Using selected uniform cells in round shape with a micropipette to measure cell adhesion strength on silk fibroin-based materials

Gao, Zhen; Wang, Song; Zhu, Hesun; Su, Caoning; Xu, Guoliang; Lian, Xiaojie

doi:10.1016/j.msec.2007.11.003

Cited by 22 publications

(21 citation statements)

References 32 publications

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“…Micropipette aspiration technique is able to measure various mechanical properties of cells, such as the membrane stiffness of chondrocytes and endothelial cells [ 108 ], the cortical tension of neutrophils [ 108 ], and the adhesion strength of human umbilical vein endothelial cells on different substrates [ 109 ]. Gao et al [ 109 ] used cells in the phase they called the “adhered cells in round shape” phase to eliminate the influences of floating cells, weakly adhered cells, and spread cells and obtained high reproducibility and sensitivity of the measurement on different substrates. They reported that the sensitivity and accuracy of their technique could reach 8 × 10 − 12 N [ 109 ].…”

Section: Types Of Adhesion Studiesmentioning

confidence: 99%

A Review of Cell Adhesion Studies for Biomedical and Biological Applications

Khalili

Ahmad

2015

IJMS

722

545

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Cell adhesion is essential in cell communication and regulation, and is of fundamental importance in the development and maintenance of tissues. The mechanical interactions between a cell and its extracellular matrix (ECM) can influence and control cell behavior and function. The essential function of cell adhesion has created tremendous interests in developing methods for measuring and studying cell adhesion properties. The study of cell adhesion could be categorized into cell adhesion attachment and detachment events. The study of cell adhesion has been widely explored via both events for many important purposes in cellular biology, biomedical, and engineering fields. Cell adhesion attachment and detachment events could be further grouped into the cell population and single cell approach. Various techniques to measure cell adhesion have been applied to many fields of study in order to gain understanding of cell signaling pathways, biomaterial studies for implantable sensors, artificial bone and tooth replacement, the development of tissue-on-a-chip and organ-on-a-chip in tissue engineering, the effects of biochemical treatments and environmental stimuli to the cell adhesion, the potential of drug treatments, cancer metastasis study, and the determination of the adhesion properties of normal and cancerous cells. This review discussed the overview of the available methods to study cell adhesion through attachment and detachment events.

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Section: Types Of Adhesion Studiesmentioning

confidence: 99%

A Review of Cell Adhesion Studies for Biomedical and Biological Applications

Khalili

Ahmad

2015

IJMS

722

545

View full text Add to dashboard Cite

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“…In this case, detachment forces are calculated from the pressure required to suck up a cell into the capillary. According to this technique, the adhesion forces are of the order of 1-10 nN [15,16].…”

Section: Cell Adhesion To the Substratementioning

confidence: 99%

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Ventre

Causa

Netti

2012

J. R. Soc. Interface.

158

153

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The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional biological tissues, advanced cell culture systems, single-cell diagnosis as well as for cell sorting and differentiation. It is well established that crosstalk at the cell–material interface occurs and this has a profound influence on cell behaviour. However, the complete deciphering of the cell–material communication code is still far away. A variety of material surface properties have been reported to affect the strength and the nature of the cell–material interactions, including biological cues, topography and mechanical properties. Novel experimental evidence bears out the hypothesis that these three different signals participate in the same material–cytoskeleton crosstalk pathway via adhesion plaque formation dynamics. In this review, we present the relevant findings on material-induced cell response along with the description of cell behaviour when exposed to arrays of signals—biochemical, topographical and mechanical. Finally, with the aid of literature data, we attempt to draw unifying elements of the material–cytoskeleton–cell fate chain.

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“…The links of the micropipette system and the measuring process had been described previously [21]. Briefly, after ECV-304 cells were incubated on SF and SF/FC films with a density of 5 6 10 5 cells/mL at 37uC for 2.0-2.5 h, the old medium was discarded and fresh medium was added into the dish for micropipette aspirating experiment.…”

Section: Evaluation Of Cell Attachment and Proliferationmentioning

confidence: 99%

Improvements of anticoagulant activities of silk fibroin films with fucoidan

Gao

Wang

Zhu

et al. 2008

Front. Mater. Sci. China

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Fucoidan (FC), an effective anticoagulant constituent extracted from brown algae, was introduced into silk fibroin (SF) for improving its blood compatibility. The SF and SF/FC blend films were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and dynamic contact angle determinator (CA). The in vitro anticoagulant activities of the films were evaluated by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) measurements. The endothelial cell attachment and proliferation viability on the film were assessed by micropipette aspiration technique and MTT assay, respectively. The testing results indicated that the introduction of FC increased the roughness, hydrophilicity and sulfate component of the film surface without impeding the formation of b-sheet conformation in SF. More important, FC brought excellent anticoagulant activity and better endothelial cell affinity to SF. The SF/FC blend film was hopeful to be used as blood-contacting biomaterials.

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Using selected uniform cells in round shape with a micropipette to measure cell adhesion strength on silk fibroin-based materials

Cited by 22 publications

References 32 publications

A Review of Cell Adhesion Studies for Biomedical and Biological Applications

A Review of Cell Adhesion Studies for Biomedical and Biological Applications

Determinants of cell–material crosstalk at the interface: towards engineering of cell instructive materials

Improvements of anticoagulant activities of silk fibroin films with fucoidan

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