Using Pd-porphyrin phosphorescence and photodynamic oxygen consumption to study oxygen diffusion in cells

Abstract

MLL cells were incubated with Pd-porphyrin and irradiated at 405 nm. The change in Pd-porphyrin phosphorescence intensity was monitored during treatment. A time dependent diffusion model has been developed to fit the measured phosphorescence signals so that the value of the oxygen diffusion coefficient in cells can be calculated