Using metabolomics to assess the sub-lethal effects of zinc and boscalid on an estuarine polychaete worm over time

Sinclair, Georgia M.; O’Brien, Allyson L.; Keough, Michael J.; Souza, David P De; Dayalan, Saravanan; Kanojia, Komal; Kouremenos, Konstantinos A.; Tull, Dedreia; Coleman, Rhys A.; Jones, Oliver A.H.; Long, Sara M.

doi:10.1007/s11306-019-1570-x

Cited by 19 publications

(14 citation statements)

References 47 publications

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“…The pathways affected in the current study are consistent with metal and fungicide exposure responses described in previous research [8,10,14]. This indicates that the biochemical responses seen may be common across species and thus have the potential to be indicators of zinc and boscalid exposure generally.…”

Section: Resultssupporting

confidence: 89%

“…At the start of the second week, to simulate a ‘runoff event’ [49], 75 mg/L of boscalid (Filan ® ) was added half a centimeter below the surface of five of the zinc treatments and five of the controls. Exposure concentrations were based on 10% of the acute toxicity concentration (LC 50 ) in sediment to S. aequisetis (Zinc chloride LC50 = 125 mg/L and boscalid LC 50 = 750 mg/L) (Table S1) [8]. The experimental concentrations were higher than the levels of zinc and boscalid detected in wetlands and estuaries around Victoria, Australia, which range between 0.111 and 3.41 mg/L for zinc [10] and between 0.022 and 3.3 mg/L for boscalid [14], to increase the likelihood of a metabolic response.…”

Section: Methodsmentioning

confidence: 99%

“…Metabolites were extracted using a modified Bligh–Dyer method, as described in Sinclair et al [8]. Briefly, annelid tissue samples were first lysed at 6800 rpm (3 × 45 s), using a Precellys bead-mill attached to a Cryolys cooling unit (Bertin Technologies, Aix En Provence, France) that had been pre-chilled with liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

“…They did not detect changes in growth or behavior but observed a change in molecular gene expression profiles. Similarly, Sinclair et al identified metabolomic changes in Simplisetia aequisetis (an estuarine annelid) following exposure to boscalid (a fungicide) and zinc (a heavy metal) within 24 h of a sublethal exposure while not observing any physical changes in outward appearance [8]. Omics data therefore have the potential to provide informative, contaminant-specific biomarkers for environmental assessments, especially if more than one level of data is used.…”

Section: Introductionmentioning

confidence: 99%

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Metabolite Changes in an Estuarine Annelid Following Sublethal Exposure to a Mixture of Zinc and Boscalid

et al. 2019

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Environmental pollutants such as heavy metals and fungicides pose a serious threat to waterways worldwide. Toxicological assessment of such contaminants is usually conducted using single compound exposures, as it is challenging to understand the effect of mixtures on biota using standard ecotoxicological methods; whereas complex chemical mixtures are more probable in ecosystems. This study exposed Simplisetia aequisetis (an estuarine annelid) to sublethal concentrations of a metal (zinc) and a fungicide (boscalid), both singly and as a mixture, for two weeks. Metabolomic analysis via gas and liquid chromatography-mass spectrometry was used to measure the stress response(s) of the organism following exposure. A total of 75 metabolites, including compounds contributing to the tricarboxylic acid cycle, the urea cycle, and a number of other pathways, were identified and quantified. The multiplatform approach identified distinct metabolomic responses to each compound that differed depending on whether the substance was presented singly or as a mixture, indicating a possible antagonistic effect. The study demonstrates that metabolomics is able to elucidate the effects and mode of action of contaminants and can identify possible outcomes faster than standard ecotoxicological endpoints, such as growth and reproduction. Metabolomics therefore has a possible future role in biomonitoring and ecosystem health assessments.

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Section: Resultssupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolite Changes in an Estuarine Annelid Following Sublethal Exposure to a Mixture of Zinc and Boscalid

et al. 2019

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“…Combined microbial indicator groups can be used to monitor biological changes in aquatic ecosystems (Simonin, Voss, Hassett, Rocca, & Bernhardt, 2019). Sinclair et al (2019) studied the effects of zinc and algal toxins on Simplisetia aequisetis by metabolomics. The data showed that the changes in some metabolites of Simplisetia aequisetis can be detected within 24 hr by metabolomics combined with other methods.…”

Section: Application Of Molecular Biology Methods In Environmental Asmentioning

confidence: 99%

Molecular biological methods in environmental engineering

Zhang

et al. 2020

Water Environment Research

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Microbes are sensitive to environmental changes and can respond in a short time. Genomics, proteomics, transcriptomics, metabolomics, and multigroup association are used to characterize the composition, function, and metabolism of microorganisms, and to evaluate the environment according to the changes in microorganisms, which has important reference and guiding significance of environmental monitoring, management, and repair. In this paper, the application of molecular biological methods to study environmental microorganisms in the fields of wastewater treatment, pollution control, soil improvement, and environmental monitoring in 2019 is reviewed.

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Is substrate choice an overlooked variable in ecotoxicology experiments?

Sinclair

Giannantonio

Jones

et al. 2023

Environ Monit Assess

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It is crucial to understand the effects caused by experimental parameters such as temperature, light, and food type on lab and field-based ecotoxicology experiments, as these variables, and combinations thereof, can affect results. The type of substrate used in exposure experiments, however, is generally assumed to have no effect. This may not always be correct. The metabolic changes in the freshwater crustacean, Austrochiltonia subtenuis exposed to copper, using three common substrates, gauze; toilet paper; and cellulose were investigated. Substrate alone did not affect survival, but each substrate elicited a different metabolic response and adult and juvenile amphipods had different substrate preferences. Several classes of metabolites were shown to change in response to different substrates and toxicant. These included disaccharides, monosaccharides, fatty acids, and tricarboxylic acid cycle intermediates. The results illustrate that metabolomic responses can differ in response to experimental factors that were previously thought not to be significant. In fact, our data indicate that substrate should be viewed as an experimental factor as important to control for as more well-known confounders such as temperature or food, thus challenging the current paradigm. Assuming substrate type has no effect on the experiment could potentially lead to errors in contaminant toxicity assessments. We propose that ideal good practise would be that all experimental factors should be evaluated for their potential influence on metabolomic profiles prior to contaminant response experiments being undertaken.

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Using metabolomics to assess the sub-lethal effects of zinc and boscalid on an estuarine polychaete worm over time

Cited by 19 publications

References 47 publications

Metabolite Changes in an Estuarine Annelid Following Sublethal Exposure to a Mixture of Zinc and Boscalid

Metabolite Changes in an Estuarine Annelid Following Sublethal Exposure to a Mixture of Zinc and Boscalid

Molecular biological methods in environmental engineering

Is substrate choice an overlooked variable in ecotoxicology experiments?

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