Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals

Zerzaňová, A.; Žižkovský, Václav; Kučera, Radim; Klimeš, Jiřı́; Jesenský, Ivan; Dohnal, Jiří; Barrón, D.

doi:10.1016/j.jpba.2007.08.010

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…11,14,18 Unlike the gas chromatographic, HPLC, CE and electroanalytical procedures, the instrument is simple and is not of high cost. [11][12][13][14][15][16][17][18][19][20][21][22] In addition, the developed method is highly sensitive, accurate, precise and selective. These advantages encourage the application of the developed method in routine quality control analysis of the biotin.…”

Section: Resultsmentioning

confidence: 99%

“…As some studies suggest, the enzyme biotinidase, which is involved in the in vivo recycling of the vitamin, serves as a carrier protein for the transport of biotin into the cell as well as a biotin-binding protein in serum. 5 Several analytical methods have been developed for the determination in different sample matrices, e.g., UV-visible spectrophotometry, 6 spectrofluorimetry, 7 electrometry 8-10 high performance liquid hromatography (HPLC), [11][12][13][14][15][16] liquid chromatography-mass spectrometry (LC-MS), 17,18 capillary zone electrophoresis, 19,20 enzyme-linked immunosorbent assay (ELISA). 21 In this study, a highly sensitive spectrofluorimetric method was developed to assay biotin in pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

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Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4‐Fluoro‐7‐nitrobenzofurazan

Sevgi

2010

Chin. J. Chem.

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A simple and highly sensitive spectrofluorimetric method was developed for the determination of biotin in pure and dosage form. The method is based on the derivatization of biotin with 4-fluoro-7-nitrobenzofurazan in borate buffer of pH 9.0 to yield a yellow, fluorescent product. The various chemical conditions that affected the reaction were studied. The method was validated for specificity, linearity, precision, accuracy, recovery and robustness. At optimized experimental conditions, a linear relationship between the fluorescence intensity of the concentration of biotin is observed in the range 45-450 ng/mL. Limit of detection and quantification were 0.038 and 0.114 ng/mL, respectively. The percentage mean recovery was 99.96. The proposed procedure was successfully applied to the determination of biotin in its dosage form with mean recovery of 101.23±1.22 for biotin tablets. The results obtained were in good agreement with those obtained by the reference method.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4‐Fluoro‐7‐nitrobenzofurazan

Sevgi

2010

Chin. J. Chem.

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“…As was mentioned above, the combination of high-performance liquid chromatography with coulometric detection (HPLC-CD) is one of the ways to improve the selectivity of AOs quantification in the samples of complex matrix. Coulometric detection is characterized by high sensitivity, low detection limits, lower background currents and less potential interferences, absolute quantification without calibration graph, possibility of trace analysis [80][81][82]. The application of several electrodes in series (up to sixteen coulometric detectors) allows to build a coulometric array working in a screening mode [83].…”

Section: Coulometric Detection Of Antioxidants In Chromatography and Flow Systemsmentioning

confidence: 99%

Analytical Capabilities of Coulometric Sensor Systems in the Antioxidants Analysis

Ziyatdinova

Budnikov

2021

Chemosensors

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The definition of antioxidants (AOs), their classification and properties as well as electrochemical sensor systems for AOs analysis are briefly discussed. The analytical capabilities of coulometric titration with electrogenerated titrants as sensor systems for AOs determination have been considered in detail. The attention focused on the individual AO quantification that was mainly used in the pharmaceutical analysis and estimation of total antioxidant parameters (total antioxidant capacity (TAC), ferric reducing power (FRP) and ceric reducing/antioxidant capacity (CRAC)) allowing the fast screening of the target samples including their quality control. The main advantages of coulometric sensor systems are pointed out. The selective quantification of individual AO in a complex matrix using a combination of chromatography with coulometric or coulometric array detection under potentiostatic mode is discussed. The future development of coulometric sensor systems for AOs analysis is focused on the application of novel coulometric titrants and the application of coulometric detection in flow injection analysis.

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“…Likewise, the majority of the cited methods employed UV detection, either at a single wavelength or multiple wavelengths (i.e., photodiode array detector) to improve performance. However, a few used other types including coulometric (197,223), light scattering (200), and fluorescence (205) detection.…”

Section: Separation-based Methodologymentioning

confidence: 99%

Pharmaceuticals and Related Drugs

Gilpin

Pachla

2009

Anal. Chem.

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Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals

Cited by 12 publications

References 18 publications

Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4‐Fluoro‐7‐nitrobenzofurazan

Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4‐Fluoro‐7‐nitrobenzofurazan

Analytical Capabilities of Coulometric Sensor Systems in the Antioxidants Analysis

Pharmaceuticals and Related Drugs

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