Using short-read 16S rRNA sequencing of multiple variable regions to generate high-quality results to a species level.
Amy S Graham,
Fadheela Patel,
Francesca Little
et al.
Abstract:Introduction Short-read amplicon sequencing studies have typically focused on 1-2 variable regions of the 16S rRNA gene. Species-level resolution is limited in these studies, as each variable region enables the characterisation of a different subsection of the microbiome. Although long-read sequencing techniques take advantage of all 9 variable regions by sequencing the entire 16S rRNA gene, they are substantially more expensive. This work assessed the feasibility of accurate species-level resolution and repro… Show more
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