Using nanoparticles for<i>in situ</i>vaccination against cancer: mechanisms and immunotherapy benefits

Gorbet, Michael-Joseph; Singh, Akansha; Mao, Chenkai; Fiering, Steven; Ranjan, Ashish

doi:10.1080/02656736.2020.1802519

Cited by 14 publications

(9 citation statements)

References 161 publications

(161 reference statements)

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“…Taken together, our results indicate that PTX-LMNP-mediated combined chemo-MHT therapy stands out as a promising strategy against melanoma. Prospectively, we envisage the elucidation of the mechanisms underlying the cytotoxicity induced by the combination of chemotherapy and MHT [ 82 , 83 , 84 , 85 ]. In vivo experiments will certainly challenge the in vitro results herein reported, although other authors have already corroborated the benefits of chemo-MHT and radiation-MHT combined therapy in pre-clinical assays using analogous drug-heat delivery systems [ 50 , 70 , 86 , 87 , 88 ].…”

Section: Discussionmentioning

confidence: 99%

Paclitaxel-Loaded Lipid-Coated Magnetic Nanoparticles for Dual Chemo-Magnetic Hyperthermia Therapy of Melanoma

et al. 2023

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Melanoma is the most aggressive and metastasis-prone form of skin cancer. Conventional therapies include chemotherapeutic agents, either as small molecules or carried by FDA-approved nanostructures. However, systemic toxicity and side effects still remain as major drawbacks. With the advancement of nanomedicine, new delivery strategies emerge at a regular pace, aiming to overcome these challenges. Stimulus-responsive drug delivery systems might considerably reduce systemic toxicity and side-effects by limiting drug release to the affected area. Herein, we report the development of paclitaxel-loaded lipid-coated manganese ferrite magnetic nanoparticles (PTX-LMNP) as magnetosomes synthetic analogs, envisaging the combined chemo-magnetic hyperthermia treatment of melanoma. PTX-LMNP physicochemical properties were verified, including their shape, size, crystallinity, FTIR spectrum, magnetization profile, and temperature profile under magnetic hyperthermia (MHT). Their diffusion in porcine ear skin (a model for human skin) was investigated after intradermal administration via fluorescence microscopy. Cumulative PTX release kinetics under different temperatures, either preceded or not by MHT, were assessed. Intrinsic cytotoxicity against B16F10 cells was determined via neutral red uptake assay after 48 h of incubation (long-term assay), as well as B16F10 cells viability after 1 h of incubation (short-term assay), followed by MHT. PTX-LMNP-mediated MHT triggers PTX release, allowing its thermal-modulated local delivery to diseased sites, within short timeframes. Moreover, half-maximal PTX inhibitory concentration (IC50) could be significantly reduced relatively to free PTX (142,500×) and Taxol® (340×). Therefore, the dual chemo-MHT therapy mediated by intratumorally injected PTX-LMNP stands out as a promising alternative to efficiently deliver PTX to melanoma cells, consequently reducing systemic side effects commonly associated with conventional chemotherapies.

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Section: Discussionmentioning

confidence: 99%

Paclitaxel-Loaded Lipid-Coated Magnetic Nanoparticles for Dual Chemo-Magnetic Hyperthermia Therapy of Melanoma

et al. 2023

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“…In this case, the aim is to stimulate the immune system in order to respond against the tumor, generating a more favorable microenvironment. This can include attenuated microorganisms, physical disruptors, recombinant proteins, and also NPs, among others [57]. As has been said, NPs can load different drugs that can activate immune and cancer cells, generating an immune stimulated tumor microenvironment that allows the immune system to attack and kill them.…”

Section: Fig 3 Schematization Of the Possible Uses Of Multifunctional...mentioning

confidence: 99%

Magnetic Nanoparticles for Immune System Related Diseases Treatment

Baudou¹

2023

Magnetic Nanoparticles for Biomedical Applications

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Nanotechnology is a continuously rising field with different and varied purposes. Nanoparticles (NPs) are defined as particles between 1 and 100 nanometers in diameter, but within this classification larger particles can be incorporated up to 500 nm. Magnetic NPs (MNPs) are generally composed with magnetic elements such as nickel, cobalt and iron, and have the ability to be manipulated through a magnetic field. Recently, these have been the subject of numerous studies because their attractive properties for several potential applications. Among them, we can include its use for diagnosis and medical treatment (theragnosis). Due to their properties, MNPs could be used with the aim of developing immunomodulatory therapies and deal with infectious diseases, cancer, allergies, autoimmune diseases, etc. The use of MNPs provides a novel tool to manipulate the immune response towards a profile according to the proposed objectives. That is why this chapter describes the interaction of MNPs with the immune system as well as its possible applications in immunomodulatory therapies.

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“…74 In addition to as adjuvants for vaccines against microorganisms, a wide variety of NPs are being studied for in situ vaccination for cancer immunotherapy; in this case, NPs are applied in recognized tumours as an immunostimulatory reagent with the goal of generating local and systemic antitumor immune response against untreated tumours. A detailed review of NPs studied for in situ vaccination can be found in Gorbet et al 75 All mechanisms of interaction between the different NPs and the immunological system have not yet been described; there is great variability of results, with some studies showing stimulatory effects 76,77 of NPs on cellular immunity while others inhibitory effects. 78 A reason for this variability of results can be found in the different surface characteristics of the NPs that were used.…”

Section: Immunological Effect Of Nanoparticle Thermal Therapiesmentioning

confidence: 99%

“…In addition to as adjuvants for vaccines against microorganisms, a wide variety of NPs are being studied for in situ vaccination for cancer immunotherapy; in this case, NPs are applied in recognized tumours as an immunostimulatory reagent with the goal of generating local and systemic antitumor immune response against untreated tumours. A detailed review of NPs studied for in situ vaccination can be found in Gorbet et al 75 …”

Section: Immunological Effect Of Nanoparticle Thermal Therapiesmentioning

confidence: 99%

Local tumour nanoparticle thermal therapy: A promising immunomodulatory treatment for canine cancer

Castelló

Carvalho

Bakuzis

et al. 2022

Vet Comparative Oncology

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Distinct thermal therapies have been used for cancer therapy. For hyperthermia (HT) treatment the tumour tissue is heated to temperatures between 39 and 45°C, while during ablation (AB) temperatures above 50°C are achieved. HT is commonly used in combination with different treatment modalities, such as radiotherapy and chemotherapy, for better clinical outcomes. In contrast, AB is usually used as a single modality for direct tumour cell killing. Both thermal therapies have been shown to result in cytotoxicity as well as immune response stimulation. Immunogenic responses encompass the innate and adaptive immune systems and involve the activation of macrophages, dendritic cells, natural killer cells and T cells. Several heat technologies are used, but great interest arises from nanotechnology‐based thermal therapies. Spontaneous tumours in dogs can be a model for cancer immunotherapies with several advantages. In addition, veterinary oncology represents a growing market with an important demand for new therapies. In this review, we will focus on nanoparticle‐mediated thermal‐induced immunogenic effects, the beneficial potential of integrating thermal nanomedicine with immunotherapies and the results of published works with thermotherapies for cancer using dogs with spontaneous tumours, highlighting the works that evaluated the effect on the immune system in order to show dogs with spontaneous cancer as a good model for evaluated the immunomodulatory effect of nanoparticle‐mediated thermal therapies.

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Using nanoparticles forin situvaccination against cancer: mechanisms and immunotherapy benefits

Cited by 14 publications

References 161 publications

Paclitaxel-Loaded Lipid-Coated Magnetic Nanoparticles for Dual Chemo-Magnetic Hyperthermia Therapy of Melanoma

Paclitaxel-Loaded Lipid-Coated Magnetic Nanoparticles for Dual Chemo-Magnetic Hyperthermia Therapy of Melanoma

Magnetic Nanoparticles for Immune System Related Diseases Treatment

Local tumour nanoparticle thermal therapy: A promising immunomodulatory treatment for canine cancer

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