Using spontaneous photon emission to image lipid oxidation patterns in plant tissues

Birtić, Simona; Ksas, Brigitte; Genty, Bernard; Mueller, Martin J.; Triantaphylidès, C.; Havaux, Michel

doi:10.1111/j.1365-313x.2011.04646.x

Cited by 89 publications

(98 citation statements)

References 81 publications

(169 reference statements)

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“…As expected (Birti c et al, 2011), the MGDG solution became luminescent after this oxidation treatment as imaged with a high-sensitivity cooled CCD camera (Fig. 1A).…”

Section: Lipid Protection By Carnosic Acid and Carnosol In Vitrosupporting

confidence: 74%

Carnosic Acid and Carnosol, Two Major Antioxidants of Rosemary, Act through Different Mechanisms

et al. 2017

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Carnosic acid, a phenolic diterpene specific to the Lamiaceae family, is highly abundant in rosemary (Rosmarinus officinalis). Despite numerous industrial and medicinal/pharmaceutical applications of its antioxidative features, this compound in planta and its antioxidant mechanism have received little attention, except a few studies of rosemary plants under natural conditions. In vitro analyses, using high-performance liquid chromatography-ultraviolet and luminescence imaging, revealed that carnosic acid and its major oxidized derivative, carnosol, protect lipids from oxidation. Both compounds preserved linolenic acid and monogalactosyldiacylglycerol from singlet oxygen and from hydroxyl radical. When applied exogenously, they were both able to protect thylakoid membranes prepared from Arabidopsis (Arabidopsis thaliana) leaves against lipid peroxidation. Different levels of carnosic acid and carnosol in two contrasting rosemary varieties correlated with tolerance to lipid peroxidation. Upon reactive oxygen species (ROS) oxidation of lipids, carnosic acid was consumed and oxidized into various derivatives, including into carnosol, while carnosol resisted, suggesting that carnosic acid is a chemical quencher of ROS. The antioxidative function of carnosol relies on another mechanism, occurring directly in the lipid oxidation process. Under oxidative conditions that did not involve ROS generation, carnosol inhibited lipid peroxidation, contrary to carnosic acid. Using spin probes and electron paramagnetic resonance detection, we confirmed that carnosic acid, rather than carnosol, is a ROS quencher. Various oxidized derivatives of carnosic acid were detected in rosemary leaves in low light, indicating chronic oxidation of this compound, and accumulated in plants exposed to stress conditions, in parallel with a loss of carnosic acid, confirming that chemical quenching of ROS by carnosic acid takes place in planta.

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“…As expected (Birti c et al, 2011), the MGDG solution became luminescent after this oxidation treatment as imaged with a high-sensitivity cooled CCD camera (Fig. 1A).…”

Section: Lipid Protection By Carnosic Acid and Carnosol In Vitrosupporting

confidence: 74%

Carnosic Acid and Carnosol, Two Major Antioxidants of Rosemary, Act through Different Mechanisms

et al. 2017

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“…Leaves were dark acclimated for 2 h, and the luminescence emitted from the spontaneous decomposition of lipid peroxides was captured by a highly sensitive liquid N 2 -cooled CCD camera, as previously described (Birtic et al, 2011). The images were treated using Image J software (NIH).…”

Section: Autoluminescence Imagingmentioning

confidence: 99%

The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis

et al. 2017

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Light utilization is finely tuned in photosynthetic organisms to prevent cellular damage. The dissipation of excess absorbed light energy, a process termed nonphotochemical quenching (NPQ), plays an important role in photoprotection. Little is known about the sustained or slowly reversible form(s) of NPQ and whether they are photoprotective, in part due to the lack of mutants. The Arabidopsis thaliana suppressor of quenching1 (soq1) mutant exhibits enhanced sustained NPQ, which we term qH. To identify molecular players involved in qH, we screened for suppressors of soq1 and isolated mutants affecting either chlorophyllide a oxygenase or the chloroplastic lipocalin, now renamed plastid lipocalin (LCNP). Analysis of the mutants confirmed that qH is localized to the peripheral antenna (LHCII) of photosystem II and demonstrated that LCNP is required for qH, either directly (by forming NPQ sites) or indirectly (by modifying the LHCII membrane environment). qH operates under stress conditions such as cold and high light and is photoprotective, as it reduces lipid peroxidation levels. We propose that, under stress conditions, LCNP protects the thylakoid membrane by enabling sustained NPQ in LHCII, thereby preventing singlet oxygen stress.

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“…Imaged autoluminescence signal is attributed to the spontaneous decomposition of lipid peroxides (Havaux et al, 2006;Birtic et al, 2011). Spontaneous photon emission from whole Arabidopsis plants was measured after 2 h dark adaptation with a liquid N 2 cooled chargecoupled device camera, as detailed by Birtic et al (2011).…”

Section: Lipid Peroxidation Analysis and Imagingmentioning

confidence: 99%

Light-Induced Acclimation of the Arabidopsis chlorina1 Mutant to Singlet Oxygen

et al. 2013

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Singlet oxygen ( 1 O 2 ) is a reactive oxygen species that can function as a stress signal in plant leaves leading to programmed cell death. In microalgae, 1 O 2 -induced transcriptomic changes result in acclimation to 1 O 2 . Here, using a chlorophyll b-less Arabidopsis thaliana mutant (chlorina1 [ch1]), we show that this phenomenon can also occur in vascular plants. The ch1 mutant is highly photosensitive due to a selective increase in the release of 1 O 2 by photosystem II. Under photooxidative stress conditions, the gene expression profile of ch1 mutant leaves very much resembled the gene responses to 1 O 2 reported in the Arabidopsis mutant flu. Preexposure of ch1 plants to moderately elevated light intensities eliminated photooxidative damage without suppressing 1 O 2 formation, indicating acclimation to 1 O 2 . Substantial differences in gene expression were observed between acclimation and high-light stress: A number of transcription factors were selectively induced by acclimation, and contrasting effects were observed for the jasmonate pathway. Jasmonate biosynthesis was strongly induced in ch1 mutant plants under high-light stress and was noticeably repressed under acclimation conditions, suggesting the involvement of this hormone in 1 O 2 -induced cell death. This was confirmed by the decreased tolerance to photooxidative damage of jasmonatetreated ch1 plants and by the increased tolerance of the jasmonate-deficient mutant delayed-dehiscence2.

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Using spontaneous photon emission to image lipid oxidation patterns in plant tissues

Cited by 89 publications

References 81 publications

Carnosic Acid and Carnosol, Two Major Antioxidants of Rosemary, Act through Different Mechanisms

Carnosic Acid and Carnosol, Two Major Antioxidants of Rosemary, Act through Different Mechanisms

The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis

Light-Induced Acclimation of the Arabidopsis chlorina1 Mutant to Singlet Oxygen

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