Using phosphorus-31 nuclear magnetic resonance spectroscopy to characterize organic phosphorus in environmental samples.

Cade-Menun, Barbara J.; Turner, B. L.; Frossard, Emmanuel; Baldwin, Darren S.

doi:10.1079/9780851998220.0021

Cited by 62 publications

(76 citation statements)

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“…Even when using a pulse angle of 90° and a very high relaxation time (15 s) (Cade-Menun, 2005;McDowell et al, 2006), no P was detected in the polyphosphate and phosphonate categories in our soil samples. Similar results were obtained by Rheinheimer et al (2002) and Gatiboni et al (2005), with soils from the same Brazilian region.…”

Section: P-nmr Spectramentioning

confidence: 65%

“…An amount of 0.3 mL D 2 O was added and the mixture vortex-stirred for 5 min. After standing for 120 min, the supernatant was separated by centrifugation (2,500 rpm for 15 min), filtered through a 0.22 µm membrane and transferred to 5 mm NMR tubes (Cade-Menun, 2005). The 31 P spectra were obtained in a Bruker Advance DPX 400 spectrometer at a frequency of 162 MHz with proton decoupling.…”

Section: P-nmr Analysismentioning

confidence: 99%

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Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Gatiboni

Brunetto

Rheinheimer

et al. 2013

Rev. Bras. Ciênc. Solo

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SUMMARYLong-standing applications of mineral fertilizers or types of organic wastes such as manure can cause phosphorus (P) accumulation and changes in the accumulated P forms in the soil. The objective of this research was to evaluate the forms of P accumulated in soils treated with mineral fertilizer or different types of manure in a long-term experiment. Soil was sampled from the 0-5 cm layer of plots fertilized with five different nutrient sources for nine years: 1) control without fertilizer; 2) mineral fertilizer at recommended rates for local conditions; 3) 5 t ha -1 year -1 of moist poultry litter; 4) 60 m 3 ha -1 year -1 of liquid cattle manure and 5) 40 m 3 ha -1 year -1 of liquid swine manure. The 31 P-NMR spectra of soil extracts detected the following P compounds: orthophosphate, pyrophosphate, inositol phosphate, glycerophosphate, and DNA. The use of organic or mineral fertilizer over nine years did not change the soil P forms but influenced their concentration. Fertilization with mineral or organic fertilizers stimulated P accumulation in inorganic forms. Highest inositol phosphate levels were observed after fertilization with any kind of manure and highest organic P concentration in glycerophosphate form in after mineral or no fertilization.Index terms: glycerophosphate, manure, phytate, organic phosphorus, organic fertilization.(1) Received for publication on October 26, 2011 and approved on January 22, 2013.

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Section: P-nmr Spectramentioning

confidence: 65%

Section: P-nmr Analysismentioning

confidence: 99%

Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Gatiboni

Brunetto

Rheinheimer

et al. 2013

Rev. Bras. Ciênc. Solo

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“…Phosphorus in solution is normally considered to be orthophosphate (Soluble Reactive Phosphate or SRP) and is taken by different component members of an aquatic ecosystem. Boyd and Musig (1981) demonstrated that planktons in fish ponds absorbed an average of 41% of 0.30 mg/L addition of orthophosphate within 24 hours; however phosphorus that is not absorbed by planktons is rapidly absorbed mud (Hupfer, et al, 2004;Cade-menum, 2005). Pelagic invertebrates not only transform, they can also translocate the recycled phosphorus within the system (Shapiro, 1984).…”

Section: Introductionmentioning

confidence: 99%

Comparative utilization of phosphorus from sedimentary and igneous phosphate rock by major biotic components of aquatic ecosystem

Chakrabarty

2007

Int. J. Environ. Sci. Technol.

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AB STR ACT:Two laboratory experiments were conducted to evaluate the amount of P utilization from two different types of sparingly soluble phosphate rock by aquatic biotic communities. The first type was Mussoorie Phosphate Rock or MPR (sedimentary in origin) and other was Purulia Phosphate Rock or PPR (igneous in origin). The two trials were with eight different treatment combinations. Among various treatments, fish and Chironomid larvae contributed to some extent in increasing the available sediment phosphate content which in turn increased the soluble reactive phosphate (SRP) of overlying water. Concentration of SRP of overlying water decreased in the treatment with zooplanktons. Depletion of SRP of overlying water due to uptake of orthophosphate by Chlorella was also observed. The sedimentary type phosphate rock proved to be more efficient in releasing phosphate than igneous one.

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“…[10] At the other end of the spectrum researchers have used several sophisticated (and expensive) instruments to explore organic P speciation including ultra-high field mass spectrometry, [11] X-ray absorption near edge structure (XANES) spectroscopy [12,13] (which requires a source of synchrotron radiation), and solution and solid state 31 P NMR spectroscopy. [14] Of these more sophisticated approaches, based on the number of publications, solution 31 P NMR spectroscopy appears to have been the most influential.…”

Section: -X)-mentioning

confidence: 99%

Organic phosphorus in the aquatic environment

Baldwin¹

2013

Environ. Chem.

103

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Environmental context. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. This paper discusses the distribution, cycling and ecological significance of five major classes of organic P in the aquatic environment and discusses several principles to guide organic P research into the future.Abstract. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. Unfortunately, in many studies the 'organic' P fraction is operationally defined. However, there are an increasing number of studies where the organic P species have been structurally characterised -in part because of the adoption of 31 P NMR spectroscopic techniques. There are five classes of organic P species that have been specifically identified in the aquatic environment -nucleic acids, other nucleotides, inositol phosphates, phospholipids and phosphonates. This paper explores the identification, quantification, biogeochemical cycling and ecological significance of these organic P compounds. Based on this analysis, the paper then identifies a number of principles which could guide the research of organic P into the future. There is an ongoing need to develop methods for quickly and accurately identifying and quantifying organic P species in the environment. The types of ecosystems in which organic P dynamics are studied needs to be expanded; flowing waters, floodplains and small wetlands are currently all under-represented in the literature. While enzymatic hydrolysis is an important transformation pathway for the breakdown of organic P, more effort needs to be directed towards studying other potential transformation pathways. Similarly effort should be directed to estimating the rates of transformations, not simply reporting on the concentrations. And finally, further work is needed in elucidating other roles of organic P in the environment other than simply a source of P to aquatic organisms.

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Using phosphorus-31 nuclear magnetic resonance spectroscopy to characterize organic phosphorus in environmental samples.

Cited by 62 publications

References 0 publications

Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Comparative utilization of phosphorus from sedimentary and igneous phosphate rock by major biotic components of aquatic ecosystem

Organic phosphorus in the aquatic environment

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