Uterine Tissue Metabonomics Combined with 16S rRNA Gene Sequencing To Analyze the Changes of Gut Microbiota in Mice with Endometritis and the Intervention Effect of Tau Interferon

Xue, Guanhong; Zheng, Zhaohui; Liang, Xiaoben; Yan, Zheng; Wu, Haichong

doi:10.1128/spectrum.00409-23

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Studies have shown that the relative abundance of Enterorhabdus in intestinal microbiota is related to glycolipid metabolism ( Huang et al., 2023 ; Lahtinen et al., 2023 ), and Enterorhabdus abundance in PCOS mice is positively correlated with T level and blood glucose ( Li Y. et al., 2023 ). Interferon may affect the expression level of important metabolites in uterine tissue by reducing the abundance of Enterorhabdus in intestinal microbiota, thus alleviating the injury of endometritis in mice ( Xue et al., 2023 ). Lactobacillus has a protective effect on the ovaries, restoring luteinizing hormone, follicle-stimulating hormone, and testosterone levels, upregating short-chain fatty acid levels, and improving intestinal microbiota disorders ( He et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling intestinal microbiota’s dominance in polycystic ovary syndrome pathogenesis over vaginal microbiota

Yu,

Li,

Yang

2024

Front. Cell. Infect. Microbiol.

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BackgroundPolycystic ovary syndrome (PCOS) is a prevalent endocrine disease in women, intricately linked to hormonal imbalances. The microbiota composition plays a pivotal role in influencing hormonal levels within the body. In this study, we utilized a murine model to investigate how intestinal and vaginal microbiota interact with hormones in the development of PCOS.MethodsTwenty female mice were randomly assigned to the normal group (N) and the model group (P), where the latter received daily subcutaneous injections of 0.1 mL DHEA (6 mg/100 g). Throughout the experiment, we evaluated the PCOS mouse model by estrus cycle, serum total testosterone (T), prolactin (PRL) and luteinizing hormone (LH) levels, and ovarian pathological morphology. The microbial composition in both intestinal content and vaginal microbiota were studied by 16S rRNA gene high-throughput sequencing.ResultsCompared with the N group, the P group showed significant increases in body weight, T, and PRL, with significant decrease in LH. Ovaries exhibited polycystic changes, and the estrous cycle was disrupted. The intestinal microbiota result shows that Chao1, ACE, Shannon and Simpson indexes were decreased, Desulfobacterota and Acidobacteriota were increased, and Muribaculaceae, Limosilactobacillus and Lactobacillus were decreased in the P group. T was significantly positively correlated with Enterorhabdus, and LH was significantly positively correlated with Lactobacillus. The analysis of vaginal microbiota revealed no significant changes in Chao1, ACE, Shannon, and Simpson indices. However, there were increased in Firmicutes, Bacteroidota, Actinobacteriota, Streptococcus, and Muribaculaceae. Particularly, Rodentibacter displayed a robust negative correlation with other components of the vaginal microbiota.ConclusionTherefore, the response of the intestinal microbiota to PCOS is more significant than that of the vaginal microbiota. The intestinal microbiota is likely involved in the development of PCOS through its participation in hormonal regulation.

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Section: Discussionmentioning

confidence: 99%

Unraveling intestinal microbiota’s dominance in polycystic ovary syndrome pathogenesis over vaginal microbiota

Yu,

Li,

Yang

2024

Front. Cell. Infect. Microbiol.

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“…To the level of genus, Muribaculum , Akkermansia, and Parasutterella are closely related to anti-inflammation, and a diminished level of Muribaculaceae is a critical contributor to colitis pathogenesis Eubacterium_xylanophilum_group , and Enterorhabdus act a pro-inflammatory role in the inflammation of gut. These results further confirmed that the alleviation of intestinal inflammation and the repair of the barrier by SAL were tightly linked to gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

Salidroside Alleviates Furan-Induced Impaired Gut Barrier and Inflammation via Gut Microbiota–SCFA–TLR4 Signaling

Wang,

Li,

et al. 2024

J. Agric. Food Chem.

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As a food contaminant that can be quickly absorbed through the gastrointestinal system, furan has been shown to disrupt the intestinal flora and barrier. Investigation of the intestinal toxicity mechanism of furan is of great significance to health. We previously identified the regulatory impact of salidroside (SAL) against furan-provoked intestinal damage, and the present work further explored whether the alleviating effect of SAL against furan-caused intestinal injury was based on the intestinal flora; three models, normal, pseudo-germ-free, and fecal microbiota transplantation (FMT), were established, and the changes in intestinal morphology, barrier, and inflammation were observed. Moreover, 16S rDNA sequencing observed the variation of the fecal flora associated with inflammation and short-chain fatty acids (SCFAs). Results obtained from the LC−MS/MS suggested that SAL increased furan-inhibited SCFA levels, activated the mRNA expressions of SCFA receptors (GPR41, GPR43, and GPR109A), and inhibited the furan-activated TLR4/MyD88/NF-κB signaling. Analysis of protein−protein interaction further confirmed the aforementioned effects of SAL, which inhibited furan-induced barrier damage and intestinal inflammation.

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“…It is mainly used to study the molecular relationship between nutrients and biochemical processes, to analyze the effects of food nutrients on metabolism, and to study the cross-genomic metabolite interactions between mammalian hosts and gut microflora as depicted in Fig. 3 [149][150][151]. Metabolites in body fluids are usually influenced by endogenous and exogenous factors, endogenous factors such as gender, age, underlying diseases, etc.…”

Section: Nutrimetabonomicsmentioning

confidence: 99%

Review on analytical technologies and applications in metabolomics

MENG,

LIU,

et al. 2024

BIOCELL

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Over the past decade, the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry, nuclear magnetic resonance, and multivariate statistics. Currently, metabolomics garners widespread application across diverse fields including drug research and development, early disease detection, toxicology, food and nutrition science, biology, prescription, and chinmedomics, among others.Metabolomics serves as an effective characterization technique, offering insights into physiological process alterations in vivo. These changes may result from various exogenous factors like environmental conditions, stress, medications, as well as endogenous elements including genetic and protein-based influences. The potential scientific outcomes gleaned from these insights have catalyzed the formulation of innovative methods, poised to further broaden the scope of this domain. Today, metabolomics has evolved into a valuable and widely accepted instrument in the life sciences. However, comprehensive reviews focusing on the sample preparation and analytical methodologies employed in metabolomics within the life sciences are surprisingly scant. This review aims to fill that gap, providing an overview of current trends and recent advancements in metabolomics. Particular emphasis is placed on sample preparation, sophisticated analytical techniques, and their applications in life science research.

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Uterine Tissue Metabonomics Combined with 16S rRNA Gene Sequencing To Analyze the Changes of Gut Microbiota in Mice with Endometritis and the Intervention Effect of Tau Interferon

Cited by 6 publications

References 37 publications

Unraveling intestinal microbiota’s dominance in polycystic ovary syndrome pathogenesis over vaginal microbiota

Unraveling intestinal microbiota’s dominance in polycystic ovary syndrome pathogenesis over vaginal microbiota

Salidroside Alleviates Furan-Induced Impaired Gut Barrier and Inflammation via Gut Microbiota–SCFA–TLR4 Signaling

Review on analytical technologies and applications in metabolomics

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