Usnic acid attenuates genomic instability in Chinese hamster ovary (CHO) cells as well as chemical-induced preneoplastic lesions in rat colon

Machado, Nayane Moreira; Ribeiro, Arthur Barcelos; Nicolella, Heloiza Diniz; Ozelin, Saulo Duarte; Silva, Lucas Henrique Domingos da; Guissone, Ana Paula Prado; Rinaldi-Neto, Francisco; Lemos, Igor Lizo Limonti; Furtado, Ricardo Andrade; Cunha, Wilson Roberto; Rezende, Alexandre Azenha Alves de; Spanó, Mário Antônio; Tavares, Denise Crispim

doi:10.1080/15287394.2019.1613274

Cited by 10 publications

(2 citation statements)

References 57 publications

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“…The potential of many natural compounds to modulate the genotoxic effects of xenobiotics has been identified, although they appear to be genotoxic at higher concentrations. [ 38,39 ] Therefore, we evaluated the potential modulating effect of AA on DNA damage induced by different mutagens and the results are shown in Figure 4. The CBPI values obtained for the treated cultures were not significantly different from the control group, revealing the absence of treatment cytotoxicity (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…The potential of many natural compounds to modulate the genotoxic effects of xenobiotics has been identified, although they appear to be genotoxic at higher concentrations. [38,39] Therefore, we evaluated bonds with guanine on the opposing strand. [40] As a topoisomerase II inhibitor, DXR forms a complex between this protein and DNA, inhibiting the processes necessary for DNA synthesis.…”

Section: Aa and Genomic Instability: Protective Effect On Mutagen-imentioning

confidence: 99%

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Influence of Asiatic acid on cell proliferation and DNA damage in vitro and in vivo systems

Ribeiro

Ozelin

Silva

et al. 2021

J Biochem & Molecular Tox

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Asiatic acid (AA) is a triterpene with promising pharmacological activity. In the present study, in vitro and in vivo assays were conducted to understand the effect of AA on cell proliferation and genomic instability. AA was cytotoxic to human tumor cell lines (M059J, HeLa, and MCF‐7), with IC50 values ranging from 13.91 to 111.72 µM. In the case of M059J, AA exhibited selective cytotoxicity after 48 h of treatment (IC50 = 24 µM), decreasing the percentage of cells in the G0/G1 phase, increasing the percentage of cells in the S phase, and inducing apoptosis. A significant increase in chromosomal damage was observed in V79 cell cultures treated with AA (40 µM), revealing genotoxic activity. In contrast, low concentrations (5, 10, and 20 µM) of AA significantly reduced the frequencies of micronuclei induced by the mutagens doxorubicin (DXR), methyl methanesulfonate, and hydrogen peroxide. A reduction of DXR‐induced intracellular free radicals was found in V79 cells treated with AA (10 µM). The antigenotoxic effect of AA (30 mg/kg) was also observed against DXR‐induced chromosomal damage in Swiss mice. Significant reductions in p53 levels were verified in the liver tissue of these animals. Taken together, the data indicate that AA exerted antiproliferative activity in M059J tumor cells, which is probably related to the induction of DNA damage, leading to cell cycle arrest and apoptosis. Additionally, low concentrations of AA exhibited antigenotoxic effects and its antioxidant activity may be responsible, at least in part, for chemoprevention.

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Section: Resultsmentioning

confidence: 99%

“…The potential of many natural compounds to modulate the genotoxic effects of xenobiotics has been identified, although they appear to be genotoxic at higher concentrations. [38,39] Therefore, we evaluated bonds with guanine on the opposing strand. [40] As a topoisomerase II inhibitor, DXR forms a complex between this protein and DNA, inhibiting the processes necessary for DNA synthesis.…”

Section: Aa and Genomic Instability: Protective Effect On Mutagen-imentioning

confidence: 99%

Influence of Asiatic acid on cell proliferation and DNA damage in vitro and in vivo systems

Ribeiro

Ozelin

Silva

et al. 2021

J Biochem & Molecular Tox

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Axenic culture and biosynthesis of secondary compounds in lichen symbiotic fungi, the Parmeliaceae

et al. 2020

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Lichens produce unique secondary metabolites with a rich potential as bioactive compounds. In many cases, the use of these molecules is limited by the low concentration of these compounds in thalli, low growth rate in culture, and changes in chemical patterns between thalli and aposymbiotic culture. In addition, the massive collection of some species of industrial interest can cause damage to lichen diversity and the associated environment. Six lichenized fungi (Arctoparmelia centrifuga, Parmelia saxatilis, Parmelina tiliacea, Platismatia glauca, Xanthoparmelia tinctina, and Usnea ghattensis) with biotechnological interest and belonging to Parmeliaceae have been cultured in order to test culture conditions and obtain enough biomass for further studies. In addition, we analyzed the compounds synthetized in axenic conditions and they were compared with chemosyndromes identified in complete thalli. Arctoparmelia centrifuga, P. saxatilis, P. tiliacea and X. tinctina were successfully cultivated while for P. glauca and U. ghattensis we only obtained sporulation and germination of the spores. The chemical pattern of the compounds secreted into the culture media varied significantly from the chemosyndrome of the whole thallus. Phenolic compounds of pharmacological and industrial interest (usnic acid, aspicilin, α-alectoronic acid, physodic acid, lobaric acid and nordivaricatic acid) and a wide variety of potentially bioactive compounds were obtained during the culture process.

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Review: Usnic acid-induced hepatotoxicity and cell death

Kwong

Wang

2020

Environmental Toxicology and Pharmacology

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Usnic acid attenuates genomic instability in Chinese hamster ovary (CHO) cells as well as chemical-induced preneoplastic lesions in rat colon

Cited by 10 publications

References 57 publications

Influence of Asiatic acid on cell proliferation and DNA damage in vitro and in vivo systems

Influence of Asiatic acid on cell proliferation and DNA damage in vitro and in vivo systems

Axenic culture and biosynthesis of secondary compounds in lichen symbiotic fungi, the Parmeliaceae

Review: Usnic acid-induced hepatotoxicity and cell death

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