Utilidad de la biología molecular en el diagnóstico microbiológico de las infecciones por micobacterias

Domínguez, José; Blanco, Silvia; Lacoma, Alícia; García-Sierra, Nerea; Prat, Cristina; Ausina, V.

doi:10.1016/s0213-005x(08)76539-x

Cited by 10 publications

(5 citation statements)

References 49 publications

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“…Molecular tests may show discordant results when compared to phenotypic DST based on critical concentration testing. 74,98,[105][106][107][108][109][110][111][112] The clinical consequences of the limitations in the accuracy of molecular resistance assays depend on the drug, but follow a general pattern. False-positive test results will lead to drug-susceptible TB being treated with one or more second-line drugs, i.e., treatment that is generally more toxic, less effective, prolonged and more expensive.…”

Section: Relationship Between the Results Of Genotypic And Phenotypicmentioning

confidence: 99%

Clinical implications of molecular drug resistance testing for <I>Mycobacterium tuberculosis</I>: a TBNET/RESIST-TB consensus statement

Domínguez

Boettger

Cirillo

et al. 2016

int j tuberc lung dis

122

105

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Section: Relationship Between the Results Of Genotypic And Phenotypicmentioning

confidence: 99%

Clinical implications of molecular drug resistance testing for <I>Mycobacterium tuberculosis</I>: a TBNET/RESIST-TB consensus statement

Domínguez

Boettger

Cirillo

et al. 2016

int j tuberc lung dis

122

105

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“…A large number of Mycobacterium-Escherichia coli shuttle vectors have been developed for the transfer of foreign genes into mycobacteria [ 26 ]. These shuttle vectors are maintained in mycobacteria either episomally or through integration into the mycobacterial genome.…”

Section: Introductionmentioning

confidence: 99%

The Development of a Novel Mycobacterium-Escherichia coli Shuttle Vector System Using pMyong2, a Linear Plasmid from Mycobacterium yongonense DSM 45126T

Lee

Kim

et al. 2015

PLoS ONE

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The Mycobacterium-Escherichia coli shuttle vector system, equipped with the pAL5000 replicon, is widely used for heterologous gene expression and gene delivery in mycobacteria. Despite its extensive use, this system has certain limitations, which has led to the development of alternative mycobacterial vector systems. The present study describes the molecular structure and expression profiles of a novel 18-kb linear plasmid, pMyong2, from Mycobacterium yongonense. Sixteen open reading frames and a putative origin of replication were identified, and the compatibility of the pMyong2 and pAL5000 vector systems was demonstrated. In recombinant Mycobacterium smegmatis (rSmeg), the pMyong2 vector system showed a copy number that was approximately 37 times greater than that of pAL5000. Furthermore, pMyong2 increased the mRNA and protein expression of the human macrophage migration inhibitory factor (hMIF) over pAL5000 levels by approximately 10-fold and 50-fold, respectively, demonstrating the potential utility of the pMyong2 vector system in heterologous gene expression in mycobacteria. Successful delivery of the EGFP gene into mammalian cells via rSmeg carrying the pMyong2 vector system was also observed, demonstrating the feasibility of this system for DNA delivery. In conclusion, the pMyong2 vector system could be effectively used not only for the in vivo delivery of recombinant protein and DNA but also for mycobacterial genetic studies as an alternative or a complement to the pAL5000 vector system.

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“…Molecular biology methods are always based on strong clinical suspicion and histopathological findings, thereby offering a promising scenario for extra-pulmonary TB diagnosis (21,22) ♣…”

Section: Discussionmentioning

confidence: 99%

The molecular diagnosis of pelvic tuberculosis: a case report

Faccini-Martínez¹,

Espinal²,

Jojoa³

et al. 2012

Rev. salud pública

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Tuberculosis is a re-emerging infectious disease. A retrospective analysis was made of the clinical history of a 48-year-old woman in April 2009; she was a secretary at a third-level hospital living in an urban area. Pelvic tubercular infection was suggested as a possible diagnosis; spoligotyping molecular methodology was used on a peritoneal secretion sample to confirm such diagnosis and confirmed the presence of Mycobacterium tuberculosis (octal code 777777777760771, SIT 53, Family T1).

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Utilidad de la biología molecular en el diagnóstico microbiológico de las infecciones por micobacterias

Cited by 10 publications

References 49 publications

Clinical implications of molecular drug resistance testing for <I>Mycobacterium tuberculosis</I>: a TBNET/RESIST-TB consensus statement

Clinical implications of molecular drug resistance testing for <I>Mycobacterium tuberculosis</I>: a TBNET/RESIST-TB consensus statement

The Development of a Novel Mycobacterium-Escherichia coli Shuttle Vector System Using pMyong2, a Linear Plasmid from Mycobacterium yongonense DSM 45126T

The molecular diagnosis of pelvic tuberculosis: a case report

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