Using neural networks to determine the contribution of danshensu to its multiple cardiovascular activities in acute myocardial infarction rats

Chen, Yuancheng; Cao, Wanlu; Yuan, Chengfu; Zhang, Lin; Chang, Bo-bo; Yang, Wenliang; Liu, Xiaoquan

doi:10.1016/j.jep.2011.08.069

Cited by 21 publications

(13 citation statements)

References 52 publications

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“…This shows the antilipid peroxidative effect of the combination of paeonol and danshensu against injury caused by free radicals. In this context, previous studies have shown danshensu and paeonol exerted significant GSH-elevating and MDA-scavenging effect in a PK-PD model of MI [41] and in a D-gal-induced cognitive impairment model, respectively [42].…”

Section: Discussionmentioning

confidence: 88%

Cardioprotective Effect of Paeonol and Danshensu Combination on Isoproterenol-Induced Myocardial Injury in Rats

et al. 2012

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BackgroundTraditional Chinese medicinal herbs Cortex Moutan and Radix Salviae Milthiorrhizaeare are prescribed together for their putative cardioprotective effects in clinical practice. However, the rationale of the combined use remains unclear. The present study was designed to investigate the cardioprotective effects of paeonol and danshensu (representative active ingredient of Cortex Moutan and Radix Salviae Milthiorrhizae, respectively) on isoproterenol-induced myocardial infarction in rats and its underlying mechanisms.MethodologyPaeonol (80 mg kg−1) and danshensu (160 mg kg−1) were administered orally to Sprague Dawley rats in individual or in combination for 21 days. At the end of this period, rats were administered isoproterenol (85 mg kg−1) subcutaneously to induce myocardial injury. After induction, rats were anaesthetized with pentobarbital sodium (35 mg kg−1) to record electrocardiogram, then sacrificed and biochemical assays of the heart tissues were performed.Principal FindingsInduction of rats with isoproterenol resulted in a marked (P<0.001) elevation in ST-segment, infarct size, level of serum marker enzymes (CK-MB, LDH, AST and ALT), cTnI, TBARS, protein expression of Bax and Caspase-3 and a significant decrease in the activities of endogenous antioxidants (SOD, CAT, GPx, GR, and GST) and protein expression of Bcl-2. Pretreatment with paeonol and danshensu combination showed a significant (P<0.001) decrease in ST-segment elevation, infarct size, cTnI, TBARS, protein expression of Bax and Caspase-3 and a significant increase in the activities of endogenous antioxidants and protein expression of Bcl-2 and Nrf2 when compared with individual treated groups.Conclusions/SignificanceThis study demonstrates the cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial infarction in rats. The mechanism might be associated with the enhancement of antioxidant defense system through activating of Nrf2 signaling and anti-apoptosis through regulating Bax, Bcl-2 and Caspase-3. It could provide experimental evidence to support the rationality of combinatorial use of traditional Chinese medicine in clinical practice.

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Section: Discussionmentioning

confidence: 88%

Cardioprotective Effect of Paeonol and Danshensu Combination on Isoproterenol-Induced Myocardial Injury in Rats

et al. 2012

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“…Thirtytwo grams of Danshen crude drug per 10 ml of injection was used. In Danshen injection, the phenolic acids including salvianolic acid A and B, danshensu and protocatechuic aldehyde are the major hydrophilic components and pharmacologically active constituents (Zhang et al, 2005;Li et al, 2009;Chen et al, 2011). According to the Chinese Pharmacopoeia, danshensu and protocatechuic aldehyde were quantified for the quality control of preparation in the quality standard of Danshen injection.…”

Section: Test Materialsmentioning

confidence: 99%

Acute and sub-chronic toxicity studies of Danshen injection in Sprague-Dawley rats

Wang

Jin

Zhou

et al. 2012

Journal of Ethnopharmacology

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“…The feasibility of the injection dose was examined in the preliminary experiments. Briefly, doses of 20, 40, 60 and 80 mg/kg were selected for the preliminary experiments according to certain studies (14,15). At 60 mg/kg/day, danshensu exerted the maximum antitumor effect, therefore, this dose was selected for use in the combination study with irradiation, which was diluted in 0.2 ml sterile 0.9% normal saline.…”

Section: Methodsmentioning

confidence: 99%

Danshensu, a major water-soluble component of Salvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice

Cao

Ding

et al. 2016

Oncology Letters

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The molecule 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoic acid (danshensu), a herbal preparation used in traditional Chinese medicine, has been found to possess potential antitumor and anti-angiogenesis effects. The aim of the present study was to investigate the efficacy of the combination of radiation therapy (RT) with danshensu in the treatment of Lewis lung carcinoma (LLC) xenografts, whilst exploring and evaluating the mechanism involved. In total, 8-week old female C57BL/6J mice were randomly assigned into 3 groups to receive: RT, RT + cisplatin and RT + danshensu, respectively, when LLC reached 100–150 mm3. Each group was divided into 7 subgroups according to the different irradiation doses that were administered. Tumor growth curves were created and the sensitization enhancement ratios of the drugs were calculated. The experiment was then repeated, and the 4 groups of tumor-bearing mice were treated with natural saline, danshensu, RT + danshensu and RT, respectively. The mice were sacrificed on day 7, and tumor tissue and blood were collected to determine microvessel density, the expression of proangiogenic factors, and the levels of blood thromboxane B2 and 6-keto-prostaglandin-F1α. Tumor hypoxia was also detected using in vivo fluorescence imaging. With respect to LLC xenografts, treatment with danshensu + RT significantly enhanced the effects of tumor growth inhibition (P<0.05). Furthermore, tumor vasculature was remodeled and microcirculation was improved, which significantly reduced tumor hypoxia (P<0.05). The present study demonstrated that danshensu significantly enhanced the radioresponse of LLC xenografts in mice. The mechanism involved may be associated with the alleviation of tumor cell hypoxia following treatment with danshensu + RT, caused by the improvement of tumor microcirculation and the remodeling of tumor vasculature.

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Using neural networks to determine the contribution of danshensu to its multiple cardiovascular activities in acute myocardial infarction rats

Cited by 21 publications

References 52 publications

Cardioprotective Effect of Paeonol and Danshensu Combination on Isoproterenol-Induced Myocardial Injury in Rats

Cardioprotective Effect of Paeonol and Danshensu Combination on Isoproterenol-Induced Myocardial Injury in Rats

Acute and sub-chronic toxicity studies of Danshen injection in Sprague-Dawley rats

Danshensu, a major water-soluble component of Salvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice

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