for the Future

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