Ustilaginoidin D induces hepatotoxicity and behaviour aberrations in zebrafish larvae

Wang, Bo; Liu, Ling; Li, Yuejiao; Zou, Jiaying; Li, Dayong; Zhao, Dan; Li, Wei; Sun, Wenxian

doi:10.1016/j.tox.2021.152786

Cited by 21 publications

(14 citation statements)

References 53 publications

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“…virens infects stamen filaments and colonizes surrounding rice florets to form dark-green false smut balls covered with powdery chlamydospores at the late infection stage . False smut balls generate a large number of poisonous mycotoxins, including ustiloxins, ustilaginoidins, and sorbicillinoids, which threatens the health of animals and human. ,, Both ustiloxins, a group of cyclopeptide mycotoxins, and ustilaginoidins, the derivatives of bis-naphtho-γ-pyrones, have been shown to be toxic to zebrafish. , Ustilaginoidins exhibit a variety of biological activities, including cytotoxicity to human oral epidermoid carcinoma, inhibitory effects on mitochondrial respiration, certain toxicity and teratogenicity to embryonic limb bud and midbrain cells in mouse, and antibacterial and immunological inhibitory activities. , As a new group of mycotoxins in U. virens, sorbicillinoids have cytotoxic, phytotoxic, and antimicrobial activities .…”

Section: Introductionmentioning

confidence: 99%

“…5,7 Ustilaginoidins exhibit a variety of biological activities, including cytotoxicity to human oral epidermoid carcinoma, inhibitory effects on mitochondrial respiration, certain toxicity and teratogenicity to embryonic limb bud and midbrain cells in mouse, and antibacterial and immunological inhibitory activities. 3,5 As a new group of mycotoxins in U. virens, sorbicillinoids have cytotoxic, phytotoxic, and antimicrobial activities. 8 Therefore, it is important to reveal the regulatory mechanism of the mycotoxin biosynthesis in U. virens.…”

Section: ■ Introductionmentioning

confidence: 99%

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Histone Deacetylase UvHST2 Is a Global Regulator of Secondary Metabolism in Ustilaginoidea virens

Liu,

Wang,

Duan

et al. 2023

J. Agric. Food Chem.

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Ustilaginoidea virens, the causal agent of rice false smut, produces a large amount of mycotoxins, including ustilaginoidins and sorbicillinoids. However, little is known about the regulatory mechanism of mycotoxin biosynthesis inU. virens.Here, we demonstrate that the NAD + -dependent histone deacetylase UvHST2 negatively regulates ustilaginoidin biosynthesis. UvHst2 knockout caused retarded hypha growth and reduced conidiation and pathogenicity inU. virens. Transcriptome analysis revealed that the transcription factor genes, transporter genes, and other tailoring genes in eight biosynthetic gene clusters (BGCs) including ustilaginoidin and sorbicillinoid BGCs were upregulated in ΔUvhst2. Interestingly, the UvHst2 deletion affects alternative splicing. Metabolomics revealed that UvHST2 negatively regulates the biosynthesis of various mycotoxins including ustilaginoidins, sorbicillin, ochratoxin B, zearalenone, and O-M-sterigmatocystin. Combined transcriptome and metabolome analyses uncover that UvHST2 positively regulates pathogenicity but negatively modulates the expression of BGCs involved in secondary metabolism. Collectively, UvHST2 functions as a global regulator of secondary metabolism inU. virens.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Histone Deacetylase UvHST2 Is a Global Regulator of Secondary Metabolism in Ustilaginoidea virens

Liu,

Wang,

Duan

et al. 2023

J. Agric. Food Chem.

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“…The disease not only reduces grain yield but also deteriorates grain quality as a result of the generated mycotoxins (Koyama & Natori, 1988; Koiso et al ., 1994; Nakamura et al ., 1994). Ustilaginoidin D impairs the function of liver, delays yolk sac uptake and alters movement behavior in zebrafish (Wang et al ., 2021a).…”

Section: Introductionmentioning

confidence: 99%

SnRK1A‐mediated phosphorylation of a cytosolic ATPase positively regulates rice innate immunity and is inhibited by Ustilaginoidea virens effector SCRE1

et al. 2022

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Rice false smut caused by Ustilaginoidea virens is becoming one of the most recalcitrant rice diseases worldwide. However, the molecular mechanisms underlying rice immunity against U. virens remain unknown.Using genetic, biochemical and disease resistance assays, we demonstrated that the xb24 knockout lines generated in non-Xa21 rice background exhibit an enhanced susceptibility to the fungal pathogens U. virens and Magnaporthe oryzae. Consistently, flg22-and chitininduced oxidative burst and expression of pathogenesis-related genes in the xb24 knockout lines were greatly attenuated.As a central mediator of energy signaling, SnRK1A interacts with and phosphorylates XB24 at Thr83 residue to promote ATPase activity. SnRK1A is activated by pathogen-associated molecular patterns and positively regulates plant immune responses and disease resistance. Furthermore, the virulence effector SCRE1 in U. virens targets host ATPase XB24. The interaction inhibits ATPase activity of XB24 by blocking ATP binding to XB24. Meanwhile, SCRE1 outcompetes SnRK1A for XB24 binding, and thereby suppresses SnRK1A-mediated phosphorylation and ATPase activity of XB24.Our results indicate that the conserved SnRK1A-XB24 module in multiple crop plants positively contributes to plant immunity and uncover an unidentified molecular strategy to promote infection in U. virens and a novel host target in fungal pathogenesis.

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“…U. virens infects rice flowers and colonizes the inner flowers with mycelia, which are eventually transformed into false smut balls covered with yellow or dark green powdery chlamydospores [ 10 ]. In addition to its negative impacts on rice yield and quality, U. virens can produce poisonous mycotoxins to threaten the health of humans and animals [ 11 , 12 , 13 ] and can also inhibit the radicle and plumule growth of plant seedlings [ 14 , 15 , 16 ]. Up to now, three main types of mycotoxins, including 7 ustiloxins [ 15 , 17 , 18 ], 27 ustilaginoidins [ 14 , 19 , 20 , 21 , 22 ], and 21 sorbicillinoids [ 16 , 23 ] have been identified in U. virens .…”

Section: Introductionmentioning

confidence: 99%

UvSorA and UvSorB Involved in Sorbicillinoid Biosynthesis Contribute to Fungal Development, Stress Response and Phytotoxicity in Ustilaginoidea virens

Zhang

Hou

et al. 2022

IJMS

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Ustilaginoidea virens (teleomorph: Villosiclava virens) is an important fungal pathogen that causes a devastating rice disease. It can produce mycotoxins including sorbicillinoids. The biosynthesis and biological functions of sorbicillinoids have not been reported in U. virens. In this study, we identified a sorbicillinoid biosynthetic gene cluster in which two polyketide synthase genes UvSorA and UvSorB were responsible for sorbicillinoid biosynthesis in U. virens. In ∆UvSorA and ∆UvSorB mutants, the mycelial growth, sporulation and hyphal hydrophobicity were increased dramatically, while the resistances to osmotic pressure, metal cations, and fungicides were reduced. Both phytotoxic activity of rice germinated seeds and cell wall integrity were also reduced. Furthermore, mycelia and cell walls of ∆UvSorA and ∆UvSorB mutants showed alterations of microscopic and submicroscopic structures. In addition, feeding experiment showed that sorbicillinoids could restore mycelial growth, sporulation, and cell wall integrity in ∆UvSorA and ∆UvSorB mutants. The results demonstrated that both UvSorA and UvSorB were responsible for sorbicillinoid biosynthesis in U. virens, and contributed to development (mycelial growth, sporulation, and cell wall integrity), stress responses, and phytotoxicity through sorbicillinoid mediation. It provides an insight into further investigation of biological functions and biosynthesis of sorbicillinoids.

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Ustilaginoidin D induces hepatotoxicity and behaviour aberrations in zebrafish larvae

Cited by 21 publications

References 53 publications

Histone Deacetylase UvHST2 Is a Global Regulator of Secondary Metabolism in Ustilaginoidea virens

Histone Deacetylase UvHST2 Is a Global Regulator of Secondary Metabolism in Ustilaginoidea virens

SnRK1A‐mediated phosphorylation of a cytosolic ATPase positively regulates rice innate immunity and is inhibited by Ustilaginoidea virens effector SCRE1

UvSorA and UvSorB Involved in Sorbicillinoid Biosynthesis Contribute to Fungal Development, Stress Response and Phytotoxicity in Ustilaginoidea virens

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