USPIO-labeled textile materials for non-invasive MR imaging of tissue-engineered vascular grafts

Mertens, Marianne E.; Koch, Sabine; Schuster, P.; Wehner, Jakob; Wu, Zhuojun; Gremse, Felix; Schulz, Volkmar; Rongen, Lisanne; Wolf, Frederic; Frese, J.; Gesché, Valentine; Zandvoort, Marc van; Mela, Petra; Jockenhoevel, Stefan; Kießling, Fabian; Lammers, Twan

doi:10.1016/j.biomaterials.2014.10.076

Cited by 65 publications

(42 citation statements)

References 26 publications

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“…However, this invasive procedure is associated with the potential risks of vascular complications, including dissection, bleeding and pseudoaneurysm, and cannot monitor the longitudinal localization and function of grafts ( 28 ). The applicable non-invasive imaging modality is a superior choice in the tissue engineering experimental stage ( 29 , 30 ). Vascular ultrasound techniques have been validated and are widely used in human subjects ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Accurate and continuous ultrasonography evaluation of small diameter vascular prostheses inï¿½vivo

et al. 2018

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There is a large clinical requirement for novel vascular grafts; however, the development of novel vascular grafts has not been extremely successful to date. The most successful method for the continuous evaluation of vascular grafts in vivo remains unclear. Therefore, an optimal successive, non-invasive imaging modality is necessary for the study of vascular transplantation. In the present study, a common rabbit model of carotid artery defect was utilized. The patency and hemodynamic characteristics of implanted grafts was examined following surgery by color Doppler ultrasound in three modes, including B-mode, color flow map and pulse-Doppler examination. The results revealed that ultrasound had sufficient spatial resolution to generate clear images of the carotid artery of rabbits with or without the implanted grafts. Color Doppler ultrasound may be applied to evaluate and differentiate the patent, stenosis and occlusion of carotid arteries in rabbits with different vascular grafts implanted. Furthermore, color Doppler ultrasound is an optimal imaging modality for continuous evaluation in vivo. It is also possible for some quantitative analyses to be performed, including measuring the diameter of vascular lumens and the flow velocity of the region of interest. The present study suggests vascular ultrasound as the optimum choice for continuous surveillance of vascular prostheses in vivo, which may provide valuable information about the grafts in order to greatly shorten the experimental period.

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Section: Discussionmentioning

confidence: 99%

Accurate and continuous ultrasonography evaluation of small diameter vascular prostheses inï¿½vivo

et al. 2018

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“…(C/I): In a recent study, MRI have been used to facilitate the implantation of TE vascular grafts (TEVG), to longitudinally monitor their localization and function, and to provide noninvasive and quantitative feedback on their remodeling and resorption. Thus, MRI could be useful both during bioreactor cultivation and upon surgical implantation in a noninvasive manner . A recent study demonstrated the effectiveness of quantitative MRI for evaluating the quality of cartilage repair by a 3D scaffold implanted in osteochondral lesions, following allograft chondrocyte implantation over time .…”

Section: Available Imaging Techniquesmentioning

confidence: 99%

Three‐dimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community

Teodori

Crupi

Costa

et al. 2016

Journal of Biophotonics

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Tissue engineering/regenerative medicine (TERM) is an interdisciplinary field that applies the principle of engineering and life sciences to restore/replace damaged tissues/organs with in vitro artificially-created ones. Research on TERM quickly moves forward. Today newest technologies and discoveries, such as 3D-/bio-printing, allow in vitro fabrication of ex-novo made tissues/organs, opening the door to wide and probably never-ending application possibilities, from organ transplant to drug discovery, high content screening and replacement of laboratory animals. Imaging techniques are fundamental tools for the characterization of tissue engineering (TE) products at any stage, from biomaterial/scaffold to construct/organ analysis. Indeed, tissue engineers need versatile imaging methods capable of monitoring not only morphological but also functional and molecular features, allowing three-dimensional (3D) and time-lapse in vivo analysis, in a non-destructive, quantitative, multidimensional analysis of TE constructs, to analyze their pre-implantation quality assessment and their fate after implantation. This review focuses on the newest developments in imaging technologies and applications in the context of requirements of the different steps of the TERM field, describing strengths and weaknesses of the current imaging approaches.

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“…The eventual degradation of biomaterial scaffolds is a key factor to successful cell therapy: would the scaffold persist, it might inhibit the integration of regenerated tissue with the host ( Zhang et al, 2014 ). Recently, approaches have been developed to label scaffolds with SPIO and to monitor their site of implantation, tissue integration and biodegradation ( Mertens et al, 2014 ; Mertens et al, 2015 ). For scaffolds containing gelatin, such as hyaluronic acid hydrogels, a label-free imaging approach has been developed based on its CEST-enhancing properties ( Liang et al, 2015 ) ( Fig.…”

Section: Beyond Cell Tracking: Determining Cellular Function With Mrimentioning

confidence: 99%

Advances in using MRI probes and sensors for in vivo cell tracking as applied to regenerative medicine

Srivastava

Kadayakkara

Bar‐Shir

et al. 2015

Disease Models &Amp; Mechanisms

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The field of molecular and cellular imaging allows molecules and cells to be visualized in vivo non-invasively. It has uses not only as a research tool but in clinical settings as well, for example in monitoring cell-based regenerative therapies, in which cells are transplanted to replace degenerating or damaged tissues, or to restore a physiological function. The success of such cell-based therapies depends on several critical issues, including the route and accuracy of cell transplantation, the fate of cells after transplantation, and the interaction of engrafted cells with the host microenvironment. To assess these issues, it is necessary to monitor transplanted cells non-invasively in real-time. Magnetic resonance imaging (MRI) is a tool uniquely suited to this task, given its ability to image deep inside tissue with high temporal resolution and sensitivity. Extraordinary efforts have recently been made to improve cellular MRI as applied to regenerative medicine, by developing more advanced contrast agents for use as probes and sensors. These advances enable the non-invasive monitoring of cell fate and, more recently, that of the different cellular functions of living cells, such as their enzymatic activity and gene expression, as well as their time point of cell death. We present here a review of recent advancements in the development of these probes and sensors, and of their functioning, applications and limitations.

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USPIO-labeled textile materials for non-invasive MR imaging of tissue-engineered vascular grafts

Cited by 65 publications

References 26 publications

Accurate and continuous ultrasonography evaluation of small diameter vascular prostheses inï¿½vivo

Accurate and continuous ultrasonography evaluation of small diameter vascular prostheses inï¿½vivo

Three‐dimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community

Advances in using MRI probes and sensors for in vivo cell tracking as applied to regenerative medicine

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