Uso da tecnologia de lipossomas no encapsulamento de compostos bioativos - Revisão

Neves, Mateus Tomaz; Santos, Fábio Ribeiro dos; Gonçalves, Daniele Juliana Rodrigues; Fernandes, Janaina Gonçalves; Justino, Heloisa de Fátima Mendes; Júnior, Bruno Ricardo de Castro Leite; Vieira, Érica Nascif Rufino

doi:10.18540/jcecvl7iss4pp13295-01-20e

Cited by 3 publications

(3 citation statements)

References 43 publications

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“…A eficiência de encapsulação (EE) não apresentou diferença significativa, de acordo coma Tabela 1, pode ser interpretada como o percentual de compostos bioativos aprisionado nos lipossomas em comparação com a quantidade inicial desses compostos (Neves et al, 2021).…”

Section: Amostraunclassified

Avaliação do tamanho de particulas lipossomicas em diferentes ensaios

Machado

Souza-Soares

2023

RSD

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Lipossomas compostos por lecitinas de arroz (RL) e soja (SL) foram produzidos por evaporação de fase reversa, utilizando como solvente para solubilização das lecitinas de arroz e soja o Clorofórmio P.A. Com isso o objetivo foi realizar diferentes ensaios para avaliar o tamanho médio e índice de polidispersão de lipossomas com e sem a incorporação do composto fenólico extraído de Spirulina LEB – 18. Os lipossomas puros foram caracterizados em termos de distribuição de tamanho, índice de polidispersidade, e ζ-potencial. Já os Lipossomas em diferentes ensaios: A) As amostras de lipossomas com e sem extratos fenolicos de microalgas foram liofilizadas, em liofilizador (modelo LIOB 003, Biosan), foram submetidas a solubilização em água Ultrapura para a caracterização. B) As amostras de lipossomas com e sem extratos fenolicos de microalgas foram executadas através da centrifugação a 10.000 g por 30 minutos. C) As amostras de lipossomas com e sem extratos fenolicos de microalgas foram submetidas a centrifugação a 10.000 g por 30 min e filtração em filtro de membranas de 0.22 (Filtro Para Seringa 0.22 um Kasvi) e 0.45 µm tamanho do Poro (Membrana Milipore). Logo, estes ensaios foram submetidos a avaliação dos tamanhos e polidispersão. Portanto, foi verificado que o ensaio C obteve-se melhores tamanhos, com os resultados obtidos é possível enfatizar o papel dos lipossomas como importante ferramenta para potencializar o perfil antioxidante de extratos fenólicos de Spirulina sp. LEB-18.

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Section: Amostraunclassified

Avaliação do tamanho de particulas lipossomicas em diferentes ensaios

Machado

Souza-Soares

2023

RSD

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“…Considering the hydrophilic and lipophilic characteristics of the active compounds present in guarana, liposomes were the nanostructures selected in our study as vehicles for the incorporation of guarana powder. This is because the structures allow the incorporation of compounds with different characteristics [20,[61][62][63][64].…”

Section: Stability Study Of Guarana-loaded Liposomesmentioning

confidence: 99%

Potential Use of Guarana-Loaded Liposomes in Topical Applica-Tions: Toxicity Profile Assessment Using Different Skin Cell Lines

Roggia¹,

Gomes²,

Dalcin³

et al. 2023

Preprint

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The objective of this study was to analyze the in vitro stability and toxicity of liposomes containing guarana in skin cell lines. The liposomes were produced by the reverse phase evaporation method containing 1 mg/mL guarana. The stability of the liposomes was evaluated by physical-chemical parameters for up to 90 d using three different storage conditions. The cytotoxicity of guarana (GL), liposomes (B-Lip), and guarana-loaded liposomes (G-Lip) was evaluated on spontaneously immortalized human keratinocyte cell lines (HaCaT), murine swiss albino fibroblasts (3T3), and human fibroblast (1BR.3.G). The evaluation was performed using cellular viability analysis. The techniques used were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red capturing (NRU) and the analyses were conducted after 24, 48, and 72 h exposure of these cells to the different treatments. The G-Lip exhibited physical-chemical stability for 60 d when the samples were stored in a refrigerator. The GL, B-Lip, and G-Lip demonstrated low cytotoxicity in the three cell cultures tested since a low reduction in cell viability was only observed at the highest concentrations. In addition, greater cell damage was observed for B-Lip; however, guarana protects cells from this damage. Thus, G-Lip structures can be considered as promising systems for topical applications.

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“…Considering the hydrophilic and lipophilic characteristics of the active compounds present in guarana, liposomes were the nanostructures selected in our study as vehicles for the incorporation of guarana powder because the structures allow for the incorporation of compounds with different characteristics [20,[64][65][66][67].…”

Section: Stability Study Of Guarana-loaded Liposomesmentioning

confidence: 99%

Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study

et al. 2023

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The objective of this study was to analyze the in vitro stability and toxicity of liposomes containing guarana in skin cell lines. The liposomes were produced by the reverse phase evaporation method containing 1 mg/mL guarana. The stability of the liposomes was evaluated by physical-chemical parameters for up to 90 days using three different storage conditions. The cytotoxicity of guarana (GL), liposomes (B-Lip), and guarana-loaded liposomes (G-Lip) was evaluated on spontaneously immortalized human keratinocyte cell lines (HaCaT), murine Swiss albino fibroblasts (3T3), and human fibroblasts (1BR.3.G). The evaluation was performed using cellular viability analysis. The techniques used were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red capturing (NRU), and the analyses were conducted after 24, 48, and 72 h of exposure of these cells to the different treatments. The G-Lip exhibited physical-chemical stability for 60 days when the samples were stored in a refrigerator. The GL, B-Lip, and G-Lip demonstrated low cytotoxicity in the three different cell cultures tested since a small reduction in cell viability was only observed at the highest concentrations. In addition, greater cell damage was observed for B-Lip; however, guarana protected the cells from this damage. Thus, G-Lip structures can be considered promising systems for topical applications.

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Uso da tecnologia de lipossomas no encapsulamento de compostos bioativos - Revisão

Cited by 3 publications

References 43 publications

Avaliação do tamanho de particulas lipossomicas em diferentes ensaios

Avaliação do tamanho de particulas lipossomicas em diferentes ensaios

Potential Use of Guarana-Loaded Liposomes in Topical Applica-Tions: Toxicity Profile Assessment Using Different Skin Cell Lines

Profiling and Evaluation of the Effect of Guarana-Loaded Liposomes on Different Skin Cell Lines: An In Vitro Study

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