Using of microsatellite DNA profiling to identify hatchery-reared seed and assess potential genetic risks associated with large-scale release of swimming crab Portunus trituberculatus in Panjin, China

Genetic structure in the marbled rockfish(Sebastiscus marmoratus)across most of the distribution in the northwestern Pacific

“…Previous research has shown that a population with small effective population size (Ne) will cause genetic diversity to be depleted, will increase random drift and threaten the survival and evolution of the population (Anderson, Cason, & Chavez, ; Frankham, Briscoe, & Ballou, ). Unlike other marine species, such as Fenneropenaeus chinensis and Portunus trituberculatus, which have suffered too much human interference (for example, intensive catching and stock enhancement) in the northwestern Pacific (Liu et al, ; Song, Li, Zhang, & Gao, ), the S. marmoratus population has a large Ne (67.0 ‐∞), low genetic relatedness (<0.01) and a low level of inbreeding (<0.12). Moreover, Marandel et al () reported that negative Ne estimates are commonly occurred due to large population sizes in the marine environment when limited sample sizes were analyzed.…”

Genetic Stock Identification and Adaptability of Hatchery-Reared Black Rockfish, Sebastes schlegelii, Released Into the North Yellow Sea waters

“…All loci were amplified in each individual. The PCR procedure was carried out as previously reported (Liu et al, 2018). In brief, the 15 µL PCR reactions contained 20 ng of genomic DNA, 1.2 µL of MgCl 2 (2.5 mM), 1.6 µL of 10 × PCR buffer, 0.12 µL of Taq polymerase, 1.2 µL of dNTPs (2.5 mM), 1.2 µL of forward primer labeled with either FAM, HEX, or TAMRA (5 µM) and 1.2 µL of unlabeled reverse primer (5 µM).…”

“…Microsatellite markers provide a non-invasive molecular marker for accurate identification of hatchery-reared individuals following release (Fang et al, 2021). Effective parentage determination, based on microsatellite markers, has been reported in finfish, such as brown trout (Salmo trutta) (Kalayci et al, 2020), crustaceans, such as the swimming crab (Portunus trituberculatus) (Liu et al, 2018), and mollusk, such as Pacific abalone (Haliotis discus) (Sekino et al, 2019). Accuracy of parentage determination in S. trutta, using the eight most informative microsatellite markers, obtained an identification accuracy of 96.08%, whilst CPE was estimated to be 99.9% using 15 microsatellite loci (Kalayci et al, 2020).…”

“…The CPE can reach ≥ 99% when using four microsatellite loci in single-parent parentage identification of released Chinese shrimp (Fenneropenaeus chinensis) and RCR was around 2% (Wang et al, 2014). Liu et al (2018) also performed single-parent parentage identification in the enhancement of P. trituberculatus using six microsatellite loci. This yielded a CPE of > 99.97% and the RCR was 7.2%.…”

“…Hence, DNA profiling can be used to identify hatchery-reared seeds to assess economic efficiency. A set of successful identifications have been reported in finfish species, such as red drum (Sciaenops ocellatus) (Karlsson et al, 2008) and silver carp (Hypophthalmichthys molitrix) (Fang et al, 2021), and in crustaceans, such as Chinese shrimp (Fenneropenaeus chinensis) (Wang et al, 2014) and swimming crab (Portunus trituberculatus) (Liu et al, 2018).…”