Using mini-xylanosomes as a biotechnological tool for sisal fiber deconstruction and enzyme immobilization

Hamann, Pedro Ricardo Vieira; Gomes, Tainah C.; Silva, Luísa de M. B.; Noronha, Eliane Ferreira

doi:10.1007/s13399-021-02137-x

Cited by 1 publication

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While most studies have used E. coli, Pichia pastoris is a valuable alternative. For example, the multimodular C. thermocellum xylanase gene (xynZ) composed of a N-terminal feruloyl esterase module, followed by a xylan-specific CBM family 6 module, a type I dockerin and a GH10 xylanase domain has been expressed in P. pastoris (Hamann et al 2022). They have successfully fused XynZ to the α-factor for protein secretion.…”

Section: Expression Of DC Componentsmentioning

confidence: 99%

Current challenges in designer cellulosome engineering

Lamote

Fonseca

Vanderstraeten

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic activities due to the enhanced enzymesubstrate proximity and inter-enzyme synergies, but technical hurdles in DC engineering have hindered further progress towards industrial application. The challenge in DC engineering lies in the large diversity of possible building blocks and architectures, resulting in a multivariate and immense design space. Simultaneously, the precise DC composition affects many relevant parameters such as activity, stability and manufacturability. Since protein engineers face a lack of high-throughput approaches to explore this vast design space, DC engineering may result in an unsatisfying outcome. This review provides a roadmap to guide researchers through the process of DC engineering. Each step, starting from concept to evaluation, is described and provided with its challenges, along with possible solutions, both for DCs that are assembled in vitro or are displayed on the yeast cell surface. Key points Construction of designer cellulosomes is a multi-step process. Designer cellulosome research deals with multivariate construction challenges. Boosting designer cellulosome efficiency requires exploring a vast design space.

show abstract

Section: Expression Of DC Componentsmentioning

confidence: 99%