Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications

Hassanzadeh, Halimeh; Matin, Maryam Moghaddam; Naderi‐Meshkin, Hojjat; Bidkhori, Hamid Reza; Mirahmadi, Mahdi; Raeesolmohaddeseen, Mahmood; Sanjar-Moussavi, Nasser; Bahrami, Ahmad Reza

doi:10.1007/s10561-018-9702-5

Cited by 14 publications

(8 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our groups focused on tissue repair for several years and we found tube formation of BM-EPCs sheets was associated with increasing secretion of SDF-1α, EGF and VEGF. VEGF and EGF can acted as paracrine factors to promote BM-EPCs sheets tube forming [15,16], while the effect of SDF-1 was still unknown. Based on the pro-angiogenic effect of SDF-1 [17] and effect characteristics of tube forming from BM-EPCs sheets, we hypothesize BM-EPCs can form tubular structures via the paracrine activity of SDF-1α/CXCR4 axis.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Paracrine Effect Research of Bone Marrow-Derived Endothelial Progenitor Cell Sheet

Xue¹,

Yin²,

Bai³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The release of a wide array of endothelial progenitor cell (EPC) sheet-secreted paracrine factors is central to the mechanism by which these cells contribute to tissue repair. The purpose of this study was to fabricate BM-EPC sheet and conduct preliminary investigation on the paracrine effect of SDF-1ɑ about the role of the stromal cell-derived factor-1α (SDF-1α)/CXCR4 axis in the form of tubular structures from BM-EPCs sheet.Methods: EPCs derived from rat bone marrow (BM-EPC) were identified and isolated by the cell-surface markers CD34/CD133/VE-cadherin/KDR using flow cytometry, as well as by dual affinity for acLDL and UEA-1. Single-cell suspensions were seeded on temperature-responsive cell culture dishes. After 7 days of incubation, the BM-EPC cells were easily harvested as cell sheets, and a series of biochemical experiments were performed in vitro. The expression levels of SDF-1α/CXCR4 axis-associated genes and proteins were examined using RT‑qPCR and western blot analysis, and enzyme-linked immunosorbent assay (ELISA) was applied to determine the concentrations of VEGF, EGF and SDF-1α in the cell culture medium.Results: The BM-EPC cell sheets were successfully harvested. Moreover, BM-EPC cell sheets have superior proliferation and tube formation activity when compare with single cell suspension. When capillary-like tube were formed from EPCs sheets, the releasing of paracrine factors such as VEGF, EGF, and SDF-1ɑ were increasing. To drive the tube formation of BM-EPCs sheets, our mechanism research showed that the activation of PI3K/AKT/eNOS pathway since the phosphorylation of protein CXCR, PI3K, AKT and eNOS were increasing. Conclusion: BM-EPC cell sheets have superior proliferation and tube formation activity that can be used for tissue repair. The strong ability to secrete paracrine factors was be related to the SDF-1α/CXCR4 axis through PI3K/AKT/eNOS pathway.

show abstract

Section: Introductionmentioning

confidence: 99%

Fabrication and Paracrine Effect Research of Bone Marrow-Derived Endothelial Progenitor Cell Sheet

Xue¹,

Yin²,

Bai³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Our groups focused on tissue repair for several years and we found tube formation of BM-EPCs sheets was associated with increasing secretion of SDF-1α, EGF and VEGF. VEGF and EGF can acted as paracrine factors to promote BM-EPCs sheets tube forming (Hassanzadeh et al 2018 ; Ryu et al 2019 ), while the effect of SDF-1α was still unknown. Based on the pro-angiogenic effect of SDF-1α (Spinetti et al 2017 ) and effect characteristics of tube forming from BM-EPCs sheets, we hypothesize the superior paracrine effect of BM-EPCs sheets may be related to SDF-1α/CXCR4 axis.…”

Section: Introductionmentioning

confidence: 99%

Construction and a preliminary study of paracrine effect of bone marrow-derived endothelial progenitor cell sheet

et al. 2021

View full text Add to dashboard Cite

The release of paracrine factors from endothelial progenitor cell (EPC) sheet is a central mechanism of tissue repair. The purpose of this study was to constuct the rat bone marrow derived-endothelial progenitor cell (BM-EPCs) sheet and investigate invest the role of stromal cell-derived factor-1α (SDF-1α)/CXCR4 axis in the biological function of BM-EPCs sheet. BM-EPC cells were identified by the cell-surface markers-CD34/CD133/VE-cadherin/KDR using flow cytometry and dual affinity for acLDL and UEA-1. After 7 days of incubation, the BM-EPC single-cell suspensions were seeded on thermo-sensitive plate to harvest the BM-EPC cell sheets. The expression levels of SDF-1α/CXCR4 axis-associated genes and proteins were examined using RT-qPCR and western blot analysis, and enzyme-linked immunosorbent assay (ELISA) was applied to determine the concentration of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and SDF-1α in the cell culture medium. The BM-EPC cell sheets were successfully harvested. Moreover, BM-EPC cell sheets have superior migration and tube formation activity when compared with single cell suspension. When capillary-like tube were formed from EPCs sheets, the releasing of paracrine factors such as VEGF, EGF and SDF-1α were increased. To reveal the mechanism of tube formation of BM-EPCs sheets, our research showed that the activation of PI3K/AKT/eNOS pathway was involved in the process, because the phosphorylation of CXCR, PI3K, AKT and eNOS were increased. BM-EPC cell sheets have superior paracrine and tube formation activity than the BM-EPC single-cell. The strong ability to secrete paracrine factors was be potentially related to the SDF-1α/CXCR4 axis through PI3K/AKT/eNOS pathway.

show abstract

“…According to the results, keratinocytes treated with MSC‐CM rather than commercial solution expressed stem cell and differentiation markers similarly to those treated with the commercial solution and had similar proliferation and migration abilities. [ 145 ] In another study, VEGF overexpressed MSC‐CM was shown to ameliorate palmitate‐induced diabetic endothelial dysfunction through the PI3K/ protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and p38 mitogen‐activated protein kinase (p38/MAPK) signaling pathway. [ 146 ] In a study on diabetic wound healing, photobiomodulation therapy, and BM‐MSC‐CM were used separately and in combination to treat infected wound models in diabetic rats.…”

Section: Therapeutic Effects Of Msc‐cm On Diabetes and Related Compli...mentioning

confidence: 99%

Therapeutic Potential of Mesenchymal Stem Cell‐Derived Conditioned Medium for Diabetes Mellitus and Related Complications

Isildar,

Ozkan,

Koyuturk

2023

Advanced Therapeutics

View full text Add to dashboard Cite

Diabetes mellitus (DM) is one of the most life‐threatening metabolic disorders, with 9% of the global prevalence, and it was estimated to be rising to 12.2% in 2045. Currently, there is no definitive treatment for DM. Although life‐saving, insulin administration to control blood sugar is not a cure for DM and is insufficient to prevent DM‐related complications such as nephropathy, neuropathy, or retinopathy. For this reason, studies are continuing to develop treatments that will provide β‐cell regeneration while suppressing autoimmunity. Mesenchymal stem cells (MSCs) are multipotent stem cells with a high proliferation capacity, immunosuppression, and immunomodulation ability. MSCs have gained therapeutic importance with these properties besides their differentiation ability. The immunosuppressive and immunomodulatory properties of the cells arise from the soluble and insoluble factors they secrete into the extracellular environment. Therefore, the culture medium where these cells grow has therapeutic value and is named conditioned medium (CM). In this context, CM obtained from MSCs could provide a similar therapeutic effect with fewer safety concerns. Furthermore, preconditioning of MSCs could improve the effectiveness of these cells and associated cellular products. So, this review summarizes the recent advances in MSC‐derived CMs and their therapeutic potential for DM and related complications.This article is protected by copyright. All rights reserved

show abstract

Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications

Cited by 14 publications

References 75 publications

Fabrication and Paracrine Effect Research of Bone Marrow-Derived Endothelial Progenitor Cell Sheet

Fabrication and Paracrine Effect Research of Bone Marrow-Derived Endothelial Progenitor Cell Sheet

Construction and a preliminary study of paracrine effect of bone marrow-derived endothelial progenitor cell sheet

Therapeutic Potential of Mesenchymal Stem Cell‐Derived Conditioned Medium for Diabetes Mellitus and Related Complications

Contact Info

Product

Resources

About