Using lysosomal membrane stability of haemocytes in Ruditapes philippinarum as a biomarker of cellular stress to assess contamination by caffeine, ibuprofen, carbamazepine and novobiocin

Aguirre-Martínez, G.V.; Buratti, Sara; Fabbri, Elena; DelValls, T.À.; Martı́n-Dı́az, M.L.

doi:10.1016/s1001-0742(12)60207-1

Cited by 101 publications

(34 citation statements)

References 73 publications

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“…In this study, lysosome membrane stabilization was used as an indicator of cellular health status of clams according to previous studies (Aguirre-Martínez et al 2013). When there was a sudden food shortage, the decreased retention periods of neutral red in the control clams suggested that food shortage could cause cellular damage to the normal clams.…”

Section: Discussionmentioning

confidence: 98%

“…Previous studies reported that LC 50 values of ammonia nitrogen to R. philippinarum ranged from 0.65 mg/L (168 h) to 239 mg/L (24 h) (Wang 1989;Wang et al 2012). In order to make sure that the exposure concentration of ammonia nitrogen could cause toxicity to the clams without causing mass mortality, three environment-related doses of ammonia nitrogen were used in our first experiment to screen the proper doses by detecting lysosomal destabilization, which was a well-established indicator of cellular damage (UNEP/RAMOGE 1999;Keppler 2007;Aguirre-Martínez et al 2013). In the second experiment, gill tissues of R. philippinarum were used to detect the variations in metabolism, histological structure and apoptosis rates upon ammonia nitrogen exposure, because the gills are regarded as a major tissue to contact with external environment and the first target of external contaminants (David et al 2008;Natalotto et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Gill damage and neurotoxicity of ammonia nitrogen on the clam Ruditapes philippinarum

Cong¹,

Yang

et al. 2017

Ecotoxicology

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Ammonia nitrogen has been a potential menace to aquatic animals along the coastline of China. Presently, the toxicological effects of ammonia nitrogen were mainly concentrated on fishes, while little attention has been paid to molluscs. In this study, the clam Ruditapes philippinarum was used as the target animal to investigate the toxic effects of ammonia nitrogen. Our results showed that ammonia exposure could significantly reduce the integrity of lysosomes in a dose-dependent manner. Metabolite analysis revealed that exposure doses and duration time of ammonia nitrogen could affect the variation profiles of gill metabolites. In detail, branched chain amino acids, glutamate, choline and phosphocholine were significantly decreased after a one-day exposure. Inosine and phenylalanine were found significantly increased and ATP was decreased after a three-day exposure. The changes of metabolites implied that metabolisms of muscle element, neurotransmission and cell apoptosis of gill tissues would be affected by ammonia exposure. Such inferences were supported by the diminished muscle element, decreased concentrations of catecholamines and increased apoptosis rates, respectively. Therefore, we take advantage of metabolomics integrated with conventional biological assays to find out that ammonia exposure could cause lysosome instability, metabolic disturbance, aberrant gill structures and changes to neurotransmitters, and would result in mollusk gill dysfunction in feeding, respiration and immunity.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Gill damage and neurotoxicity of ammonia nitrogen on the clam Ruditapes philippinarum

Cong¹,

Yang

et al. 2017

Ecotoxicology

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“…Recent studies assessing the chronic toxicity of drugs have been showing higher toxicity than the acute tests, revealing that aquatic organisms of different species (e.g. algae, crustaceans, fish) can be impaired even when exposed to environmentally relevant concentrations of drugs (Martin-Diaz et al, 2009;Li et al, 2010;Aguirre-Martínez et al, 2013a;Tsiaka et al, 2013;Chen et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…philippinarum (Adams & Reeve, 1850), commonly known as the Manila clam, was selected to study the ecotoxicological impact of CBZ in the Ria de Aveiro. This species has already been used in previous studies, under laboratory and field conditions, to assess the toxicity of CBZ (Aguirre-Martínez et al, 2013a, 2013bAlmeida et al, 2014) as well as other pharmaceutical drugs (Contardo-Jara et al, 2011;Matozzo et al, 2012a;Antunes et al, 2013;Milan et al, 2013) and to assess organic and metal pollution (Riba et al, 2004;Martín-Díaz et al, 2007;Figueira et al, 2012). All these studies demonstrated the suitability of R. philippinarum as a bioindicator species in marine environment quality assessment.…”

Section: Study Area and Test Organismmentioning

confidence: 93%

“…Moreover, chronic studies performed with environmental concentrations have shown that CBZ can affect multiple oxidative and biochemical pathways in bivalves, with significant deleterious effects (Martin-Diaz et al, 2009;Contardo-Jara et al, 2011;Chen et al, 2014). The impact of CBZ was reported in other species, namely algae (Tsiaka et al, 2013), crustacean (Aguirre-Martínez et al, 2013a) and fish (Gagné et al, 2006a;Li et al, 2009Li et al, , 2010.…”

Section: Introductionmentioning

confidence: 99%

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Chronic toxicity of the antiepileptic carbamazepine on the clam Ruditapes philippinarum

Almeida

Freitas

Calisto

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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The impacts of carbamazepine (CBZ) on aquatic organisms are yet not well investigated. The present study aimed to better understand the chronic effects of environmentally relevant concentrations of CBZ. The experiment was performed by exposing the filter feeding clam Ruditapes philippinarum to 0.00, 0.03, 0.30, 3.00 and 9.00 μg/L, during 28 days. To assess the chronic toxicity of the drug a battery of biomarkers related with health status and oxidative stress was applied. In order to quantify CBZ in the clam's tissues and in water samples ELISA was used. The present study showed three types of responses on the clams after a chronic exposure to CBZ. For control condition and the lower concentrations (0.03 and 0.30 μg/L) a "similar" metabolic state was observed and the most efficient antioxidant status leading to the elimination of reactive oxygen species formed during the metabolism of CBZ. The concentration of 3.00 μg/L seemed to be a "threshold" concentration, beyond which the concentration levels of CBZ began to exert a toxic effect, compromising the activity of biotransformation and antioxidant enzymes, with notorious effects at the highest CBZ concentration (9.00 μg/L). CBZ also seemed to alter the energy-related responses, especially the glycogen and electron system responses, revealing a slowdown in metabolism at the higher exposure concentrations (3.00 and 9.00 μg/L). Overall, the present study demonstrated that the higher CBZ concentrations can lead to the impairment of antioxidant enzymes compromising the neutralization of reactive oxygen species, and thus the ability to cope with oxidative stress.

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The Effects of Contaminants of Emerging Concern on Water Quality

Schoenfuss

2021

Chemometrics and Cheminformatics in Aquatic Toxicology

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Using lysosomal membrane stability of haemocytes in Ruditapes philippinarum as a biomarker of cellular stress to assess contamination by caffeine, ibuprofen, carbamazepine and novobiocin

Cited by 101 publications

References 73 publications

Gill damage and neurotoxicity of ammonia nitrogen on the clam Ruditapes philippinarum

Gill damage and neurotoxicity of ammonia nitrogen on the clam Ruditapes philippinarum

Chronic toxicity of the antiepileptic carbamazepine on the clam Ruditapes philippinarum

The Effects of Contaminants of Emerging Concern on Water Quality

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