Using immunomagnetic technology and other means to facilitate stem cell homing

Payne, Anthony G.

doi:10.1016/j.mehy.2003.12.008

Cited by 6 publications

(1 citation statement)

References 3 publications

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“…The preparation of novel magnetic nanocomposites (NCs)/nanoparticles of improved properties are another important aspect in biotechnology- and biomedicine-related applications. Magnetically driven separations of small biological components and cells, detoxification of undesirable molecules and antigens, magnetic field-guided delivery of drugs (drug targeting) and genes (gene therapy), relaxation and contrast enhancement in noninvasive magnetic resonance imaging (MRI) of tissues, piezoelectric immunosensors, and magnetic fluid hyperthermia for cancer therapy have been recently disclosed in those areas. − …”

Section: Introductionmentioning

confidence: 99%

Magnetically Responsive Carboxylated Magnetite-Polydipyrrole/Polydicarbazole Nanocomposites of Core−Shell Morphology. Preparation, Characterization, and Use in DNA Hybridization

et al. 2005

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Novel bis-heterocyclic mono- and dicarboxylated dipyrrole and dicarbazole monomers have been synthesized in a modular manner. Their oxidative polymerization around magnetite nanosized particles has been investigated and optimized toward new magnetic magnetite-polydipyrrole/polydicarbazole nanocomposites (NCs) of a core-shell morphology. These NCs were thoroughly characterized by FT-IR, TGA (Thermal Gravimetric Analysis), low- and high-resolution TEM/HR-TEM microscopies, and Mössbauer spectroscopy along with magnetization studies. Exploiting the versatile COOH chemistry (activation by water-soluble diimides) introduced by the polymeric shell, DNA hybridization experiments have been conducted onto NC surfaces using an efficient blue-colored HRP-based enzymatic screening biological system. Highly parallel NC-supported DNA hybridization experimentations revealed that these NCs presented an interesting potential for DNA-based diagnostic applications.

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Section: Introductionmentioning

confidence: 99%

Magnetically Responsive Carboxylated Magnetite-Polydipyrrole/Polydicarbazole Nanocomposites of Core−Shell Morphology. Preparation, Characterization, and Use in DNA Hybridization

et al. 2005

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Magnetic Core Conducting Polymer Shell Nanocomposites forDNAAttachment and Hybridization

Lellouche

2007

Nanotechnologies for the Life Sciences

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Electrostatic binding of nanoparticles to mesenchymal stem cells via high molecular weight polyelectrolyte chains

Heng¹,

Cowan²,

Davalian³

et al. 2009

J Tissue Eng Regen Med

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Combining stem cell transplantation with nanoparticle-mediated delivery of drugs and pharmaceuticals is envisioned to be one of the next major developmental steps in regenerative medicine. However, a major challenge would be to keep nanoparticles co-localized with stem cells upon transplantation or transfusion in situ. Since nanoparticles are physically much smaller in size than cells and would not specifically bind to extracellular matrix, it is easier for them to disperse from the transplantation site via the blood circulation. Conjugating nanoparticles directly to the cell membrane can potentially interfere with cellular function by physically obstructing cell surface receptors from interacting with the extracellular matrix, various growth factors and cytokines and other cells. Moreover, drug-loaded nanoparticles may be internalized into the cytoplasm via endocytosis or phagocytosis, which may wreak damage on the cellular machinery, leading to impaired physiological function or cell death. A novel solution may be to utilize high molecular weight polyelectrolyte chains to electrostatically bind nanoparticles to cells. For this purpose, hyaluronan, poly-L-lysine and chitosan are of special interest, because these molecules are generally recognized to be biocompatible for application in various pharmaceutical and surgical products. This study investigated the use of these molecules to bind nanoparticles to mesenchymal stem cells (MSCs), and a novel technique of conjugating half the cell surface with nanoparticles through the use of polyelectrolyte chains was also developed. This would avoid blocking MSC interaction with cytokines, growth factors, extracellular matrix and other cells within the recipient tissue/organ upon delivery in situ.

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Using immunomagnetic technology and other means to facilitate stem cell homing

Cited by 6 publications

References 3 publications

Magnetically Responsive Carboxylated Magnetite-Polydipyrrole/Polydicarbazole Nanocomposites of Core−Shell Morphology. Preparation, Characterization, and Use in DNA Hybridization

Magnetically Responsive Carboxylated Magnetite-Polydipyrrole/Polydicarbazole Nanocomposites of Core−Shell Morphology. Preparation, Characterization, and Use in DNA Hybridization

Magnetic Core Conducting Polymer Shell Nanocomposites forDNAAttachment and Hybridization

Electrostatic binding of nanoparticles to mesenchymal stem cells via high molecular weight polyelectrolyte chains

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