“…The difference spectra in Fig. 1 showed that both IDA and SCD presented lower intensity for the peaks at 1227, 1375, and 1406 cm −1 , which can be assigned to oxyhemoglobin [33,45,46]; the IDA presented lower intensity for the peaks at 753, 1567, 1622, and 1644 cm −1 , assigned to the hemoglobin molecule [46][47][48][49][50] and higher intensity for the peak at 1131 cm −1 , assigned to the pyrrolic ring of the porphyrin; the SCD presented lower intensity for the peak at 1004 cm −1 , assigned to aromatic ring of amino acids (phenylalanine) [51], and higher intensity for the peaks at 1215, 1358, 1526, 1547, and 1622 cm −1 , assigned to deoxyhemoglobin [45,46,50]. All of these labeled peaks showed statistically significant differences when comparing the three groups (HbA, IDA, and SCD) (one-way ANOVA test, p < 0.05).…”