Using open‐tubular capillary electrochromatography with part‐coating column for binding constants determination of β₂‐adrenergic receptor with seven drugs

Abstract

An open‐tubular capillary electrochromatography method has been developed for the determination of binding constants between β2‐adrenergic receptor (β2‐AR) and seven drugs. β2‐AR was oriented immobilized onto one part of inner surface of capillary via microwave‐assisted technical synthesis. According to the linear relationship between coating length and the apparent mobility of analyte, the binding constant (Kb) can be obtained by related theories and equations. The order of Kb values between drugs such as adrenaline hydrochloride, norepinephrine bitartrate, and propranolol hydrochloride with β2‐AR is well consistent with that reported in the literature. By the method, Kb values between four extracts of Radix Paeoniae Rubra and β2‐AR were also successfully obtained. Subsequently, computer models were applied to interpret the CEC experiments. And the results proved to be in good agreement with the method. The work, herein, demonstrates the potential of the method in drug‐receptor affinity interactions evaluation and screening of lead compounds from natural sources.

Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography (2019–2022)

“…Analytes without chirality can be separated by hydrazine-linked COF (Tf-DHzOH) [ 49 ], MNP-β-CD-IL [ 7 ], and the mixture of GOs and MoS 2 [ 50 ]. It is worth noting that using β 2 -adrenergic receptor (β 2 -AR) as the coating [ 51 ] can determine the Kb value of four Paeonia lactiflora peony extracts with respect to affinity, which indicates that OT-CEC may be an effective method for predicting drug activity and searching for lead compounds from traditional Chinese medicine (TCM).…”

“…Overall, we provide readers interested in OT-CEC with the most up-to-date overview of this field, and the related new SPs from 2019 to 2022 are listed in Table 1 [ 3 , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] ]. PubMed and Web of Science were used to search the following characters (also their acronyms) alone or in combination: “Open tubular”, “Capillary electrochromatography”, “Chiral separation”, “Stationary phase”, and “Coating” for relevant literature to analyze recent OT-CEC developments.…”

Application progress of immobilized biomembrane in the discovery of active compounds of natural products

“…The classic separation methods have been proven effective, though there are cumbersome steps and certain active ingredients are easy to be missed. Biomacromolecules such as proteins and enzymes have been widely used to screen active substances in NPs (Chen et al, 2018; Liu et al, 2019) because they can be used as ligands to rapidly and specifically bind to related substances in NP extracts. Compared with them, another biomacromolecule, biomembrane, has unique advantages.…”

Sol‐gel technique for the preparation of β‐cyclodextrin gold nanoparticles as chiral stationary phase in open‐tubular capillary electrochromatography

“…Although OTCEC is considered an alternative to packed‐CEC, it suffers from low phase ratio, usually, the recognition occurs only on the capillary surface. In order to circumvent this problem, different options, e.g., porous layers, sol‐gel technique, multilayer and polymer coatings, etc, have been suggested as solutions to enhance the phase ratio of OTC column, and, in turn, increase the interactions between the analyte and the chiral stationary phase (CSPs) …”