Utilidad de la citología de esputo inducido en el estudio de masas centrales en ancianos

Fernández, A; Río, F. García; Alises, Sagrario Mayoralas; Fraga, S. Sánchez; Andrés, Olga Mediano San; León, J. Villamor

doi:10.1016/s0014-2565(01)70876-4

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…[13] IS also helpful in diagnosis of pneumocystis pneumonia,[14] eosinophilic asthma,[15] pleural TB,[16] interstitial lung disease,[17] and lung cancer. [18]…”

Section: Discussionmentioning

confidence: 99%

Induced sputum versus bronchial washings in the diagnosis of sputum negative pulmonary tuberculosis

Rao

Mandava

Rani

et al. 2016

J Family Med Prim Care

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Introduction:Tuberculosis (TB) is one of the most important public health problems worldwide. Detecting patients with active pulmonary TB (PT) is an important component of TB control programs. However, at times in patients even with a compatible clinical picture, sputum smears do not reveal acid-fast bacilli (AFB) and smear-negative PT remains a common problem. This study compares the results of induced sputum (IS) and bronchial washings (BWs) in detecting sputum negative PT.Materials and Methods:A prospective study conducted from June 2014 to June 2015, comprising 120 patients fulfilling study criteria. Patients with respiratory symptoms and chest roentgenogram suspicious of PT with no previous history of antiTB treatment (ATT) and two spontaneous sputum smear samples negative for AFB were included in the study. Patients with active hemoptysis and sputum positive were excluded from the study. Sputum induction was done using 5–10 ml of 3% hypertonic saline through ultrasonic nebulizer taking safety precautions. All the patients underwent fiberoptic bronchoscopy after 6 h fasting on the same day. About 20 ml of normal saline instilled into the suspected pathology area and washings were taken with gentle suction. The sample processing and fluorescent staining for AFB were done in a designated microscopy laboratory.Results:Of 120 smear-negative PT patients, IS smear examination detected AFB in 76 patients (63.3%) and AFB detected from BWs in 94 patients (78.5%). Smear positivity higher in cavitary and infiltrative lesions compared to consolidation and infrahilar pattern disease.Conclusions:Even though both IS and BWs procedures were valuable for the diagnosis of smear-negative TB, sputum induction with hypertonic saline should be the initial procedure of choice, which can be repeated twice/thrice in a day or 2 consecutive days. If the patient remains IS smear-negative and if the clinical probability of TB is high, starting ATT and closely monitoring patient and reserving bronchoscopy to those patients who do not improve and to exclude alternative diagnosis seems to be a practically useful approach.

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“…[13] IS also helpful in diagnosis of pneumocystis pneumonia,[14] eosinophilic asthma,[15] pleural TB,[16] interstitial lung disease,[17] and lung cancer. [18]…”

Section: Discussionmentioning

confidence: 99%

Induced sputum versus bronchial washings in the diagnosis of sputum negative pulmonary tuberculosis

Rao

Mandava

Rani

et al. 2016

J Family Med Prim Care

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“…Kirsch et al (38) showed that the analysis of induced sputum can be used in diagnosing Pneumocystis carinii (currently named Pneumocystis jirovecii) pneumonia (38, 39). In addition, induced sputum can be used as a diagnostic tool in detecting pulmonary tuberculosis (40)(41)(42)(43), pulmonary sarcoidosis (44, 45), and lung cancer (46)(47)(48)(49). Therefore, the introduction of this method in pathological laboratories will provide an additional tool for diagnosing different pulmonary diseases (53).…”

Section: Applications Of Induced Sputummentioning

confidence: 99%

Sputum induction and its diagnostic applications in inflammatory airway disorders: a review

Goncalves,

Eze

2023

Front. Allergy

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Sputum induction is a technique that covers the induction and the subsequent processing of the expectorate primarily for the analysis of cells and different inflammatory biomarkers present in the airways to further understand the pathophysiology of different inflammatory respiratory disorders such as asthma and chronic obstructive pulmonary disease (COPD) as well as the diagnosis of lung diseases such as lung cancer, tuberculosis, and Pneumocystis jirovecii pneumonia. It is a non-invasive, safe, cost-effective, and reliable technique reported to exhibit a high success rate. However, due to being technically demanding and time-consuming and having the need of employing trained staff, this technique is only used in restricted research centres and in limited centres of clinical use. When the sputum is collected after induction, the primary goal is to obtain a differential cell count and evaluate the molecular biomarkers of airway inflammation such as eosinophil cationic protein, eosinophil-derived neurotoxin, major basic protein, tryptase, cytokine production [e.g., interleukin (IL)-5], albumin, and fibrinogen. In addition, cytospins from the processed sputum are used for immunocytochemical staining of cellular products such as EG-2 reactive protein, granulocyte-macrophage colony-stimulating factor, tumour necrosis factor alpha, and IL-8 that play significant roles in understanding the pathophysiology of inflammatory airway diseases. Nowadays, this technique can be further used by performing an additional analysis such as flow cytometry and in situ hybridisation on the sputum supernatant to investigate more the immune response and pathophysiological process of such various respiratory diseases. In addition, the application of sputum fluid phase to assess the biomarkers could be used more routinely in pathological laboratories for diagnosing lung cancer, COPD, and asthma as well as for monitoring lung cancer progression and asthma and COPD treatment, allowing for early detection and a better treatment provided by the clinicians.

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Induced sputum analysis: step by step

Weiszhár

Horváth

2013

Breathe

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[ To introduce the standardised method for sputum induction [ To provide a guide for sputum processing [ To highlight important methodological aspects that may influence results [ To briefly summarise potential clinical and research use SummarySputum induction is a relatively non-invasive mode of airway sampling that provides an opportunity for analysis of cellular components and infective agents, including bacteria and viruses, together with fluid-phase constituents. Both induction and processing of sputum samples are standardised and several manuals are available to help to educate professionals to perform the technique to the highest standard [36]. Using this standardised technique, the results are reproducible and comparable between different laboratories. Detailed studies on methodological issues provided enough evidence for standardisation and careful use of the methodology enabled the field to develop rapidly from a research tool to a useful clinical test. Eosinophil differential count in induced sputum is a recommended, evidence-based mode of assessing airway inflammation in asthma and its use is incorporated into the relevant guidelines.

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Utilidad de la citología de esputo inducido en el estudio de masas centrales en ancianos

Cited by 4 publications

References 14 publications

Induced sputum versus bronchial washings in the diagnosis of sputum negative pulmonary tuberculosis

Induced sputum versus bronchial washings in the diagnosis of sputum negative pulmonary tuberculosis

Sputum induction and its diagnostic applications in inflammatory airway disorders: a review

Induced sputum analysis: step by step

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