Using isotopically-coded hydrogen peroxide as a surface modification reagent for the structural characterization of prion protein aggregates

Serpa, Jason J.; Makepeace, Karl A.T.; Borchers, Tristan H.; Wishart, David S.; Petrotchenko, Evgeniy V.; Borchers, Christoph H.

doi:10.1016/j.jprot.2013.11.020

Cited by 10 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fichet et al () reported use of VH2O2 for prion inactivation. Isotopically coded H 2 O 2 has been studied for its contribution to allowing quantitative comparison of cellular prion protein (PrP(C)) and aggregated oligomeric (PrP(β)) forms of prion protein through surface modification approaches (Serpa et al ). Duerkop et al () dismissed the role of H 2 O 2 in HSA aggregation where the main factors were reported to be cavitation and high shear stress.…”

Section: Vaporized Hydrogen Peroxidementioning

confidence: 99%

Terminal sterilization of medical devices using vaporized hydrogen peroxide: a review of current methods and emerging opportunities

McEvoy¹,

Rowan

2019

J Appl Microbiol

117

View full text Add to dashboard Cite

Medical devices are an important and growing aspect of healthcare provision and are increasing in complexity to meet established and emerging patient needs. Terminal sterilization plays a vital role in the provision of safe medical devices. While terminal sterilization technologies for medical devices include multiple radiation options, ethylene oxide remains the predominant nonthermal gaseous option, sterilizing c. 50% of all manufactured devices. Vaporized hydrogen peroxide (abbreviated VH2O2 by the International Organization for Standardization) is currently deployed for clinical sterilization applications, where its performance characteristics appear aligned to requirements, constituting a viable alternative low-temperature process for terminal processing of medical devices. However, VH2O2 has operational limitations that create technical challenges for industrial-scale adoption. This timely review provides a succinct overview of VH2O2 in gaseous sterilization and addresses its applicability for terminal sterilization of medical devices. It also describes underappreciated factors such as the occurrence of nonlinear microbial inactivation kinetic plots that may dictate a need to develop a new standard approach to validate VH2O2 for terminal sterilization of medical devices.

show abstract

Section: Vaporized Hydrogen Peroxidementioning

confidence: 99%

Terminal sterilization of medical devices using vaporized hydrogen peroxide: a review of current methods and emerging opportunities

McEvoy¹,

Rowan

2019

J Appl Microbiol

117

View full text Add to dashboard Cite

show abstract

“…15 More recently, mass spectrometry was used to study the structure of rPrP and rPrPβ by measuring the difference in the hydrogen peroxide reactivity of methionines present in the two isoforms. 16 Copper-mediated oxidation of the histidine residues in PrP was studied by mass spectrometrybased analysis of rPrP. 17 The reaction products of lysines and reactive oxygen species in hamster PrP Sc were quantitated by mass spectrometry.…”

Section: Mass Spectrometry-based Study Of the Structure Of Prp Scmentioning

confidence: 99%

Applying the tools of chemistry (mass spectrometry and covalent modification by small molecule reagents) to the detection of prions and the study of their structure

Silva

2014

Prion

View full text Add to dashboard Cite

show abstract

“…Approaches combining these MS technologies with chemical methods such as limited proteolysis, chemical surface modification and hydrogen-deuterium exchange, monitor the solvent accessibility of regions of a protein to observe conformational changes [1,27,28]. The enzymatic cleavage, modification, or deuterium exchange at particular amino acids directly correlates to the exposure of that respective region to the solvent and these can be used to determine structural changes [27,28].…”

Section: Purpose Of Structural Proteomics and Its Common Approachesmentioning

confidence: 99%

“…The enzymatic cleavage, modification, or deuterium exchange at particular amino acids directly correlates to the exposure of that respective region to the solvent and these can be used to determine structural changes [27,28]. Chemical cross-linkers form covalent bonds in proteins, which preserve their cellular context and introduce distance constraints to map their structure.…”

Section: Purpose Of Structural Proteomics and Its Common Approachesmentioning

confidence: 99%

Formaldehyde cross-linking and structural proteomics: Bridging the gap

Srinivasa

Ding

Kast

2015

Methods

View full text Add to dashboard Cite

Using isotopically-coded hydrogen peroxide as a surface modification reagent for the structural characterization of prion protein aggregates

Cited by 10 publications

References 35 publications

Terminal sterilization of medical devices using vaporized hydrogen peroxide: a review of current methods and emerging opportunities

Terminal sterilization of medical devices using vaporized hydrogen peroxide: a review of current methods and emerging opportunities

Applying the tools of chemistry (mass spectrometry and covalent modification by small molecule reagents) to the detection of prions and the study of their structure

Formaldehyde cross-linking and structural proteomics: Bridging the gap

Contact Info

Product

Resources

About