Using the Serial Ramp Recordings for Rapid Testing of the Generating Ability of Impulse Activity of Isolated Hippocampal Neurons

Va, Yavorsky; Lukyanetz, E. A.

doi:10.15407/fz61.03.019

Cited by 2 publications

(6 citation statements)

References 27 publications

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“…All animal treatment and care procedures were conducted following the National Academy of Sciences of Ukraine and Bogomoletz Institute of Physiology guidelines for the care and use of laboratory animals. The method of obtaining isolated neurons of the rat hippocampus generally corresponded to the method described in our previous works ( Iavors’kyi and Luk”ianets’, 2013 ; Yavorsky and Lukyanetz, 2015 ). Animals (30 rats of 14-day-old) were decapitated after anesthesia with ether; the brain was quickly removed and transferred to a cold (4°C) solution A.…”

Section: Methodsmentioning

confidence: 99%

“…Transmembrane currents and potentials were measured using standard patch-clamp techniques under conditions of perforation of the membrane region under the action of amphotericin-B ( Korn et al, 1991 ; Iavors’kyi and Luk”ianets’, 2013 ; Yavorsky and Lukyanetz, 2015 ). Briefly, patch pipettes were filled with the pipette solution containing 5mg/ml amphotericin-B, previously dissolved in DMSO.…”

Section: Methodsmentioning

confidence: 99%

“…Reproducibility of APs generation was achieved by selecting cells with stable characteristics, unique tuning of stimulation protocols, and constant monitoring of the resting potential in neurons with stabilization of the latter at the same level. Toward the selection of protocols that characterize the regular pulse generation and control the pulsing degradation, we proposed serial testing of cells by rectangular and “ramp”-like stimuli ( Iavors’kyi and Luk”ianets’, 2013 ; Yavorsky and Lukyanetz, 2015 ). Test stimulation with a rectangular waveform consisted of a series of 12 rectangular stimuli at a holding resting potential of membrane −80 mV, with a duration of a single stimulus of 1 s. Neurons responded to stimulation by generating APs, which made it possible to estimate neuronal accommodation properties.…”

Section: Methodsmentioning

confidence: 99%

“…In this case, the washout effect is much smaller, and there is a period in which pulse generation has almost constant characteristics. Thus, an informative method of analyzing the neuron’s impulse activity is to build the APs activation functions of neurons in the protocol with a slow increase in input current (”ramp” protocol) under conditions of quality control and determination of cell generation status ( Yavorsky and Lukyanetz, 2015 ). We determined the peak times of AP and calculated the instantaneous APs frequencies as inverse values of the APs intervals.…”

Section: Methodsmentioning

confidence: 99%

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Influence of amyloid beta on impulse spiking of isolated hippocampal neurons

Yavorsky

Rozumna

Lukyanetz

2023

Front. Cell. Neurosci.

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One of the signs of Alzheimer’s disease (AD) is the formation of β-amyloid plaques, which ultimately lead to the dysfunction of neurons with subsequent neurodegeneration. Although extensive researches have been conducted on the effects of different amyloid conformations such as oligomers and fibrils on neuronal function in isolated cells and circuits, the exact contribution of extracellular beta-amyloid on neurons remains incompletely comprehended. In our experiments, we studied the effect of β-amyloid peptide (Aβ1–42) on the action potential (APs) generation in isolated CA1 hippocampal neurons in perforated patch clamp conditions. Our findings demonstrate that Aβ1–42 affects the generation of APs differently in various hippocampal neurons, albeit with a shared effect of enhancing the firing response of the neurons within a minute of the start of Aβ1–42 application. In the first response type, there was a shift of 20–65% toward smaller values in the firing threshold of action potentials in response to inward current. Conversely, the firing threshold of action potentials was not affected in the second type of response to the application of Aβ1–42. In these neurons, Aβ1-42 caused a moderate increase in the frequency of spiking, up to 15%, with a relatively uniform increase in the frequency of action potentials generation regardless of the level of input current. Obtained data prove the absence of direct short-term negative effect of the Aβ1–42 on APs generation in neurons. Even with increasing the APs generation frequency and lowering the neurons’ activation threshold, neurons were functional. Obtained data can suggest that only the long-acting presence of the Aβ1–42 in the cell environment can cause neuronal dysfunction due to a prolonged increase of APs firing and predisposition to this process.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Influence of amyloid beta on impulse spiking of isolated hippocampal neurons

Yavorsky

Rozumna

Lukyanetz

2023

Front. Cell. Neurosci.

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“…The method of obtaining isolated neurons of the hippocampus of rats in general corresponded to that described in our previous works [15][16][17][18]. Animals (mature rats) decapitated after anesthesia with ether.…”

Section: Isolation Of the Neurons Of The Ca1 Region Of The Hippocampusmentioning

confidence: 99%

Effect of caffeine and coffee diets on calcium signalling in rat hippocampal neurons

Shkryl¹,

Turitska²,

Yavorsky³

et al. 2021

Fiziol Zh

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The effects of long-lasting high concentration coffee and caffeine diets on calcium mobilization in rat hippocampal neurons were studied. Changes in the basal calcium level in the hippocampal neurons of control and experimental rats kept on a coffee or caffeine diet were measured. We also recorded the changes in the Ca2+ transients’ amplitude evoked by membrane depolarization or emptying the Ca2+ depot of the endoplasmic reticulum (ER) induced by caffeine activator of the ryanodine receptors. In rats on a coffee or caffeine diet, the basal Ca2+ level was increased by 7.4% and 11%, respectively, compared to control animals. In these groups, the amplitude of Ca2+ transients increased by 70% and 90%, respectively, of the basal level in response to the membrane depolarization. In the same groups, the amount of Ca2+ released from the ER was increased by two and three times, respectively, compared to the control after activation of ryanodine receptors. We concluded that long-term coffee and caffeine diets in rats cause a significant disruption of the hippocampal neurons’ endoplasmic reticulum function. The diets evoke an increase in Ca2+ concentration in the neurons and an excessive release of Ca2+ in response to excitation. The latter can lead to increased excitability of neurons and their further death from excessive Ca2+ levels.

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Using the Serial Ramp Recordings for Rapid Testing of the Generating Ability of Impulse Activity of Isolated Hippocampal Neurons

Cited by 2 publications

References 27 publications

Influence of amyloid beta on impulse spiking of isolated hippocampal neurons

Influence of amyloid beta on impulse spiking of isolated hippocampal neurons

Effect of caffeine and coffee diets on calcium signalling in rat hippocampal neurons

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