Using prodigiosin against some gram-positive and gram-negative bacteria and Trypanosoma cruzi

Herráez, Rocío; Mur, Anna; Merlos, Alexandra; Viñas, Miguel; Vinuesa, Teresa

doi:10.1590/1678-9199-jvatitd-2019-0001

Cited by 15 publications

(12 citation statements)

References 27 publications

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“…Arivizhivendhan et al reported the inhibitory activity of S. marcescens produced against P. aeruginosa [ 93 ]. Similar results of antibacterial activities by S. marcescens 2170 PG have also been reported by Herráez et al [ 94 ]. PG produced by S. marcescens UFPEDA 398 showed inhibitory activity against 20 strains of oxacillin-resistant S. aureus .…”

Section: Bioactivity and Application Of Pgsupporting

confidence: 89%

“…The antagonist effect of S. marcescens 2170 produced PG has been tested against parasite Trypanosoma cruzi cells. The results showed that the concentration of PG required to suppress T. cruzi growth was significantly lower than that required for benznidazole (0.25 mg/L and 4.9 mg/L, respectively) [ 94 ]. Isaka et al reported three compounds (metacycloprodigiosin, bafilomycin A 1 , and spectinabilin) extracted from Streptomyces spectabilis BCC 4785 possessed potent antimalarial activity against P. falciparum K1.…”

Section: Bioactivity and Application Of Pgmentioning

confidence: 99%

“…Consequently, the uses of bacterial pigments have expanded much more than synthetic pigments [ 80 ]. Prodiginines (PGs), a family of red microbial pigments, have received much attention because of their considerable bioactivities [ 59 , 60 , 61 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 …”

Section: Introductionmentioning

confidence: 99%

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Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

et al. 2020

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The technology of microbial conversion provides a potential way to exploit compounds of biotechnological potential. The red pigment prodigiosin (PG) and other PG-like pigments from bacteria, majorly from Serratia marcescens, have been reported as bioactive secondary metabolites that can be used in the broad fields of agriculture, fine chemicals, and pharmacy. Increasing PG productivity by investigating the culture conditions especially the inexpensive carbon and nitrogen (C/N) sources has become an important factor for large-scale production. Investigations into the bioactivities and applications of PG and its related compounds have also been given increased attention. To save production cost, chitin and protein-containing fishery byproducts have recently been investigated as the sole C/N source for the production of PG and chitinolytic/proteolytic enzymes. This strategy provides an environmentally-friendly selection using inexpensive C/N sources to produce a high yield of PG together with chitinolytic and proteolytic enzymes by S. marcescens. The review article will provide effective references for production, bioactivity, and application of S. marcescens PG in various fields such as biocontrol agents and potential pharmaceutical drugs.

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Section: Bioactivity and Application Of Pgsupporting

confidence: 89%

Section: Bioactivity and Application Of Pgmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

et al. 2020

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“…Our group recently studied prodigiosin of bacterial origin, reporting its remarkable activity against the epimastigotes of T. cruzi (CL strain, clone B5), with very low IC 50 values (up to 0.8 µM), compared to benznidazole (18 µM). Additionally, we explored the effects of this pigment on the parasite cell surface by atomic force microscopy, which revealed relevant morphological alterations in the size, shape, and roughness [ 26 ]. Based on these encouraging preliminary results, we decided to continue exploring the trypanocidal effect of prodigiosin and related molecules against the intracellular forms of T. cruzi and investigate the targets presumably involved.…”

Section: Introductionmentioning

confidence: 99%

Tambjamines and Prodiginines: Biocidal Activity against Trypanosoma cruzi

et al. 2021

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The aim of this work was to explore new therapeutic options against Chagas disease by the in vitro analysis of the biocidal activities of several tambjamine and prodiginine derivatives, against the Trypanosoma cruzi CLB strain (DTU TcVI). The compounds were initially screened against epimastigotes. The five more active compounds were assayed in intracellular forms. The tambjamine MM3 and both synthetic and natural prodigiosins displayed the highest trypanocidal profiles, with IC50 values of 4.52, 0.46, and 0.54 µM for epimastigotes and 1.9, 0.57, and 0.1 µM for trypomastigotes/amastigotes, respectively. Moreover, the combination treatment of these molecules with benznidazole showed no synergism. Finally, oxygen consumption inhibition determinations performed using high-resolution respirometry, revealed a potent effect of prodigiosin on parasite respiration (73% of inhibition at ½ IC50), suggesting that its mode of action involves the mitochondria. Moreover, its promising selectivity index (50) pointed out an interesting trypanocidal potential and highlighted the value of prodigiosin as a new candidate to fight Chagas disease.

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“…without bicarbonate (Sigma-Aldrich, São Paulo, Brazil). Conidial suspensions were obtained from sporulated Aspergillus cultures and adjusted to contain 2-5×10 6 conidia/mL by counting in a hemocytometer. To obtain a final concentration of 2-5×10 5 conidia/mL, 1:10 dilutions were prepared in sterile distilled water.…”

mentioning

confidence: 99%

In Vitro Activity of Prodigiosin Isolated From Serratia Marcescens in Combination With Polyenes and Azoles Against Aspergillus Niger

Ivanchenko

2020

DO DESENVOLVIMENTO MUNDIAL COMO RESULTADO DE REALIZAÇÕES EM CIÊNCIA E INVESTIGAÇÃO CIENTÍFICA - Volume 3

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Biopigment are natural compounds (secondary metabolite) produced by many organisms represent one of the important sources of potential lead compounds. Prodigiosin (5[(3-methoxy-5-pyrrol-2-ylidene-pyrrol-2-ylidene) is a red, tripyrrole, water insoluble, bioactive pigment produced by number of different bacteria, actinomycetes and some fungi [1]. Notwithstanding the scarce knowledge of its mechanism of action, prodigiosin appears as a pluripotent molecule, with various health-related properties; the most important being: an anticancer agent [2,3], an immunosuppressant, an antiprotozoal and an antibacterial agent [2,4,5]. Nevertheless, the antimicrobial properties of prodigiosin have often been questioned, particularly because of the high concentrations required for it to be effective, as these exceed the levels causing toxicity in mammalian cells. For this reason, it has been studied in greater depth for its use in anticancer and immunosuppressive therapy, than as an agent to fight infectious agents [2,3,6]. The rate of emergence of fungicide resistance is greater than the pace of fungicide discovery, and the long registration process for new compounds adds further delays. This situation parallels the situation for antibiotics. Increased research activity is thus needed to develop new antifungal drugs [7]. Therefore, combination therapy is a new option for combating such pathogens. Combinations of antibiotics with non-antibiotic activity-enhancing compounds offer a productive strategy to address the widespread emergence of antibiotic-resistant strains [8]. Materials and methods. As a pigment producer, we used the species S. marcescens, namely the pigment-forming strain, which isolated in the laboratories of the Department of Microbiology, virology, and immunology of Bogomolets National Medical University from the bentonite clays of Kurtsivskyi deposit (Crimea, Ukraine). Aspergillus niger ATCC 704 was evaluated against four antifungal agents that included 2 polyene antimycotics: amphotericin B (AMB) and nystatin (NYS), and 2 azole antifungals: intraconazole (INZ) and fluconazole (FNZ) in combination with prodigiosin pigment (PG), using the method of broth microdilution. The MICs of AMB, ITZ, NYS and FNZ (HiMedia Laboratories, Mumbai, India) were determined following the recommendations of the EUCAST protocol for filamentous fungi [9]. All antifungal drugs (except for FNZ, which solubilized in sterile water) were solubilized in dimethyl sulfoxide (DMSO) and the working solutions were prepared in RPMI 1640 medium with 2% glucose, L-glutamine and a pH indicator but

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Using prodigiosin against some gram-positive and gram-negative bacteria and Trypanosoma cruzi

Cited by 15 publications

References 27 publications

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

Tambjamines and Prodiginines: Biocidal Activity against Trypanosoma cruzi

In Vitro Activity of Prodigiosin Isolated From Serratia Marcescens in Combination With Polyenes and Azoles Against Aspergillus Niger

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