Using next generation sequencing to tackle non-typhoidal Salmonella infections

Wain, John; Keddy, Karen; Hendriksen, René S.; Rubino, Salvatore

doi:10.3855/jidc.3080

Cited by 25 publications

(19 citation statements)

References 35 publications

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“…In 2000, the European Comission stated that the most frequently reported Salmonella serovars in humans, based upon reports from nine countries (not including Romania), were S. Enteritidis (59.1%), S. Typhimurium (13.0%), S. Hadar (1.8%), S. Virchow (1.7%), S. Infantis (0.9%), S. Agona (0.8%), S. Brandenburg (0.7%), and S. Newport (0.5%) [18]. Also, in sub-Saharan African countries, an increasing number of cases of multidrugresistant (MDR) non-typhoidal Salmonella (NTS) infections have been reported [19].…”

Section: Discussionmentioning

confidence: 99%

First study of Salmonella in meat in Romania

Mihaiu

Lapusan

Tanasuica

et al. 2014

J Infect Dev Ctries

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Introduction: The increasing antimicrobial resistance of Salmonella isolates is of major public health concern, but information regarding these aspects is still lacking in Romania. This study focused on a detailed and accurate investigation concerning prevalence, serotypes, and antimicrobial resistance patterns of Salmonella strains, isolated from pork and chicken meat, collected from all regions of Romania in 2011. Methodology: The research was conducted on 650 samples of chicken and pork meat collected from production units and retail markets located in various regions of Romania. A total of 149 Salmonella isolates were recovered (22.92%), serotyped, confirmed by PCR, and further tested for antimicrobial susceptibility. Results: Thirteen Salmonella serovars were identified; predominant serovars included Infantis, Typhimurium, Derby and Colindale. Multiple resistance was found in 83.22% (n = 124) of the isolates. The isolates were frequently resistant to tetracycline (80.53%), streptomycin (81.21%), sulfamethoxazole (87.25%), nalidixic acid (65.10%), and ciprofloxacin (42.95%). Additionally, a markedly lower resistance rate was observed for ampicillin (20.81%), chloramphenicol (16.78%), and ceftazidime (11.41%). Among 137 resistant Salmonella isolates, 35 different resistance patterns were found. Conclusion: A high prevalence of Salmonella spp. and a relatively high resistance rate to multiple antimicrobials was found. This data indicates that chicken and pork meat could constitute a source of human exposure to multidrug-resistant Salmonella and therefore could be considered a potential vehicle of resistant Salmonella foodborne diseases. Further actions are needed to succesfully implement a national surveillance program for better monitoring of these resistant pathogens.

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Section: Discussionmentioning

confidence: 99%

First study of Salmonella in meat in Romania

Mihaiu

Lapusan

Tanasuica

et al. 2014

J Infect Dev Ctries

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“…However, there is limited data describing the isolation of ST313 from stool and thus it is unknown whether the presence of S. Typhimurium ST313 in invasive diseases is the consequence of the high prevalence of a regionally dominant clone, possibly causing gastroenteritis in a susceptible human population, or due to a more invasive clone which is just one of many strains circulating in the local population [14].…”

Section: Introductionmentioning

confidence: 99%

Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo

Leekitcharoenphon

Friis

Zankari

et al. 2013

J Infect Dev Ctries

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Introduction: Salmonella enterica serovar Typhimurium ST313 is an invasive and phylogenetically distinct lineage present in sub-Saharan Africa. We report the presence of S. Typhimurium ST313 from patients in the Democratic Republic of Congo and Nigeria. Methodology: Eighteen S. Typhimurium ST313 isolates were characterized by antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). Additionally, six of the isolates were characterized by whole genome sequence typing (WGST). The presence of a putative virulence determinant was examined in 177 Salmonella isolates belonging to 57 different serovars. Results: All S. Typhimurium ST313 isolates harbored resistant genes encoded by bla TEM1b , catA1, strA/B, sul1, and dfrA1. Additionally, aac(6')1aa gene was detected. Phylogenetic analyses revealed close genetic relationships among Congolese and Nigerian isolates from both blood and stool. Comparative genomic analyses identified a putative virulence fragment (ST313-TD) unique to S. Typhimurium ST313 and S. Dublin. Conclusion: We showed in a limited number of isolates that S. Typhimurium ST313 is a prevalent sequence-type causing gastrointestinal diseases and septicemia in patients from Nigeria and DRC. We found three distinct phylogenetic clusters based on the origin of isolation suggesting some spatial evolution. Comparative genomics showed an interesting putative virulence fragment (ST313-TD) unique to S. Typhimurium ST313 and invasive S. Dublin.

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“…While non-typhoidal strains usually remain restricted to the gastro-intestinal tract, typhoidal Salmonella serovars then disseminate from the gastro-intestinal tract to mesenteric lymph nodes and colonize systemic sites, like the liver and spleen (Agbor and McCormick 2011;Wain et al 2013). Often host-adapted Salmonella enterica serovars are associated with severe systemic diseases (e.g.…”

Section: Disease Symptoms Pathogenesis and Burden Of Diseasementioning

confidence: 99%

The zoonotic agent Salmonella

Rabsch¹,

Fruth²,

Simon³

et al. 2014

Zoonoses - Infections Affecting Humans and Animals

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Salmonellosis is one of the most common foodborne diseases and is caused by different serovars of the species Salmonella enterica. More than 2600 different Salmonella serovars have been identified to date. Salmonella is an ubiquitous and quite tenacious bacterium that can survive for years in dry environments by transition into a dormant state or biofilm formation. S. enterica serovars are often associated with animal reservoirs and outbreak investigations reveal that infections in humans constantly result from animal to human transmission. The food vehicles most commonly involved are chicken and poultry, pork, beef, eggs, and seafood.

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Using next generation sequencing to tackle non-typhoidal Salmonella infections

Cited by 25 publications

References 35 publications

First study of Salmonella in meat in Romania

First study of Salmonella in meat in Romania

Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo

The zoonotic agent Salmonella

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