Using the scratch assay to study cell migration in an inquiry-based cell biology lab

Asmus, Stephen E.; Batey, K.; Gale-Butto, Serena N.; Schmitt, Barrie L.

doi:10.1080/00219266.2018.1447004

Cited by 4 publications

(1 citation statement)

References 4 publications

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“…The exercises described here took place during three to four consecutive laboratory periods, which occupied the middle third of 13 total laboratory sessions. In the sessions prior to this module, students worked with Chinese Hamster Ovary (CHO) cells to study topics such as cell migration, 7 and these earlier exercises provided training in the use of cell culture equipment, sterile technique, and microscopy. Prior to each exercise, students were informed of the available reagents, and group members discussed their hypotheses for the cell staining and contractility exercises with the instructor.…”

Section: Methodsmentioning

confidence: 99%

Beating heart cells: Using cultured cardiomyocytes to study cellular structure and contractility in laboratory exercises

Asmus,

Wells,

Montalvo

2023

Biochem Molecular Bio Educ

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Heart muscle cells, or cardiomyocytes, exhibit intrinsic contractility in vitro. We found that commercially‐available mammalian cardiomyocytes serve as an excellent model system for studying the cytoskeleton and cellular contractility, fundamental topics in undergraduate cell and molecular biology courses. Embryonic rat cardiomyocytes were plated on cell culture dishes or glass coverslips and visualized using an inverted phase‐contrast microscope. The cardiomyocytes began contracting within 1–2 days after plating and continued to contract for many weeks, allowing their use in multiple laboratory sessions. Following background reading and instruction, students fixed and triple‐stained the cardiomyocytes to examine the relative distributions of actin filaments and microtubules and the position of nuclei. Analysis and image capture with fluorescence microscopy provided striking examples of highly organized cytoskeletal elements. Students then designed experiments in which cardiomyocyte intrinsic contractility was explored. Changes in contraction rates were examined after treatment with signaling molecules, such as epinephrine. The addition of epinephrine to the culture medium, within a usable concentration window, increased the rate of contraction. These adaptable exercises provide undergraduate cell and molecular biology students with the exciting opportunity to study cardiomyocytes using standard cell culture and microscopy techniques.

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Section: Methodsmentioning

confidence: 99%

Beating heart cells: Using cultured cardiomyocytes to study cellular structure and contractility in laboratory exercises

Asmus,

Wells,

Montalvo

2023

Biochem Molecular Bio Educ

Self Cite

View full text Add to dashboard Cite

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Recent advances and perspectives on natural latex serum and its fractions for biomedical applications

Herculano,

Mussagy,

Guerra

et al. 2024

Biomaterials Advances

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Determination of Total Phenolic and Flavonoid Content in Small Berries

Bellová¹,

Blahútová²,

Culková³

et al. 2021

The American Biology Teacher

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The authors of this article applied the determination of phenolic and flavonoid content in small berries to create a didactic cycle based on student-centered active learning (SCAL) in which students actively participated in the topic while studying and profile-forming as future biology and chemistry teachers. The fundamental part of the proposed didactic cycle is that students solve the following task: Determine the phenolic and flavonoid content in berries (Vaccinium myrtillus L., Vaccinium vitis-idaea L., and Vaccinium corymbosum L.) in your region. The submitted interdisciplinary topic is relevant to everyday life, and it aroused interest and enthusiasm among students. The students had the opportunity to test their theoretical knowledge in practical experimental research, and they attempted to work as researchers.

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Using the scratch assay to study cell migration in an inquiry-based cell biology lab

Cited by 4 publications

References 4 publications

Beating heart cells: Using cultured cardiomyocytes to study cellular structure and contractility in laboratory exercises

Beating heart cells: Using cultured cardiomyocytes to study cellular structure and contractility in laboratory exercises

Recent advances and perspectives on natural latex serum and its fractions for biomedical applications

Determination of Total Phenolic and Flavonoid Content in Small Berries

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