Using orthogonal array to obtain gradient liquid chromatography conditions of enhanced peak intensity to determine geniposide and genipin with electrospray tandem mass spectrometry

Wang, Shau Chun; Liao, Hui Ju; Lee, Wan Chia; Huang, Chih Min; Tsai, Tung Hu

doi:10.1016/j.chroma.2008.10.035

Cited by 35 publications

(22 citation statements)

References 15 publications

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“…Experimental design was carried out by applying Response Surface Methodology (RSM), widely used for process optimization [36][37][38][39][40][41][42]. This methodology allows optimization of analytical methods by carrying out a relatively small number of experiments and with no prior knowledge of interaction forces between analytes and stationary phase [43][44][45].…”

Section: Data Treatment and Experimental Designmentioning

confidence: 99%

Characterization and optimization by experimental design of a liquid chromatographic method for the separation of hydroxylated polychlorinated biphenyls on a polar-embedded stationary phase

Quintanilla-López

Galindo-Iranzo

Gómara

et al. 2010

Journal of Chromatography A

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Section: Data Treatment and Experimental Designmentioning

confidence: 99%

Characterization and optimization by experimental design of a liquid chromatographic method for the separation of hydroxylated polychlorinated biphenyls on a polar-embedded stationary phase

Quintanilla-López

Galindo-Iranzo

Gómara

et al. 2010

Journal of Chromatography A

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“…Previous study shows that geniposide is absorbed and transformed to genipin in the bowel, indicating that genipin may be the major form of geniposide in blood [6]. Genipin has been report to have anti-inflammatory [7], anti-oxidative [8], anti-thrombotic [9] and neuroprotective activities [10].…”

Section: Introductionmentioning

confidence: 99%

Up-Regulation of TIMP-1 by Genipin Inhibits MMP-2 Activities and Suppresses the Metastatic Potential of Human Hepatocellular Carcinoma

et al. 2012

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Aim of the StudyHepatocellular carcinoma is one of the most malignant human cancers with high metastatic potential. The aim of this study is to investigate the anti-metastatic effect of genipin and its underlying mechanism.Experimental ApproachThe anti-metastatic potential of genipin was evaluated by both cell and animal model. Wound healing and invasion chamber assays were introduced to examine the anti-migration and anti-invasion action of genipin in human hepatocellular carcinoma cell HepG2 and MHCC97L; orthotopical implantation model was used for in vivo evaluation. Gelatin Zymography, Immunoblotting, quantitative real-time polymerase chain reaction and ELISA assays were used to study the mechanisms underlying genipin’s anti-metastatic effect.Key ResultsGenipin suppresses the motility and invasiveness of HepG2 and MHCC97L at non-toxic doses, which may be correlated to the inhibition of genipin on MMP-2 activities in the cells. No significant reduced expression of MMP-2 was observed either at mRNA or at protein level. Furthermore, genipin could specifically up-regulate the expression of TIMP-1, the endogenous inhibitor of MMP-2 activities. Silencing of TIMP-1 by RNA interference abolishes genipin’s anti-metastaic effect. Activation of p38 MAPK signaling was observed in genipin-treated cells, which is responsible for the TIMP-1 overexpression and MMP-2 inhibition. Presence of SB202190, the p38 MAPK inhibitor, attenuates the anti-metastatic potential of genipin in hepatocellular carcinoma. Orthotopical implantation model showed that genipin could suppress the intrahepatic metastatic as well as tumor expansion in liver without exhibiting potent toxicity.ConclusionOur findings demonstrated the potential of genipin in suppressing hepatocellular carcinoma metastasis, and p38/TIMP-1/MMP-2 pathway may be involved as the key mechanism of its anti-metastasis effect.

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“…These methods can accurately determine control samples of rat blood dialysates. In another study, Wang et al reported a method for the determination of geniposide and genipin using orthogonal array to obtain LC-MS/MS signals of enhanced peak intensity [179]. The conditions including gradient establishment times, ionization source temperatures and the concentrations of volatile additive ammonium acetate were investigated.…”

Section: Terpenoidsmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

161

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Using orthogonal array to obtain gradient liquid chromatography conditions of enhanced peak intensity to determine geniposide and genipin with electrospray tandem mass spectrometry

Cited by 35 publications

References 15 publications

Characterization and optimization by experimental design of a liquid chromatographic method for the separation of hydroxylated polychlorinated biphenyls on a polar-embedded stationary phase

Characterization and optimization by experimental design of a liquid chromatographic method for the separation of hydroxylated polychlorinated biphenyls on a polar-embedded stationary phase

Up-Regulation of TIMP-1 by Genipin Inhibits MMP-2 Activities and Suppresses the Metastatic Potential of Human Hepatocellular Carcinoma

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

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