Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique to Analyze Genetic Variation in Staphylococcus Aureus Isolated from Different Sources in Babylon Province Hospitals

Banoon, Shaima Rabeea; Kadhim, Zahraa Kamil; Aziz, Zahid S.; Jameel, Zahraa Isam; EWadh, Ruqaya M.J.

doi:10.5958/0976-5506.2019.02624.x

Cited by 2 publications

(2 citation statements)

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“…Staphylococci are Gram-positive cocci, facultative and aerobic bacteria that can be isolated from animals and various environmental sources such as soil, sand, dust, air, natural water, and clothing (Götz et al 2006;Banoon et al 2019;Al-Muhanna et al 2021). Some Staphylococci species are opportunistic, causing various infections such as inflammatory bowel, cystitis, bacteremia, and vaginitis (Muzny et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Bacterial vaginosis pattern and antibiotic susceptibility testing in female patients using high vaginal swabs

Al-Kraety

Al-Muhanna²,

BANOON³

et al. 2022

Biodiversitas

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Abstract. Al-Kraety IAA, Al-Muhanna SG, Banoon SR, Ghasemian A. 2022. Bacterial vaginosis pattern and antibiotic susceptibility testing in female patients using high vaginal swabs. Biodiversitas 23: 2838-2844. Bacterial species found in the vaginal environment encompass a wide variety of species. A common cause of vaginal discharge in women is bacterial vaginosis BV (BV). Various Gram-positive and Gram-negative rod shaped bacteria, including E. coli, Klebsiella spp., Enterococcus spp., Enterobacter spp., Raoultella ornithinolytica, and Staphylococcus spp. contribute significantly to bacterial vaginosis. In this study, vaginal swabs (VS) were obtained from 50 individuals with symptoms of vaginal discharge. The swabs were inoculated on blood, Mannitol, and MacConkey agar culture media. Biochemical tests were performed after an overnight incubation period to determine growth and colonial morphology. In addition to VITEK® 2 compact system and PCR technique by using a 16s RNA gene where all bacteria isolates were positive for this gene. Antibiotic sensitivity was investigated through compact VITEK® 2 and sensitivity cards (AST-P580), (AST-N222), and (AST-GN76). The bacterial isolates including 20 (43.4%) of E. coli, 8 (17.3%) of Klebsiella spp. and 8 (17.3%) of Staphylococcus spp. were investigated in present study. Additionally, 4 (8.6%) isolates of Enterobacter spp., 3 (6.5%) of E. faecalis, and 3 (6.5%) of R. ornithinolytica. The E. coli, Staphylococcus spp., Enterobacter spp., E. faecalis and R. ornithinolytica isolates were found resistant to several antibiotics and considered multi-resistance (MDR).

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Section: Introductionmentioning

confidence: 99%

Bacterial vaginosis pattern and antibiotic susceptibility testing in female patients using high vaginal swabs

Al-Kraety

Al-Muhanna²,

BANOON³

et al. 2022

Biodiversitas

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“…Actinomycetes are renowned for being a rich source of bioactive chemical compounds with great potential for medical and pharmaceutical applications. Although the isolation of terrestrial actinomycetes has been started extensively in the early 1950s, researchers from all around the word are continuing of isolating and discovering of new and rare actinomycetes (Qin et al, 2009;Kruasuwan et al, 2017;Singh et al, 2018;Benhadj et al, 2019;Banoon et al, 2019) for novel pharmaceutical compounds and for combating human pathogens.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Two Rare Actinomycetes Isolated from Mosul, Iraq

Salih

2020

IJOBE

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Rare actinomycetes from diverse habitats are continued to be isolated and screened for their novel bioactive compounds. The present study aims to molecular, morphological and physiological characterisation of two rare actinomycetes isolated from an Iraqi soil. Based on the 16S rRNA gene sequencing, the two isolates were categorized into two different rare genera Actinoplanes and Amycolatopsis that were designated as Actinoplanes sp. MOSUL and Amycolatopsis sp. MOSUL respectively. Phylogenetic trees analyses revealed that Act. sp. MOSUL was closely related strain to Act. xinjiangensis (jgi.1107663; identity 96.75%) and Act. lobatus (AB037006; identity 96.76%), and Amy. sp. MOSUL was most related to Amy. bullii (HQ65173099; identity 99.71%) and Amy. tolypomycina (FNSO01000004; identity 99.26%). The two rare isolates had different morphological properties when grown on International Streptomyces Project (ISP) media, and different physiological and biochemical patterns when grown on Minimal Medium (MM) agar alone or supplemented with different carbon or chemical sources. The ribosomal 16S gene sequences of both Act. sp. MOSUL and Amy. sp. MOSUL strains were submitted to the National Center for Biotechnology Information (NCBI) and deposited in the GenBank database under the accession numbers MN095717 and MN095769 respectively.

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Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique to Analyze Genetic Variation in Staphylococcus Aureus Isolated from Different Sources in Babylon Province Hospitals

Cited by 2 publications

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Bacterial vaginosis pattern and antibiotic susceptibility testing in female patients using high vaginal swabs

Bacterial vaginosis pattern and antibiotic susceptibility testing in female patients using high vaginal swabs

Molecular Identification of Two Rare Actinomycetes Isolated from Mosul, Iraq

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