Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Detect Carbapenem Resistance within 1 to 2.5 Hours

Burckhardt, Irene; Zimmermann, Stefan

doi:10.1128/jcm.00287-11

Cited by 295 publications

(219 citation statements)

References 15 publications

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“…Recently, the carbapenemase activity of Gram-negative bacilli has been detected by matrix-assisted laser desorption ionizationtime of flight (MALDI-TOF) MS (Burckhardt & Zimmermann, 2011;Carvalhaes et al, 2013). This method relies on the modification of the mass spectrum profile of the carbapenem molecule by carbapenemases (Burckhardt & Zimmermann, 2011).…”

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confidence: 99%

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Detection of carbapenemase activity using VITEK MS: interplay of carbapenemase type and period of incubation

Carvalhaes

Silva

Streling

et al. 2015

Journal of Medical Microbiology

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mentioning

confidence: 99%

“…This method relies on the modification of the mass spectrum profile of the carbapenem molecule by carbapenemases (Burckhardt & Zimmermann, 2011). Most studies have evaluated ertapenem hydrolysis by testing the Microflex LT instrument (Bruker Daltonics) (Carvalhaes et al, 2013).…”

mentioning

confidence: 99%

Detection of carbapenemase activity using VITEK MS: interplay of carbapenemase type and period of incubation

Carvalhaes

Silva

Streling

et al. 2015

Journal of Medical Microbiology

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“…; therefore, determining the overall level of protein expression and function is becoming increasingly important in addition to sequence analysis. A few studies (Burckhardt and Zimmermann, 2011;Hrabak et al, 2011) also identified NDM-1, VIM-1, VIM-2, KPC-2, and various types of imipenem enzymes from carbapenem-resistant P. aeruginosa, K. pneumoniae, and E. coli strains by matrix-assisted laser desorption time-of-flight MS within only 1-2.5 h, with a detection sensitivity of 96.67% and specificity of 97.87%. In the present study, the carbapenemase OXA23 was very accurately identified by MS, and was consistent with the results of phenotype and genotype tests.…”

Section: Discussionmentioning

confidence: 99%

LC-MS/MS: a rapid and simple new method for the determination of carbapenem β-lactamases

Shen¹,

Xu²,

She³

2015

Genet. Mol. Res.

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ABSTRACT. We investigated the application of liquid chromatographytandem mass spectrometry (LC-MS/MS) for pan-drug-resistant Acinetobacter baumannii (PDR-AB) with carbapenem resistance. Eight strains were randomly selected from 84 clinical isolates of PDR-AB strains obtained by the Kirby-Bauer and agar dilution methods. An efflux pump inhibition test was used to screen for the efflux pump phenotype. An ethylenediaminetetraacetic acid (EDTA) synergy test was used to screen for the β-lactamase phenotype, and a three-dimensional test was used to detect extended spectrum β-lactamase (ESBL) and ampicillin C, KPC, and carbapenemase. ESBL genes were amplified by polymerase chain reaction and sequenced. Outer membrane proteins were extracted from a sensitive strain and the PDR-AB strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subjected to LC-MS/ MS. Peptide mass fingerprinting data were retrieved, and proteins with differential expression were identified. Results of the efflux pump inhibition tests showed that the minimum inhibitory concentrations for meropenem were decreased in 4 of the 8 strains by at least 25% of the original value. The results of the EDTA synergy test were negative, and the modified Hodge's tests were positive for all strains. PCR and sequencing confirmed that seven, five, and all eight of the PDR-AB strains contained blaOXA-23, blaTEM-1, and KPC-2, respectively. OXA-23 and CsuC proteins were differentially expressed between the drug-resistant and -sensitive strains. Production of blaOXA-23 enzyme and pilus molecular chaperone to guide synthesis of CsuC protein may be involved in the resistance of A. baumannii to carbapenems. LC-MS/ MS provides a quick and easy method for carbapenemase detection.

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“…Basically, the MS follows the enzymatic hydrolysis of the antimicrobial agent. The sensitivity and specificity of this approach is high (97 % and 98 % , respectively) [21] and the results are available in < 3 h [95] . However, this approach must be validated in routine laboratories to consider replacing the conventional techniques (such as cefoxitin disks, or PCR to detect the mecA gene).…”

Section: Detection Of Resistancementioning

confidence: 99%

“…Recently some resistance markers to one or more antimicrobial agents have been detected by MALDI-TOF MS. Reports suggest that MS has the ability to differentiate methicillin-susceptible S. aureus from methicillinresistant S. aureus (MRSA) strains [93,94] , and also detect carbapenem resistance activity based on the detection of degradation of β -lactam antimicrobials [20,21,95] . Basically, the MS follows the enzymatic hydrolysis of the antimicrobial agent.…”

Section: Detection Of Resistancementioning

confidence: 99%

Mass spectrometry: a revolution in clinical microbiology?

Lavigne

Espinal

Dunyach-Rémy

et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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Recently, different bacteriological laboratory interventions that decrease reporting time have been developed. These promising new broad-based techniques have merit, based on their ability to identify rapidly many bacteria, organisms difficult to grow or newly emerging strains, as well as their capacity to track disease transmission. The benefit of rapid reporting of identification and/or resistance of bacteria can greatly impact patient outcomes, with an improvement in the use of antibiotics, in the reduction of the emergence of multidrug resistant bacteria and in mortality rates. Different techniques revolve around mass spectrometry (MS) technology: matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF MS), PCR combined with electrospray ionization-mass spectrometry (PCR/ESI-MS), iPLEX MassArray system and other new evolutions combining different techniques. This report emphasizes the (r)evolution of these technologies in clinical microbiology.

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Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Detect Carbapenem Resistance within 1 to 2.5 Hours

Cited by 295 publications

References 15 publications

Detection of carbapenemase activity using VITEK MS: interplay of carbapenemase type and period of incubation

Detection of carbapenemase activity using VITEK MS: interplay of carbapenemase type and period of incubation

LC-MS/MS: a rapid and simple new method for the determination of carbapenem β-lactamases

Mass spectrometry: a revolution in clinical microbiology?

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