Uterine lavage is efficient to recover endometrial cytology sample and does not interfere with fertility rate after artificial insemination in cows

Thomé, Helder Esteves; Arruda, Rubens Paes de; Oliveira, Bruna Marcele Martins de; Filho, Milton Maturana; Oliveira, Guilherme R.; Guimarães, Carina de Fátima; Balieiro, J. C. C.; Azedo, Milton Ricardo; Pogliani, Fábio Celidônio; Celeghini, Eneiva Carla Carvalho

doi:10.1016/j.theriogenology.2016.01.014

“…In a randomised controlled study, Thome et al (2016) evaluated the effect of collecting endometrial cells by uterine lavage in postpartum Nellore cows (50-70 days postpartum). In 35 cows, the uterine lavage was performed 4 h after timed-artificial insemination whilst 93 were not sampled.…”

Section: Resultsmentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

¹

,

Satake

²

,

Lyons

³

et al. 2017

Reprod. Fertil. Dev.

0

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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“…The characteristics of the selected studies are summarised in Table 1.2. Three of these were prospective cohort studies (Kaufmann et al, 2009, Cheong et al, 2012, two studies were randomized controlled studies (RCS) (Etherington et al, 1988, Thome et al, 2016 and one a case-control study (Goshen et al, 2012). In the prospective cohort studies, the experiments compared PR of cytobrush (Kaufmann et al, 2009), uterine flushing (Cheong et al, 2012) or uterine flushing and EB with PR in non-sampled animals.…”

Section: Resultsmentioning

confidence: 99%

“…In the prospective cohort studies, the experiments compared PR of cytobrush (Kaufmann et al, 2009), uterine flushing (Cheong et al, 2012) or uterine flushing and EB with PR in non-sampled animals. In the RCS, one study compared EB (Etherington et al, 1988) and the other compared uterine flushing (Thome et al, 2016) with PR with no uterine sampling. In the case-control study (Goshen et al, 2012), the effect of EB in postpartum cows was compared with non-biopsied cows.…”

Section: Resultsmentioning

confidence: 99%

“…The heterogeneity of the selected studies in terms of type of cattle used (dairy and beef), physiological stage during sampling (early postpartum (Etherington et al, 1988) or late postpartum > 45 days (Goshen et al, 2012) and the interval from sampling to breeding (i.e. 4 h (Kaufmann et al, 2009, Thome et al, 2016 or. 20-40 days (Cheong et al, 2012, Goshen et al, 2012 did not allow comparison between different methods to determine whether one method affects the PR more than any other.…”

Section: Discussionmentioning

confidence: 99%

“…Color Doppler transrectal ultrasonography has been used in cattle to assess uterine blood flow after endometrial injury as an indicator of inflammatory response after artificial insemination or endometrial sampling , Thome et al, 2016, Debertolis et al, 2016). An increase in uterine tissue perfusion was observed within 4 h of the procedure but the uterine hemodynamic returned to baseline by 24 h, indicating that these procedures may induce a short acute inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

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Effect of endometrial biopsy on uterine health in tropically adapted beef cattle

Garzon¹,

Orlando²

1

0

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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Uterine Cytology

Krimer¹,

Miller²

2020

Veterinary Cytology

0

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Uterine lavage is efficient to recover endometrial cytology sample and does not interfere with fertility rate after artificial insemination in cows

Cited by 6 publications

References 20 publications

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Effect of endometrial biopsy on uterine health in tropically adapted beef cattle

Uterine Cytology

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