Uterine clearance and resistance to persistent endometritis in the mare

Troedsson, Mats H.T.

doi:10.1016/s0093-691x(99)00143-0

Cited by 167 publications

(183 citation statements)

References 33 publications

Supporting

Mentioning

136

Contrasting

Unclassified

Order By: Relevance

“…In non-bred heifers, Kastelic et al (1991) scanned the uterus throughout the oestrous cycle and found that the uterine fluid content was about 0.2 ml from Days 10 to Days 16, whereas at Day 18 it increased to up to 3.5 ml. In postpartum cattle and mares that have recently been bred, the presence of fluid in the uterine lumen has been considered a positive sign for the diagnoses of subclinical endometritis (Troedsson, 1999, Barlund et al, 2008. In a recent study, Jaureguiberry et al (2017) found that in 20% of repeat breeder cows (healthy cows with > 3 AI) the presence of fluid (≥2 mm) in uterine lumen, was associated with lower conception rates (odds ratio=0.46).…”

Section: Non-invasive Uterine Assessment Of Endometrial Receptivitymentioning

confidence: 99%

See 1 more Smart Citation

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

show abstract

Section: Non-invasive Uterine Assessment Of Endometrial Receptivitymentioning

confidence: 99%

“…In postpartum cattle and mares that have recently been bred, the presence of fluid in the uterine lumen has been associated with bacterial growth and is considered a positive sign for the diagnoses of subclinical endometritis (Troedsson, 1999, Barlund et al, 2008.…”

Section: Introductionmentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

“…A patologia pode ser classificada em endometrite infecciosa, endometrite persistente induzida pelo coito e endometrite crônica também denominada endometriose (2) . A etiologia e fatores predisponentes da endometrite na égua envolvem defeitos anatômicos na conformação vulvar, histórico de partos distócicos, retenção de placenta, infecções iatrogênicas e falhas nos mecanismos fisiológicos de clearence uterino após a monta natural ou inseminação artificial (3,4) .…”

Section: Introductionunclassified

Sensibilidade Do Laminocultivo Dermatobac® Para O Diagnóstico Da Endometrite Fúngica Em Éguas

et al. 2015

View full text Add to dashboard Cite

ResumoDentre os diferentes fatores etiológicos, a endometrite fúngica representa uma causa frequente de infertilidade na égua. Nesse sentido, dois experimentos foram realizados para avaliar a sensibilidade do Dermatobac®, sistema originalmente desenvolvido para o isolamento de fungos de interesse em Medicina Humana. No Experimento 1, cepas padrão de Candida (n=4) e Aspergyllus (n=1) foram semeadas assepticamente e em duplicata no sistema Dermatobac®, acompanhando-se o crescimento fúngico a cada 24 horas por 7 dias. Para o Experimento 2, foram selecionadas 34 éguas, submetidas à colheita de material uterino para o plaqueamento em Dermatobac® e realização de esfregaços para pesquisa direta de fungos (Controle). No Experimento 1, foi possível a observação macroscópica de todas as cepas de Candida e Aspergyllus a partir de 24 horas do início do cultivo microbiológico. No Experimento 2, foi constatada a presença de leveduras nas lâminas citológicas de cinco animais (14,71%), resultados também confirmados na semeadura em Dermatobac® (sensibilidade de 100%). Foram observados cinco casos falso-positivos caracterizados pelo crescimento microbiológico em sistema Dermatobac® não confirmados através da citologia endometrial, resultados que determinaram a especificidade de 85%. No entanto, todos os casos falso-positivos foram atribuídos a microrganismos contaminantes que apresentaram morfologia diferenciada e tempo de crescimento superior (P<0,05) ao apresentado pelos principais agentes etiológicos das endometrites fúngicas equinas. Conclui-se que o sistema Dermatobac® é eficiente para o diagnóstico macroscópico da endometrite fúngica em equinos, gerando informações visuais a partir de 24 horas de cultivo. Palavras-Chave: Dermatobac®; diagnóstico; éguas; endometrite; fungos. AbstractAmong different etiologic factors, fungal endometritis is a common cause of decreased mare's fertility. Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system

show abstract

“…The introduction of these antigenic agents results in the activation of the complement cascade characterized by an intraluminal influx of fluid containing predominantly polymorphonuclear neutrophils (PMNs). The inflammation, along with coordinated contractions of the myometrium, is the mechanism by which the uterus attempts to rid itself of non-viable spermatozoa and other contaminants introduced during the mating process (Asbury et al 1982, Troedsson 1999, Watson 2000, Portus et al 2005, Troedsson 2006). This process begins within 30 min after breeding and resolves within 24-36 hours.…”

mentioning

confidence: 99%

“…This process begins within 30 min after breeding and resolves within 24-36 hours. The inflammation is considered a pathological process if it is not resolved within 36-48 hours (Troedsson 1999).…”

mentioning

confidence: 99%

The phagocytic function of blood-derived polymorphonuclear neutrophils after administration of dexamethasone for the modulation of post-breeding endometritis in the mare

Öhman¹,

Klein²,

Doty³

et al. 2011

PHK

View full text Add to dashboard Cite

Uterine clearance and resistance to persistent endometritis in the mare

Cited by 167 publications

References 33 publications

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Sensibilidade Do Laminocultivo Dermatobac® Para O Diagnóstico Da Endometrite Fúngica Em Éguas

The phagocytic function of blood-derived polymorphonuclear neutrophils after administration of dexamethasone for the modulation of post-breeding endometritis in the mare

Contact Info

Product

Resources

About