Uterine contractility is strongly influenced by steroids and glucose metabolism: an<b><i>in vitro</i></b>study on bovine myometrium

Rizzo, Annalisa; Angioni, Stefano; Spedicato, Massimo; Minoia, Giuseppe; Mutinati, Maddalena; Trisolini, Carmelinda; Sciorsci, Raffaele Luigi

doi:10.3109/09513590.2010.507293

Cited by 14 publications

(8 citation statements)

References 31 publications

(35 reference statements)

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“…At all stages of the experiment, the amplitude and frequency of contraction baseline showed a statistically significant difference between the follicular and the luteal phases (Tables 1-6), as reported by previous work [63,64].…”

Section: Resultssupporting

confidence: 85%

Modulatory effect of three antibiotics on uterus bovine contractility in vitro and likely therapeutic approaches in reproduction

et al. 2014

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Section: Resultssupporting

confidence: 85%

Modulatory effect of three antibiotics on uterus bovine contractility in vitro and likely therapeutic approaches in reproduction

et al. 2014

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“…Glucose uptake and metabolism by pre-embryos is essential for blastulation [ 4 , 5 ] and hatching [ 6 ]. Moreover, endometrial decidualization is dependent upon glucose metabolism [ 7 ], and glucose is the preferred energy substrate of the myometrium [ 8 , 9 ].…”

mentioning

confidence: 99%

Uterine glycogen metabolism in mink during estrus, embryonic diapause and pregnancy

Dean

Hunt

McDougall

et al. 2014

Journal of Reproduction and Development

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We have determined uterine glycogen content, metabolizing enzyme expression and activity in the mink, a species that exhibits obligatory embryonic diapause, resulting in delayed implantation. Gross uterine glycogen concentrations were highest in estrus, decreased 50% by diapause and 90% in pregnancy (P ≤ 0.05). Endometrial glycogen deposits, which localized primarily to glandular and luminal epithelia, decreased 99% between estrus and diapause (P ≤ 0.05) and were nearly undetectable in pregnancy. Glycogen synthase and phosphorylase proteins were most abundant in the glandular epithelia. Glycogen phosphorylase activity (total) in uterine homogenates was higher during estrus and diapause, than pregnancy. While glycogen phosphorylase protein was detected during estrus and diapause, glycogen synthase was almost undetectable after estrus, which probably contributed to a higher glycogenolysis / glycogenesis ratio during diapause. Uterine glucose-6-phosphatase 3 gene expression was greater during diapause, when compared to estrus (P ≤ 0.05) and supports the hypothesis that glucose-6-phosphate resulting from phosphorylase activity was dephosphorylated in preparation for export into the uterine lumen. The relatively high amount of hexokinase-1 protein detected in the luminal epithelia during estrus and diapause may have contributed to glucose trapping after endometrial glycogen reserves were depleted. Collectively, our findings suggest to us that endometrial glycogen reserves may be an important source of energy, supporting uterine and conceptus metabolism up to the diapausing blastocyst stage. As a result, the size of uterine glycogen reserves accumulated prior to mating may in part, determine the number of embryos that survive to the blastocyst stage, and ultimately litter size.

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“…Based on our time course results, the chosen at-birth collection time point may have missed the period of perinatal elevation, which may show differences between dystocic and eutocic ewes and epistatic mechanisms that tightly control blood glucose levels may have adjusted this difference already, if it had been there. Glucose appears to be the main energy source for myometrial cells [ 32 , 33 ]. During birth, glucose is mobilised via increased cortisol and adrenaline as a result of pain and stress [ 34 ], ensuring the myometrium has enough energy to fuel the intense activity of birth.…”

Section: Discussionmentioning

confidence: 99%

Using Pen-Side Measurable Blood Parameters to Predict or Identify Dystocic Lambing Events

Redfearn

McNally

Brewer

et al. 2022

Biology

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Dystocia is the greatest contributor to neonatal lamb mortality in Australia and poses significant welfare and economic concerns worldwide. In this study, we set out to investigate whether pen-side analysis technology could be employed to detect blood parameters predictive of dystocic labour events in sheep. In a pilot trial, we collected and analysed blood samples in pen-side assays for glucose, lactate, pH, pCO2, pO2, base excess, HCO3, TCO2, sO2, lactate, sodium, potassium, chloride, calcium, urea nitrogen, creatinine, haematocrit, haemoglobin and anion gap. From the pilot data, we identified creatinine, TCO2, chloride and calcium as potentially useful markers. To develop a time course and to establish variability of the selected blood parameters, a time series of samples was collected from 12 ewes, from mid-gestation to 48 h after birth. For the main trial, blood samples were collected at mid- and late gestation for glucose determination and for the full set of blood parameters at three time points before, at and after birth. Possible predictors of lambing difficulty were chloride, haematocrit and haemoglobin, sampled one week before birth; creatinine, sampled at birth; and blood pH and base excess after birth. In conclusion, we found that pen-side analysis of blood markers showed promise in identifying dystocic lambing events.

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Uterine contractility is strongly influenced by steroids and glucose metabolism: anin vitrostudy on bovine myometrium

Abstract: This study confirms the importance of glucose for uterine contractility and, moreover, it underlines different patterns of contraction with regard to the hormonal status.

Cited by 14 publications

References 31 publications

Modulatory effect of three antibiotics on uterus bovine contractility in vitro and likely therapeutic approaches in reproduction

Modulatory effect of three antibiotics on uterus bovine contractility in vitro and likely therapeutic approaches in reproduction

Uterine glycogen metabolism in mink during estrus, embryonic diapause and pregnancy

Using Pen-Side Measurable Blood Parameters to Predict or Identify Dystocic Lambing Events

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