Using small angle scattering (SAS) to structurally characterise peptide and protein self-assembled materials

Guilbaud, J.-B.; Saiani, Alberto

doi:10.1039/c0cs00105h

Cited by 83 publications

(105 citation statements)

References 63 publications

(114 reference statements)

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“…The complex issue of biomacromolecular assembly in solution is also considered. Of particular importance is the review by Guilbaud and Saiani (87) specifically devoted to the peptide-based self-assembled materials and hydrogels. Here, the authors discuss the radius of gyration concept and its connection to bulk material properties, special attention is also paid to the analysis of mass-fractal dimensions and their link to the morphology of assembled materials.…”

Section: Small Angle Scattering In Biomaterials Researchmentioning

confidence: 99%

Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials

Hyland

Taraban

2013

Soft Matter

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The design and engineering of innovative biopolymer-based biomaterials for a variety of biomedical applications should be based on the understanding of the relationship between their nanoscale structure and mechanical properties. Down the road, such understanding could be fundamental to tune the properties of engineered tissues, extracellular matrices for cell delivery and proliferation/differentiation, etc. In this tutorial review, we attempt to show in what way biomaterial structural data can help to understand the bulk material properties. We begin with some background on common types of biopolymers used in biomaterials research, discuss some typical mechanical testing techniques and then review how others in the field of biomaterials have utilized small-angle scattering for material characterization. Detailed examples are then used to show the full range of possible characterization techniques available for biopolymer-based biomaterials. Future developments in the area of material characterization by small-angle scattering will undoubtedly facilitate the use of structural data to control the kinetics of assembly and final properties of prospective biomaterials.

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Section: Small Angle Scattering In Biomaterials Researchmentioning

confidence: 99%

Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials

Hyland

Taraban

2013

Soft Matter

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“…Whereas microscopy techniques such as transmission electron microscopy (TEM) and atomic force microscopy (AFM) are liable to suffer from the presence of sample preparation artefacts, small angle scattering is conducted on the native hydrogel, providing an accurate statistical three dimensional perspective of the actual hydrogel structure. SANS measurements have been extensively used to probe the native structure of peptide-based hydrogels, most often on hydrogel samples which already exhibit a well-defined network151617181920212223242526. Less well studied is small angle scattering on transitions within gelators; however there are examples of SANS being used to examine the transition of a hexapeptide from ribbons to fibers27 and using SANS to look at the evolution of a pyromellitamide organogel over several days28.…”

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confidence: 99%

Controlling self-assembly of diphenylalanine peptides at high pH using heterocyclic capping groups

Martin

Wojciechowski

Robinson

et al. 2017

Sci Rep

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Using small angle neutron scattering (SANS), it is shown that the existence of pre-assembled structures at high pH for a capped diphenylalanine hydrogel is controlled by the selection of N-terminal heterocyclic capping group, namely indole or carbazole. At high pH, changing from a somewhat hydrophilic indole capping group to a more hydrophobic carbazole capping group results in a shift from a high proportion of monomers to self-assembled fibers or wormlike micelles. The presence of these different self-assembled structures at high pH is confirmed through NMR and circular dichroism spectroscopy, scanning probe microscopy and cryogenic transmission electron microscopy.

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“…Small-angle X-ray or neutron scattering are hugely powerful techniques for imaging gels; the imaging can be done in situ. 51 Although access to a beamline at a facility is usually required, the quality of more accessible lab-based X-ray equipment is improving constantly to allow access to good-quality data. The scattering data of course have to be fitted to a model, but the advantages are that these are bulk measurements, representing the sample as a whole.…”

Section: Introductionmentioning

confidence: 99%

Low-Molecular-Weight Gels: The State of the Art

Draper

Adams

2017

Chem

521

469

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Low-molecular-weight gels are currently a hugely important class of materials that are attracting significant interest. These gels are formed when small molecules self-assemble into one-dimensional structures that entangle and cross-link to form a network that is capable of immobilizing the solvent. Here, we critically discuss the current state of the art and highlight two key areas where we believe there is significant untapped potential. The first is the observation that the properties of the gels are highly process dependent, which means that it is possible to access materials with very different properties from a single gelator. Second, using multiple gelators offers the opportunity to prepare materials with a high degree of information content and with a wider range of properties. We aim to spark thought and discussion on these aspects. INTRODUCTIONLow-molecular-weight gels (LMWGs), or supramolecular gels, are a fascinating and useful class of material. The gels arise from the self-assembly of small molecules into long, anisotropic structures, most commonly fibers. [1][2][3][4][5] At a sufficiently high concentration, these fibers entangle or otherwise form cross-links, leading to the network that is able to immobilize the solvent through surface tension and capillary forces. 1,2 These gels differ from permanently covalently cross-linked polymer gels because the cross-linking can be reversed by the input of energy, for example, by heating. 6 LMWGs have been around for many years but are receiving considerable current interest. 4 They are also used in industrial products, 7 although this seems rarely discussed in the academic literature.In addition to the industrial applications, many recent advances and uses are being described. [8][9][10][11][12] The specific self-assembly leading to gel formation can be exploited. For example, the fiber formation is a result of molecular stacking, meaning that the self-assembly leads to aggregates that can be suitable for optoelectronic applications. 13,14 The ready reversibility of gelation can be exploited, for example, to release cells from gels on demand in a manner that does not lead to cell death. 15 Ready gel formation by a simple trigger can also be used to allow easy and efficient gel loading. [16][17][18] Unsurprisingly, therefore, there is significant interest in these materials ( Figure 1). This is a fascinating area and in many ways holds attention because of the difficulties in probing and understanding the gels. The gels arise from assembly across many length scales, and understanding all of these is difficult. At the molecular level, the molecules must interact in a manner that leads to the formation of suitable aggregates that can eventually entangle. Thus, one-dimensional growth must be favored. From this perspective, it is very frustrating that it is often extremely difficult to predict whether a molecule will form a gel or not; indeed, gelation has been described as an empirical science. 4 Structurally similar small molecules can exhibitThe Bigger Pict...

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Using small angle scattering (SAS) to structurally characterise peptide and protein self-assembled materials

Cited by 83 publications

References 63 publications

Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials

Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials

Controlling self-assembly of diphenylalanine peptides at high pH using heterocyclic capping groups

Low-Molecular-Weight Gels: The State of the Art

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