Using Trichoderma asperellum to Antagonize Lasiodiplodia theobromae Causing Stem-End Rot Disease on Pomelo (Citrus maxima)

Abstract

Stem-end rot disease has been causing damage to the production of pomelos in Vietnam. The cur-rent study aimed to (i) isolate fungal pathogens causing pomelo stem-end rot disease (PSERD) and (ii) discover Trichoderma spp. that had an antagonistic ability against pathogens under in vitro conditions. Fungi causing PSERD were isolated from pomelo fruits with symptoms of stem-end rot disease and collected from pomelo farms in Ben Tre province, Vietnam. Moreover, 50 fungal strains of Trichoderma spp. also originated from soils of these pomelo farms in Ben Tre province and were dual-tested with the fungal pathogen on the PDA medium. The results demonstrated that 11 pathogenic fungi causing PSERD were isolated from the fruit and showed mycelial growth of roughly 5.33–8.77 cm diameter at 72 h after inoculation. The two fungi that exhibited the fast-est growth, namely, S-P06 and S-P07, were selected. ITS sequencing of the S-P06 and S-P07 fungi resulted in Lasiodiplodia theobromae. All the 50 Trichoderma spp. strains were allowed to antago-nize against the S-P06 and S-P07 strains under in vitro conditions. The greatest antagonistic effi-ciency was found in Trichoderma spp. T-SP19 at 85.4–86.2% and T-SP32 at 84.7–85.4%. The two antagonists were identified as Trichoderma asperellum T-SP19 and T-SP32. The selected strains of Trichoderma asperellum were potent as a biological control for fruit plants.

Pathogenicity of Botryodiplodia theobromae on Sandalwood Stems and Its in Vitro Inhibition by Trichoderma spp.

“…Pemanfaatan agens biokontrol seperti Trichoderma spp. telah dilaporkan sebagai antagonis untuk B. theobromae pada tanaman pala (Susanna et al 2018), makadamia (Li et al 2022), dan pomelo (Khuong et al 2023), tetapi belum pernah diuji pada tanaman cendana. Trichoderma spp.…”

Whole genome sequencing and analysis of the weed pathogen Trichoderma polysporum HZ-31

“…Xylomycetes are soil-borne filamentous fungi that are widely used as a source of biocontrol agents in agriculture 6 . They various have effective antagonistic mechanisms 7 , such as fungal parasitism 8 , antibiotics 9 or competition against plant pathogens 10 and nematodes 11 . Some of the active products of Trichoderma have also been shown to exhibit herbicidal activity in recent years.…”

Whole genome sequencing and analysis of the weed pathogen Trichoderma polysporum HZ-31

“…Xylomycetes are soil-borne lamentous fungi that are widely used as a source of biocontrol agents in agriculture [6] . They have effective antagonistic mechanisms [7] such as fungal parasitism [8] , antibiotics [9] or competition against plant pathogens [10] and nematodes [11] .. Some of the active products of M.…”