Using transgenic modulation of protein synthesis and accumulation to probe protein signaling networks in A<i>rabidopsis thaliana</i>

Warnasooriya, Sankalpi N.; Montgomery, Beronda L.

doi:10.4161/psb.6.9.16437

Cited by 3 publications

(2 citation statements)

References 87 publications

(139 reference statements)

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“…Transgenic modulation of protein synthesis or accumulation has been proposed as a valuable method for investigating protein signaling networks in planta ( Warnasooriya and Montgomery, 2011b ). A transgenic approach targeting degradation of the tetrapyrrole phytochrome chromophore in vivo to regulate accumulation of phytochromes has been developed as a robust tool for investigating phytochrome functions ( Lagarias et al, 1997 ; Montgomery et al, 1999 , 2001 ).…”

Section: Integrating Molecular Approaches Into Understanding Spatiotementioning

confidence: 99%

Spatiotemporal Phytochrome Signaling during Photomorphogenesis: From Physiology to Molecular Mechanisms and Back

Montgomery

2016

Front. Plant Sci.

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Light exposure results in distinct responses in specific seedling tissues during photomorphogenesis. Light promotes growth of cotyledons and leaves, as well as development and elongation of roots, whereas light inhibits elongation of hypocotyls. For distinct plant responses such as shade avoidance, far-red light or shifts in spectral light quality similarly have disparate impacts on distinct plant tissues, resulting in elongation of stems or petioles and a reduction in growth of leaf blades for many species. The physiological bases of such tissue- and organ-specific light responses were initially studied using localized irradiation of specific tissues and organs, or irradiation of dissected plant parts. These historical approaches were used to identify spatial-specific pools of photoreceptors responsible for regulating local, i.e., tissue- or organ-specific, or distal, i.e., interorgan, plant responses. The red/far-red responsive phytochromes have been the most widely studied among photoreceptors in this regard. Whereas, the spatial localization of photoreceptors regulating many tissue- or organ-specific light responses were identified, the underlying signaling networks responsible for mediating the observed responses have not been well defined. Recent approaches used to investigate the molecular bases of spatiotemporal light responses include selective irradiation of plants harboring mutations in specific photoreceptors, tissue-specific expression of photoreceptors, primarily in photoreceptor mutant backgrounds, or tissue-specific biochemical ablation of photoreceptor accumulation. Progressive integration of such approaches for regulating the availability of localized pools of phytochromes with the use of transcriptomic or proteomic analyses for assessing the genes or proteins which these spatially discrete pools of phytochrome regulate is yielding emergent insight into the molecular bases of spatiotemporal phytochrome signaling pathways responsible for regulating spatiotemporal light responses of which we have been aware of at the physiological level for decades. Here, I discuss historical and emerging approaches to elucidating spatiotemporal signaling mediated by phytochromes during photomorphogenesis.

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Section: Integrating Molecular Approaches Into Understanding Spatiotementioning

confidence: 99%

Spatiotemporal Phytochrome Signaling during Photomorphogenesis: From Physiology to Molecular Mechanisms and Back

Montgomery

2016

Front. Plant Sci.

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“…However, 12.2% of the genes in Arabidopsis still lack inserts even the amount of existing insertion mutants have been increased to 385,000 (O'Malley and Ecker 2010); and one of the probable reasons is that T-DNA insertions have a large integration site bias, such as favering intergenic regions (Sessions et al 2002;Alonso et al 2003;Rosso et al 2003). Insertional mutagenesis also has a much lower mutagenic efficiency relative to EMS mutagenesis (Alonso and Ecker 2006;Warnasooriya and Montgomery 2014). The EMS mutagenesis generates multiple types of DNA alterations, although it predominantly generates not only single base-pair substitutions but also small insertions and deletions as demonstrated in a study of more than 1900 EMS-induced mutations in Arabidopsis (Greene et al 2003;Till et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Morphological phenotyping and genetic analyses of a new chemical-mutagenized population of tobacco (Nicotiana tabacum L.)

Wang

Chao

et al. 2017

Planta

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A novel tobacco mutant library was constructed, screened, and characterized as a crucial genetic resource for functional genomics and applied research. A comprehensive mutant library is a fundamental resource for investigating gene functions, especially after the completion of genome sequencing. A new tobacco mutant population induced by ethyl methane sulfonate mutagenesis was developed for functional genomics applications. We isolated 1607 mutant lines and 8610 mutant plants with altered morphological phenotypes from 5513 independent M2 families that consisted of 69,531 M2 plants. The 2196 mutations of abnormal phenotypes in the M2 putative mutants were classified into four groups with 17 major categories and 51 subcategories. More than 60% of the abnormal phenotypes observed fell within the five major categories including plant height, leaf shape, leaf surface, leaf color, and flowering time. The 465 M2 mutants exhibited multiple phenotypes, and 1054 of the 2196 mutations were pleiotropic. Verification of the phenotypes in advanced generations indicated that 70.63% of the M3 lines, 84.87% of the M4 lines, and 95.75% of the M5 lines could transmit original mutant phenotypes of the corresponding M2, M3, and M4 mutant plants. Along with the increased generation of mutants, the ratios of lines inheriting OMPs increased and lines with emerging novel mutant phenotypes decreased. Genetic analyses of 18 stably heritable mutants showed that two mutants were double recessive, five were monogenic recessive, eight presented monogenic dominant inheritance, and three presented semi-dominant inheritance. The pleiotropy pattern, saturability evaluation, research prospects of genome, and phenome of the mutant populations were also discussed. Simultaneously, this novel mutant library provided a fundamental resource for investigating gene functions in tobacco.

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Spatial-specific regulation of root development by phytochromes inArabidopsis thaliana

Warnasooriya

Montgomery

2011

Plant Signaling & Behavior

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Distinct tissues and organs of plants exhibit dissimilar responses to light exposure--cotyledon growth is promoted by light, whereas hypocotyl growth is inhibited by light. Light can have different impacts on root development, including impacting root elongation, morphology, lateral root proliferation and root tropisms. In many cases, light inhibits root elongation. There has been much attention given to whether roots themselves are the sites of photoperception for light that impacts light-dependent growth and development of roots. A number of approaches including photoreceptor localization in planta, localized irradiation and exposure of dissected roots to light have been used to explore the site(s) of light perception for the photoregulation of root development. Such approaches have led to the observation that photoreceptors are localized to roots in many plant species, and that roots are capable of light absorption that can alter morphology and/or gene expression. Our recent results show that localized depletion of phytochrome photoreceptors in Arabidopsis thaliana disrupts root development and root responsiveness to the plant hormone jasmonic acid. Thus, root-localized light perception appears central to organ-specific, photoregulation of growth and development in roots.

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Using transgenic modulation of protein synthesis and accumulation to probe protein signaling networks in Arabidopsis thaliana

Cited by 3 publications

References 87 publications

Spatiotemporal Phytochrome Signaling during Photomorphogenesis: From Physiology to Molecular Mechanisms and Back

Spatiotemporal Phytochrome Signaling during Photomorphogenesis: From Physiology to Molecular Mechanisms and Back

Morphological phenotyping and genetic analyses of a new chemical-mutagenized population of tobacco (Nicotiana tabacum L.)

Spatial-specific regulation of root development by phytochromes inArabidopsis thaliana

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