Using polystyrene‐co‐maleic acid for molecularly imprinted membranes prepared in supercritical carbon dioxide

Abstract

Supercritical CO 2 (ScCO 2 ) has been used to prepare molecularly imprinted polymers of poly(styreneco-maleic acid) (PSMA) for targeted uracil (URA). The condition of ScCO 2 was on 16 MPa between 35 and 508C. The resultant imprinted membranes prepared at 35 and 508C bound URA with 9.2 6 0.10 and 12.6 6 0.06 lmol g 21 , respectively. Competitive binding studies were undertaken in binary substrate solution containing each of URA/ DMURA, URA/Thymine, and URA/Cytosine with 2 lM. The URA imprinted membrane showed high separation factor (a) with 17 for both URA/DMURA and URA/Thymine and for URA/Cytosine, a 5 13. Results strongly suggested that the URA imprinted membrane had effective selectivity hydrogen bonding to separately bind in the binary components to the template. Effect of organic solvents on the URA imprinting in ScCO 2 was also studied, in addition to comparison of properties with those obtained in both ScCO 2 and water. Evidence presented that ScCO 2 medium was effective to prepare the URA imprinted membrane. We discussed that ScCO 2 fluid was efficient to fix the shape of URA template into the PSMA membrane through hydrogen bonding.

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

“…The majority of reported molecularly imprinted membrane studies has as an ultimate goal, either process development (in particular for separation and purification) or incorporation with sensors . While efforts to develop and apply molecularly imprinted membranes continue, it remains clear that more work is required to address the mechanisms underlying membrane function.…”

A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil

“…MIPs synthesized for nucleic acid bases using aniline as a monomer have capability to recognize Ura [27]. Ura targeted MIP membranes was also prepared by using polystyrene-co-maleic acid matrix obtained in supercritical CO 2 [28]. Imprinting effect was observed to induce crystallization of Ura when a poly (ANco-MAA) membrane was prepared in supercritical CO 2 using phase inversion imprinting technology.…”

Supercritical CO2 for the Reactive Precipitation of Calcium Carbonate: Uses and Applications to Industrial Processing

“…This work represents a step forward in the development of complex delivery systems using clean technologies. In another work, Kobayashi et al report the preparation of an uracil imprinted membrane of poly(styrene-co-maleic acid), demonstrating the flexibility of the technology for the preparation of different types of substrates [59].…”