Using nucleolytic toxins as restriction enzymes enables new RNA applications
Ulli Rothweiler,
Sigurd Eidem Gundesø,
Emma Wu Mikalsen
et al.
Abstract:Over the past five decades, DNA restriction enzymes have revolutionized biotechnology. While these enzymes are widely used in DNA research and DNA engineering, the emerging field of RNA and mRNA therapeutics requires sequence-specific RNA endoribonucleases. Here, we describe EcoToxN1, a member of the type III toxin-antitoxin family of sequence-specific RNA endoribonucleases, and its use in RNA and mRNA analysis. This enzyme recognizes a specific pentamer in a single-stranded RNA and cleaves the RNA within this… Show more
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