Utility and pattern of positivity of p40 in the diagnosis of squamous cell carcinoma of the lung by cytology: the first study on fine needle aspiration smears

Jain, Deepali; Mathur, Sandeep; Guleria, Randeep; Iyer, V. K.

doi:10.1111/cyt.12105

Cited by 18 publications

(13 citation statements)

References 19 publications

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“…Cytology specimens are of paramount importance, not only in the diagnosis of tumors but for performing ancillary studies successfully [10][11][12] . Several recent publications regarding the use of cytological specimens in molecular testing of lung cancer have been published [3, 5-7, 13, 14] .…”

Section: Discussionmentioning

confidence: 99%

Use of Exfoliative Specimens and Fine-Needle Aspiration Smears for Mutation Testing in Lung Adenocarcinoma

Jain¹,

Ramachandrappa²,

Singh³

et al. 2017

Acta Cytologica

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Objective: Cytology specimens are considered to be a promising alternative for detecting driver mutations in lung cancer patients. We aimed to explore the suitability and utility of various cytology samples of non-small-cell lung cancer (NSCLC) patients for mutation testing. In addition to mutation detection, the importance of preanalytic factors was discussed. Design: A total of 116 cytology samples including 32 controls comprising pleural effusions, bronchial washings, and direct fine-needle aspiration (FNA) smears were included in the study for the detection of EGFR, KRAS, and Her-2/neu gene mutations. Tumor content was checked by microscopic evaluation. Tumor enrichment was done by scraping direct smears. DNA yield was assessed before selecting the method of mutation detection. Sanger sequencing and real-time PCR-based methods were used. Results: Overall, 20.23% EGFR mutations and 2.74% KRAS mutations were observed in this study. Nondriver genetic polymorphisms were observed in EGFR exon 20 in 37% cases. The coexistence of the EGFR mutation and EGFR polymorphism was seen in 7 cases. DNA yield was better in pleural effusions and bronchial washings. Real-time PCR was used in specimens of low DNA yield. Conclusions: Cytology samples are useful in ascertaining molecular diagnostic information for treatment purposes if they are optimized judiciously in their preanalytic phase.

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Section: Discussionmentioning

confidence: 99%

Use of Exfoliative Specimens and Fine-Needle Aspiration Smears for Mutation Testing in Lung Adenocarcinoma

Jain¹,

Ramachandrappa²,

Singh³

et al. 2017

Acta Cytologica

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“…p40 expression has been reported to be specific for squamous cell carcinoma of the head and neck, esophagus, thymomas, urothelial carcinomas and lung squamous cell carcinoma [53,55,56,57,58]. Immunocytochemistry for p40 has been applied to cytology samples, such as cell blocks and fine-needle aspirates [59,60,61], with Collins et al [49] describing p40 as having 89% sensitivity and 100% specificity for non-small cell lung carcinoma. In our study, the sensitivity and specificity for p40 in squamous cell carcinoma were 84 and 98%, respectively (table 3).…”

Section: Discussionmentioning

confidence: 99%

Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique

Kawai

Tominaga²,

Hiroi³

et al. 2015

Acta Cytologica

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Background: The introduction of new therapies has made it important to differentiate between adenocarcinoma and squamous cell carcinoma. To allow the use of various immunocytochemical stains on limited materials, we tried transferring cells from a given smear to multiple slides. Using touch-preparation samples of 215 surgically resected non-small cell lung carcinomas of confirmed histologic classification (adenocarcinoma,n = 101; squamous cell carcinoma,n = 114), we performed immunocytochemistry for thyroid transcription factor-1, napsin A, p40, p63, CK5/6 and desmocollin-3, and compared cytologic staining results with the corresponding resection. Methods: We examined: (a) the expressions of the above 6 antibodies on cells transferred from touch imprints of resected specimens, the extent of staining being considered positive if more than 5% of the area was stained, and (b) the sensitivity, specificity, positive predictive value and negative predictive value for each antibody. Results: The histologic corresponding rate with Papanicolaou staining was only 73%. Regarding the differentiation of adenocarcinoma from squamous cell carcinoma, the sensitivity and specificity for napsin A in adenocarcinoma were 80 and 97%, respectively, while those for p40 in squamous cell carcinoma were 84 and 98%, respectively. Conclusion: The immunocytochemical expressions of napsin A and p40 in imprint cytology seem to be of great utility for the accurate histological differentiation of lung cancers.

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“…Although the World Health Organisation does recommend pattern subtyping on small biopsies and cytology samples whenever possible, its utility is controversial due to the sampling bias that will likely lead to incorrect classification. Nevertheless, subtyping of non‐small cell lung carcinomas (NSCLC) into ADs and squamous cell carcinomas has been found to be accurate and definite on cytology and small biopsy specimens . Among ADs alone, one previous study showed that cytological features correlate with prognosis, while others found that pattern grading on small biopsies predicts response to chemotherapy .…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, subtyping of non-small cell lung carcinomas (NSCLC) into ADs and squamous cell carcinomas has been found to be accurate and definite on cytology and small biopsy specimens. 7,8 Among ADs alone, one previous study showed that cytological features correlate with prognosis, 9 while others found that pattern grading on small biopsies predicts response to chemotherapy. [10][11][12][13] In addition, knowledge of the varied patterns of lung ADs and its recognition will aid in triage of material for molecular analysis.…”

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confidence: 99%

Adenocarcinoma predominant pattern subtyping and nuclear grading in cytology: Is there a role in prognostication of advanced pulmonary adenocarcinomas?

et al. 2018

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Utility and pattern of positivity of p40 in the diagnosis of squamous cell carcinoma of the lung by cytology: the first study on fine needle aspiration smears

Cited by 18 publications

References 19 publications

Use of Exfoliative Specimens and Fine-Needle Aspiration Smears for Mutation Testing in Lung Adenocarcinoma

Use of Exfoliative Specimens and Fine-Needle Aspiration Smears for Mutation Testing in Lung Adenocarcinoma

Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique

Adenocarcinoma predominant pattern subtyping and nuclear grading in cytology: Is there a role in prognostication of advanced pulmonary adenocarcinomas?

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