Using zebrafish models of leukemia to streamline drug screening and discovery

Deveau, Adam P; Bentley, Victoria L.; Berman, Jason N.

doi:10.1016/j.exphem.2016.09.012

Cited by 29 publications

(18 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zebrafish is an ideal vertebrate model system to conduct bio-reactive compound screens ( Zon and Peterson, 2005 ; Cusick et al, 2012 ; Tamplin et al, 2012 ; Veinotte et al, 2014 ; Rennekamp and Peterson, 2015 ; Deveau et al, 2017 ). The animals are small-sized and lay hundreds of eggs that develop very rapidly, thereby allowing the monitoring of compound activity and biotoxicity in vivo across development.…”

Section: Identification Of Novel Regulatory Mechanisms Of Normal and mentioning

confidence: 99%

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Pater

Trompouki

2018

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Hematopoiesis is an optimal system for studying stem cell maintenance and lineage differentiation under physiological and pathological conditions. In vertebrate organisms, billions of differentiated hematopoietic cells need to be continuously produced to replenish the blood cell pool. Disruptions in this process have immediate consequences for oxygen transport, responses against pathogens, maintenance of hemostasis and vascular integrity. Zebrafish is a widely used and well-established model for studying the hematopoietic system. Several new hematopoietic regulators were identified in genetic and chemical screens using the zebrafish model. Moreover, zebrafish enables in vivo imaging of hematopoietic stem cell generation and differentiation during embryogenesis, and adulthood. Finally, zebrafish has been used to model hematopoietic diseases. Recent technological advances in single-cell transcriptome analysis, epigenetic regulation, proteomics, metabolomics, and processing of large data sets promise to transform the current understanding of normal, abnormal, and malignant hematopoiesis. In this perspective, we discuss how the zebrafish model has proven beneficial for studying physiological and pathological hematopoiesis and how these novel technologies are transforming the field.

show abstract

Section: Identification Of Novel Regulatory Mechanisms Of Normal and mentioning

confidence: 99%

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Pater

Trompouki

2018

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Moreover, in order to provide information about their functional activity, we performed an innovative functional analysis by utilizing zebrafish embryo (Danio rerio), to compare the effect of the drugs on immune system cell activation. Recently, zebrafish has become a very interesting animal model in different fields, including pharmacology and toxicology [21][22][23][24][25][26][27][28]. Aside from small molecule discovery and screening, it is now used for complex biological drugs characterization, such as biosimilars [29,30].…”

Section: Introductionmentioning

confidence: 99%

Parallelism of Chemicostructural Properties between Filgrastim Originator and Three of Its Biosimilar Drugs

Gianoncelli

Bertuzzi

Guarienti

et al. 2019

Journal of Chemistry

View full text Add to dashboard Cite

The approval and granting of marketing authorization for a putative biosimilar is based on strong comparability studies with its biological reference product. This is due to the complexity of the structure and nature of the manufacturing process of biological drugs. Hence, a rigorous analytical workflow for chemical characterization and clinical trials to evaluate the efficacy and safety is required to demonstrate their high similarities to the reference drug. This work is focused on the comparison of the originator of filgrastim with three of its biosimilars by evaluating their structural similarity and biological activity. Qualiquantitative analyses were performed by MALDI-TOF/TOF-MS and RP-HPLC-UV. An innovative functional assay using zebrafish as the animal model was developed to evaluate the biological activities of the drugs. The different analyses performed in this study highlighted the structural similarity of biosimilar drugs and their originator. This result was further confirmed by a similar in vivo biological activity.

show abstract

“…Due to the high level of orthology between the zebrafish and human genome and the many advantages related to laboratory manipulation, zebrafish have become an important research model to understand human disease-related genes (Lieschke and Currie 2007; Phillips and Westerfield 2014; White 2015). In addition, zebrafish have been recognized to be unique vertebrate models amenable to high throughput drug screening and discovery, which cannot readily be done in other vertebrate models and is especially valuable when studying human disease and associated therapeutics (Deveau et al 2016; Liu et al 2016; Williams and Hong 2016). …”

Section: Introductionmentioning

confidence: 99%

Zebrafish Models of Human Disease: Gaining Insight into Human Disease at ZFIN

et al. 2017

View full text Add to dashboard Cite

The Zebrafish Model Organism Database (ZFIN; https://zfin.org) is the central resource for genetic, genomic, and phenotypic data for zebrafish (Danio rerio) research. ZFIN continuously assesses trends in zebrafish research, adding new data types and providing data repositories and tools that members of the research community can use to navigate data. The many research advantages and flexibility of manipulation of zebrafish have made them an increasingly attractive animal to model and study human disease.To facilitate disease-related research, ZFIN developed support to provide human disease information as well as annotation of zebrafish models of human disease. Human disease term pages at ZFIN provide information about disease names, synonyms, and references to other databases as well as a list of publications reporting studies of human diseases in which zebrafish were used. Zebrafish orthologs of human genes that are implicated in human disease etiology are routinely studied to provide an understanding of the molecular basis of disease. Therefore, a list of human genes involved in the disease with their corresponding zebrafish ortholog is displayed on the disease page, with links to additional information regarding the genes and existing mutations. Studying human disease often requires the use of models that recapitulate some or all of the pathologies observed in human diseases. Access to information regarding existing and published models can be critical, because they provide a tractable way to gain insight into the phenotypic outcomes of the disease. ZFIN annotates zebrafish models of human disease and supports retrieval of these published models by listing zebrafish models on the disease term page as well as by providing search interfaces and data download files to access the data. The improvements ZFIN has made to annotate, display, and search data related to human disease, especially zebrafish models for disease and disease-associated gene information, should be helpful to researchers and clinicians considering the use of zebrafish to study human disease.

show abstract

Using zebrafish models of leukemia to streamline drug screening and discovery

Cited by 29 publications

References 70 publications

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis

Parallelism of Chemicostructural Properties between Filgrastim Originator and Three of Its Biosimilar Drugs

Zebrafish Models of Human Disease: Gaining Insight into Human Disease at ZFIN

Contact Info

Product

Resources

About