Using silkworms as a laboratory animal to evaluate medicines and foods

Sekimizu, Kazuhisa; Hamamoto, Hiroshi

doi:10.5582/ddt.2016.01018

Cited by 5 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We selected B. mori as the infection model, since it is a safe and easy-to-handle insect, with reduced maintenance costs, and does not require special devices for bacterial infection and drug administration, thanks to the big size of the larvae [12]. Antibiotics can be administered to the larvae by several routes and in accurate dosage, and the following isolation of organs and hemolymph does not require particular equipment [13]. Moreover, the occurrence of standard larval instars, the synchronous development of the silkworms during the life cycle, and the reduced inter individual variability improve the reproducibility of the experiments.…”

Section: Introductionmentioning

confidence: 99%

A Silkworm Infection Model for In Vivo Study of Glycopeptide Antibiotics

et al. 2020

View full text Add to dashboard Cite

Glycopeptide antibiotics (GPAs) are drugs of last resort for treating infections by Gram-positive bacteria. They inhibit bacterial cell wall assembly by binding to the d-Ala-d-Ala terminus of peptidoglycan precursors, leading to cell lysis. Vancomycin and teicoplanin are first generation GPAs, while dalbavancin is one of the few, recently approved, second generation GPAs. In this paper, we developed an in vivo insect model to compare, for the first time, the efficacy of these three GPAs in curing Staphylococcus aureus infection. Differently from previous reports, Bombyx mori larvae were reared at 37 °C, and the course of infection was monitored, following not only larval survival, but also bacterial load in the insect body, hemocyte activity, phenoloxidase activity, and antimicrobial peptide expression. We demonstrated that the injection of S. aureus into the hemolymph of B. mori larvae led to a marked reduction of their survival rate within 24–48 h. GPAs were not toxic to the larvae and cured S. aureus infection. Dalbavancin was more effective than first generation GPAs. Due to its great advantages (i.e., easy and safe handling, low rearing costs, low antibiotic amount needed for the tests, no restrictions imposed by ethical and regulatory issues), this silkworm infection model could be introduced in preclinical phases—prior to the use of mice—accelerating the discovery/development rate of novel GPAs.

show abstract

Section: Introductionmentioning

confidence: 99%

A Silkworm Infection Model for In Vivo Study of Glycopeptide Antibiotics

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The mechanisms of innate immunity are highly conserved from invertebrates to vertebrates, which makes the silkworm suitable for the evaluation of the innate immune system. We previously reported the use a basic silkworm-based research model for drug discovery and the investigation of new immunostimulators (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the innate immune-stimulating activity of amazake using a silkworm muscle contraction assay

Maruki‐Uchida

Sai

Sekimizu

2017

DD&T

Self Cite

View full text Add to dashboard Cite

We evaluated the innate immune-stimulating activity of amazake using a silkworm muscle contraction assay. Sake cake, a raw material used to make amazake, had high innate immunity-stimulating activity, whereas rice malt, another raw material used to make amazake, did not, even after fermentation. These results suggest that the silkworm muscle contraction assay is a useful tool to screen foods with high innate immune-stimulating activity and that amazake made from sake cake has immunomodulatory potential.

show abstract

Research on the Influencing Factors of Peristalsis Amplitude Based on an in Vitro Bionic Rat Stomach Model

Liang,

Zhao,

et al. 2024

J Bionic Eng

View full text Add to dashboard Cite

Using silkworms as a laboratory animal to evaluate medicines and foods

Cited by 5 publications

References 11 publications

A Silkworm Infection Model for In Vivo Study of Glycopeptide Antibiotics

A Silkworm Infection Model for In Vivo Study of Glycopeptide Antibiotics

Evaluation of the innate immune-stimulating activity of amazake using a silkworm muscle contraction assay

Research on the Influencing Factors of Peristalsis Amplitude Based on an in Vitro Bionic Rat Stomach Model

Contact Info

Product

Resources

About