Using silkworms to establish alternative animal models for evaluation of drug-induced tissue injury

Inagaki, Yoshiaki; Matsumoto, Yasuhiko; Sekimizu, Kazuhisa

doi:10.5582/ddt.2016.01023

Cited by 4 publications

(6 citation statements)

References 29 publications

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“…Researchers are using rats and mice effectively to analyse drug‐induced tissue injury, which refers to the damage or harm caused to a particular organ or tissue in the body due to certain drugs or medications (Inagaki et al, 2016). However, they pose problems like cost and ethical issues, which must be addressed.…”

Section: Silkworm B Mori As Antimicrobial Drug Screening and Pathogen...mentioning

confidence: 99%

Silkworm Bombyx mori as a model organism: A review

Ashraf,

Qamar

2023

Physiological Entomology

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Bombyx mori Linnaeus, 1758 (Lepidoptera: Bombycidae), a very important economical insect and backbone of the silk industry, is fully reliant on humans for its life cycle. It has short life span, possessing many genes having high degree of homology with human disease‐causing genes, low breeding and maintenance cost, has less ethical issues associated with it and has also got its genome fully sequenced. Because of these characteristics, it has been recognized as an alternate invertebrate model organism candidate for use in life science research. It has been successfully used as an alternative invertebrate model organism in a variety of scientific domains, including human disease models, environmental monitoring models, epigenetic models and microbial drug screening and discovery models, since the last decade. This newly emerged model has given promising results so far in its short journey and has a tremendous future prospect of establishing itself as a successful model just like the classical invertebrate models, Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae) and Caenorhabditis elegans Maupas, 1900 (Rhabditida: Rhabditidae). The use of the silkworm B. mori as a model organism in areas linked to human health and disease is reviewed here.

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Section: Silkworm B Mori As Antimicrobial Drug Screening and Pathogen...mentioning

confidence: 99%

Silkworm Bombyx mori as a model organism: A review

Ashraf,

Qamar

2023

Physiological Entomology

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“…9) Furthermore, in silkworm experiments, researchers can distinguish between intrahemolymph injection and intramidgut injection. 9,[15][16][17] The former corresponds to intravenous injection in humans, and the latter to oral administration. The injection techniques are important for the evaluation of pharmacokinetics and sample toxicity.…”

Section: Benefits Of Using Insects In Basic Stud-ies For Drug Discoverymentioning

confidence: 99%

“…18,19) Many in vitro and in vivo analyses revealed that the absorption of compounds from the intestinal tract of silkworms is similar to that of mammals. [15][16][17]20) The total clearance, volume of distribution, and half-life values of antimicrobial agents such as chloramphenicol, tetracycline, vancomycin, rifampicin, micafungin, and fluconazole differ by less than 10-fold between silkworms and mammals. 21) Toxicity to animals is quantitatively indicated by the LD 50 value, the dose of a compound required to kill half of an experimental group.…”

Section: Pharmacokinetics and Toxicity Of Com-pounds In Insectsmentioning

confidence: 99%

“…Many infectious disease models using insects such as nematodes, fruit flies, greater wax moths, and silkworms have been established. 9,13,14,17,[25][26][27][28][29][30][31][32][33][34][35][36] These infection models are based on the death of insects with the administration of pathogenic microorganisms. Large-scale in vivo screening has identified virulence genes of pathogens and defense-related genes of hosts.…”

Section: Human Disease Models Using Insectsmentioning

confidence: 99%

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Facilitating Drug Discovery in Human Disease Models Using Insects

Matsumoto

2020

Biological & Pharmaceutical Bulletin

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Drugs are developed through basic studies and clinical trials. In basic studies, researchers seek drug candidates using in vitro evaluation systems and subsequently examine their effectiveness in animal experiments as in vivo evaluations. Drug candidates identified in basic studies are tested to determine whether they are effective against human diseases in clinical trials. However, most drug candidates identified in in vitro evaluation systems do not show therapeutic effects in animal experiments due to pharmacokinetics and toxicity problems in the in vivo evaluations. This review outlines drug discovery using insect disease models that allow us to perform in vivo screening. Since insects have various advantages as experimental animals such as low cost for rearing and few ethical concerns, researchers can perform large-scale in vivo screening to find drug candidates. Silkworms are insects frequently used for studies of drug efficacy, pharmacokinetics, and toxicity. Based on silkworm research, I describe the benefits of using insect disease models for drug discovery. The use of insect disease models for in vivo screening is expected to facilitate drug discovery.

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“…The silkworm is an important economic insect and has been used as a model organism in various research areas, including human medicine, antimicrobial agent screening, environmental safety monitoring, and antitumor studies (Inagaki, Matsumoto, & Sekimizu, ; Nwibo, Hamamoto, Matsumoto, Kaito, & Sekimizu, ). The silkworm is sensitive to environmental pollution, especially to chemical pollutants such as pesticides and heavy metals (Hamamoto, Tonoike, Narushima, Horie, & Sekimizu, ).…”

Section: Introductionmentioning

confidence: 99%

Aluminum toxicity related to SOD and expression of presenilin and CREB in Bombyx mori

Liu

Qian

Chao

et al. 2018

Arch Insect Biochem Physiol

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Aluminum (Al) is an important environmental metal factor that can be potentially associated with pathological changes leading to neurotoxicity. The silkworm, Bombyx mori, is an important economic insect and has also been used as a model organism in various research areas. However, the toxicity of Al on silkworm physiology has not been reported. Here, we comprehensively investigate the toxic effects of Al on the silkworm, focusing on its effects on viability and development, superoxide dismutase (SOD) activity, and the expression of presenilin and cAMP response element-binding protein (CREB) in BmE cells and silkworm larvae. BmE cell viability decreased after treatment with aluminum chloride (AlCl ) in both dose- and time-dependent manners. When AlCl solution was injected into newly hatched fifth instar larvae, both larval weight gain and survival rate were significantly decreased in a manner correlating with AlCl dose and developmental stage. Furthermore, when BmE cells and silkworm larvae were exposed to AlCl , SOD activity decreased significantly relative to the control group, whereas presenilin expression increased more than twofold. Additionally, CREB and phosphorylated CREB (p-CREB) expression in the heads of fifth instar larvae decreased by 28.0% and 50.0%, respectively. These results indicate that Al inhibits the growth and development of silkworms in vitro and in vivo, altering SOD activity and the expressions of presenilin, CREB, and p-CREB. Our data suggest that B. mori can serve as a model animal for studying Al-induced neurotoxicity or neurodegeneration.

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Using silkworms to establish alternative animal models for evaluation of drug-induced tissue injury

Cited by 4 publications

References 29 publications

Silkworm Bombyx mori as a model organism: A review

Silkworm Bombyx mori as a model organism: A review

Facilitating Drug Discovery in Human Disease Models Using Insects

Aluminum toxicity related to SOD and expression of presenilin and CREB in Bombyx mori

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