Utilising a novel surveillance system to enhance field screening activities for the leishmaniases

Panahi, Elina; Shivas, Martin A.; Hall‐Mendelin, Sonja; Kurucz, Nina; Rudd, Penny A.; Araujo, Rachel De; Skinner, Eloise B; Melville, Lorna; Herrero, Lara J.

doi:10.1016/j.mex.2020.101156

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…By using cellulose filter paper impregnated with various chemicals to help lyse cellular membranes from samples, the released DNA can be protected from degradation for up to 2 years. More recently, impregnated cellulose paper has been developed for molecular testing of various pathogens, including SARS-CoV-2, leishmaniasis, and malaria [47][48][49]. The HIV in separated plasma is directly lysed, and viral nucleic acids are enriched by an integrated, flow-through FTA ® membrane in the concentrator, which enables nucleic acid preparation with high efficiency [50] (Fig.…”

Section: Porous Materials-based Strategiesmentioning

confidence: 99%

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Wang

Hong

et al. 2022

Military Med Res

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Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a highly miniaturized, automatic, and integrated technology, are a potential substitute for traditional methods in performing rapid, low-cost, accurate, and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First, we introduce the typical on-chip nucleic acid processes, including sample preprocessing, amplification, and signal read-out. Then, four categories of microfluidic platforms are compared with respect to features, merits, and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally, we propose future directions for microfluidics-based infectious disease diagnosis.

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Section: Porous Materials-based Strategiesmentioning

confidence: 99%

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Wang

Hong

et al. 2022

Military Med Res

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“…aegypti [49], Ae. albopictus [50], Culex species [51] and Forcipomyia (Lasiohelea) [52]. To date, the feasibility of using FTA cards for detection of triatomine bugs or T. cruzi has not been assessed.…”

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confidence: 99%

Towards environmental detection of Chagas disease vectors and pathogen

Gysin

Urbano

Brandner-Garrod

et al. 2021

Preprint

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Background: Accurate surveillance of triatomine household infestation is crucial for Chagas disease vector control. However, no gold standard detection method with high levels of sensitivity or specificity is currently available. Several intrinsic features of triatomine bug behaviour and the lifecycle of Trypanosoma (T.) cruzi lead to deposition of environmental DNA (eDNA) in infested houses. This study evaluated the use of FTA cards and cotton-tipped swabs as low-technology, cost-effective tools for simultaneous detection of T. cruzi and vector eDNA in the laboratory and field. Methods/Principal Findings: This study had two components: (1) laboratory evaluation and optimisation of QIAcard® FTA® classic cards to detect Rhodnius (R.) prolixus eDNA by altering five different environmental variables (darkness, triatomine number, temperature, feeding status and degradation at ambient temperature); (2) detection of R. prolixus and T. cruzi eDNA from cotton-tipped house wall swabs from an endemic region in Casanare Department, Colombia. eDNA was extracted from all specimens and amplified using a multiplex TaqMan qPCR assay targeting the R. prolixus 12S rRNA gene and T. cruzi satellite DNA region. R. prolixus eDNA from five 3rd/4th instar nymphs was successfully amplified from FTA cards after as little as 15 minutes of contact time under standard insectary conditions. Factors significantly increasing eDNA detection from FTA cards were increasing temperature from 21oC to 27-32oC, triatomine bug density from 1-25 bugs and recent blood-feeding. eDNA was detectable from FTA cards stored at room temperature for at least two weeks. In cotton-tipped swabs from the field, the sensitivity and specificity of R. prolixus eDNA detection was 60.6% (n=20/33) and 100% (n=33/33), respectively. T. cruzi eDNA was amplified from 93.9% (n=31/33) of infested houses. Conclusions/Significance: FTA cards are a highly sensitive tool for entomological surveillance of R. prolixus and exhibit little variability under different environmental conditions. Additionally, cotton-tipped swabs are a relatively sensitive tool for entomological and parasitological surveillance of R. prolixus and T. cruzi in situ, but more feasible due to low cost. Both methods could be utilised by citizen science initiatives to contribute to the control of Chagas disease in endemic communities.

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Utilising a novel surveillance system to enhance field screening activities for the leishmaniases

Cited by 2 publications

References 4 publications

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Microfluidics-based strategies for molecular diagnostics of infectious diseases

Towards environmental detection of Chagas disease vectors and pathogen

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