Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea Acida in Wistar Rats: An in Vitro Study.

Abstract

Background: Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to solve difficult childbirth. This study aimed to evaluate the in vitro uterotonic effects of aqueous and methanolic extracts of L. acida in female Wistar rats. Uterine strips isolated from female rats pretreated (48h) with oestradiol (5μg) were mounted in a single-organ bath containing a well aerated and thermostated De Jalon solution (37°C). The effects of L. acida extracts were recorded in a non-cumulative manner after application. The effect of the methanolic extract (the most active extract) was monitored in the presence of atosiban (a competitive antagonist of oxytocin receptors), atropine (a specific type 3 muscarinic receptor antagonist), nifedipine (an L-type calcium channel antagonist) and 2-Aminoethoxydiphenyl borate (2-ADB, a specific antagonist of inositol 1,4,5-triphosphate receptors type 1), and in calcium-free medium containing EGTA. Results: L. acidainduced uterine contraction in a concentration-dependent manner with the methanolic extract (1.506 ± 0.032 gf) being the most effective. Administration of atosiban (2 μmol/l), atropine (1 μmol/l), nifedipine (5 μmol), 2-APB (100 μmol), and calcium free medium containing EGTA (2 mmol) reduced the contractile effect of L. acida. Complete inhibition was observed with nifedipine, 2-APB, and calcium free medium containing EGTA.Conclusions: These results suggest that L. acida possesses an uterotonic effect mediated through oxytocin receptors with mobilization of extracellular calcium.

Based on UPLC/MS/MS and Bioinformatics Analysis to Explore the Difference Substances and Mechanism of Curcumae Radix (Curcuma wenyujin) in Dysmenorrhea

“…Studies have shown that the expression of oxytocin receptor increases significantly with the increase of dysmenorrhea. [39] After stimulation with oxytocin, the contractile tension of uterine smooth muscle increased significantly (p < 0.05, p < 0.01), which was consistent with that reported in the literature, [40] suggesting that oxytocin can induce strong contraction similar to dysmenorrhea. Further studies showed that CW extract could significantly inhibit oxytocin-induced uterine spasm in vitro, and significantly reduce the contraction amplitude, frequency, and activity of uterine smooth muscle.…”

"Ethanol Extract of Lannea Acida A. Rich (Anacardiaceae) Stem Bark Accelerates Bone Fracture Healing in Ovariectomized Wistar Rat"

“…Rich (Anacardiaceae) is distributed in Africa and used to treat various ailments including musculoskeletal disorders and gynaecological complains [17,18], to enhance fertility and facilitate parturition [19]. In vitro, the aqueous and methanolic extracts of stem bark induced uterotonic effects through oxytocin receptors [19]. Chronic, non-resolving inflammation is detrimental to fracture healing [20].…”