Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma

Pascoal, Susana; Grissenberger, Sarah; Scheuringer, Eva; Fior, Rita; Ferreira, Miguel Godinho; Distel, Martin

doi:10.1007/978-1-0716-1020-6_19

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…23 Cancer transplantation can be classified as homotransplantation or xenotransplantation, according to whether a tumor tissue block or cell line is transplanted into homogenous or allogeneic animals. 69 , 70 , 71 , 72 , 73 It can also be divided into ectopic and orthotopic transplantation, according to whether the tumor tissue is transplanted to the site corresponding to the primary tumor or other anatomical locations in the recipient animals. 74 , 75 , 76 , 77 …”

Section: Cancer Transplantation Modelmentioning

confidence: 99%

The generation and use of animal models of osteosarcoma in cancer research

Pu¹,

Guo²,

Shi³

et al. 2024

Genes & Diseases

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Section: Cancer Transplantation Modelmentioning

confidence: 99%

The generation and use of animal models of osteosarcoma in cancer research

Pu¹,

Guo²,

Shi³

et al. 2024

Genes & Diseases

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“…E1052110G, Sigma-Aldrich Chemie GmbH, Germany), adjusted to pH 7 with 1 M Tris pH 9.5, in E3). For transplantation anesthetized larvae were aligned on a petri dish lid coated with a solidified 2% agarose solution as described previously [26].…”

Section: Zebrafish Xenotransplantationmentioning

confidence: 99%

High-content drug screening in zebrafish xenografts reveals high efficacy of dual MCL-1/BCL-XL inhibition against Ewing sarcoma

et al. 2023

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“…E1052110G, Sigma-Aldrich Chemie GmbH, Germany), adjusted to pH 7 with 1M Tris pH 9.5, in E3). For transplantation anesthetized larvae were aligned on a petri dish lid coated with a solidified 2% agarose solution as described previously 42 .…”

Section: Preparation Of Cells For Transplantationmentioning

confidence: 99%

Automated compound testing in zebrafish xenografts identifies combined MCL-1 and BCL-X_L inhibition to be effective against Ewing sarcoma

Grissenberger

Sturtzel

Wenninger-Weinzierl

et al. 2021

Preprint

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Ewing sarcoma is a pediatric bone and soft tissue cancer for which new therapies to improve disease outcome and to reduce adverse effects of current standard treatments are urgently needed. To identify new and effective drugs, phenotypic drug screening has proven to be a powerful method and a cancer model ideally suited for this approach is the larval zebrafish xenograft system. Complementing mouse xenografts, zebrafish offer high-througput screening possibilities in an intact complex vertebrate organism. Here, we generated Ewing sarcoma xenografts in zebrafish larvae and established a workflow for automated imaging of xenografts, tumor cell recognition within transplanted zebrafish and quantitative tumor size analysis over consecutive days by high-content imaging. The increased throughput of our in vivo screening setup allowed us to identify combination therapies effective against Ewing sarcoma cells. Especially, combined inhibition of MCL-1 and BCL-XL, two anti-apoptotic proteins, was highly efficient at eradicating tumor cells in our zebrafish xenograft assays with two Ewing sarcoma cell lines and with patient-derived cells. Transcriptional analysis across Ewing sarcoma cell lines and tumors revealed that MCL-1 and BCL2L1, coding for BCL-XL, are the most abundantly expressed anti-apoptotic genes, suggesting that combined MCL-1/BCL-XL inhibition might be a broadly applicable strategy for Ewing sarcoma treatment.

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Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma

Cited by 5 publications

References 11 publications

The generation and use of animal models of osteosarcoma in cancer research

The generation and use of animal models of osteosarcoma in cancer research

High-content drug screening in zebrafish xenografts reveals high efficacy of dual MCL-1/BCL-XL inhibition against Ewing sarcoma

Automated compound testing in zebrafish xenografts identifies combined MCL-1 and BCL-X_L inhibition to be effective against Ewing sarcoma

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Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma

Cited by 5 publications

References 11 publications

The generation and use of animal models of osteosarcoma in cancer research

The generation and use of animal models of osteosarcoma in cancer research

High-content drug screening in zebrafish xenografts reveals high efficacy of dual MCL-1/BCL-XL inhibition against Ewing sarcoma

Automated compound testing in zebrafish xenografts identifies combined MCL-1 and BCL-XL inhibition to be effective against Ewing sarcoma

Contact Info

Product

Resources

About

Automated compound testing in zebrafish xenografts identifies combined MCL-1 and BCL-X_L inhibition to be effective against Ewing sarcoma