Using immobilization as a quick diagnostic indicator for resistance to phosphine

Athanassiou, Christos G.; Kavallieratos, Nickolas G.; Brabec, Daniel; Agrafioti, Paraskevi; Sakka, Maria Κ.; Campbell, James F.

doi:10.1016/j.jspr.2019.01.004

Cited by 23 publications

(48 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Food and Agriculture Organization (FAO) protocol, as described by the FAO Plant Protection Bulletin (FAO 1975), and modified by Agrafioti et al [ 8 ], was used for the evaluation of the occurrence of phosphine resistance for each population. In brief, twenty adults of each of the tested species and populations were placed in a 1.5 L glass jar and exposed to phosphine concentration of 30 ppm for 20 h. After the termination of the exposure interval, active—i.e., capable of coordinated movement—and immobilized—i.e., not capable of coordinated movement—adults were recorded, as described by Athanassiou et al [ 23 ]. The whole procedure was repeated three times (three replicates), with three sub-replicates each, with new phosphine production on each replicate, as suggested by Agrafioti et al [ 8 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Nitrogen on Phosphine-Susceptible and -Resistant Populations of Stored Product Insects

Sakka

Gatzali²,

Karathanos

et al. 2020

Insects

Self Cite

View full text Add to dashboard Cite

In this study, we evaluated nitrogen treatment on phosphine-resistant field and -susceptible laboratory populations of different stored product beetles. Nine trials were conducted in commercial nitrogen chambers with the O2 level set at 1.0%. Two different temperatures—i.e., 28 and 40 °C—and three exposure intervals—i.e., 2.5, 3 and 9 d—were used in our tests. Adults of the sawtoothed grain beetle, Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae); the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae); and the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae) were used in the trials. The insects were placed in vials with different commodities per species and population, and their mortality was measured after the termination of each trial. Then, the vials were kept in incubator chambers at 25 °C and 65% relative humidity for 65 d to measure progeny production. Complete parental mortality was observed in all cases for O. surinamensis and S. oryzae, but there was some survival for T. castaneum at 28 °C and 3 d of exposure. In general, progeny production was completely (100%) suppressed, with some exceptions for all species and populations. The results indicate that low oxygen is effective for all species tested, regardless of their resistance status to phosphine, and can be further adopted as an alternative method to mitigate resistance in stored product beetles.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of Nitrogen on Phosphine-Susceptible and -Resistant Populations of Stored Product Insects

Sakka

Gatzali²,

Karathanos

et al. 2020

Insects

Self Cite

View full text Add to dashboard Cite

show abstract

“…In detecting and evaluating resistance to phosphine, there are several protocols that have been developed and evaluated 8,12,18–20 . A recent study, Athanassiou et al 19 .…”

Section: Introductionmentioning

confidence: 99%

Scaling recovery of susceptible and resistant stored product insects after short exposures to phosphine by using automated video‐tracking software

Agrafioti

Brabec

Morrison

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND Phosphine‐susceptible or resistant populations of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) adults were exposed to 0 (control), 1000 and 3000 ppm of phosphine for 15 or 90 min, to estimate behavioral and mobility responses after exposure to phosphine. Knockdown of the exposed individuals after exposure was recorded visually. The total distance moved and velocity of movement were assessed immediately after exposure to phosphine, 2 or 24 h later using a camera coupled with automated video tracking software (i.e. Ethovision®). RESULTS For both species tested, the highest percentage of dead adults was noted at the highest concentration (3000 ppm) for both exposure times. For T. castaneum, total distance moved and velocity decreased as the concentration increased for the susceptible population, whereas there was significant variation among individuals in the resistant population. For R. dominica, the distance moved was reduced at the highest concentrations. Individuals of R. dominica moved less than those of T. castaneum and there were significant differences in mobility between susceptible and resistant populations for both species tested. Recovery was much faster in the case of the resistant populations. CONCLUSIONS Changes in movement parameters can be further exploited in assessing the efficacy of different management tactics, such as trapping and sampling. Automated video tracking systems such as Ethovision® can be used to track and record insect behavioral response, providing a more objective measure of insecticide efficacy than visual categorizations. These data shed light on insect mobility and behavioral responses to fumigation treatments in relation to resistance. © 2020 Society of Chemical Industry

show abstract

“…Based on the results above, further elucidating mobility after insecticidal exposure may be used to develop a comprehensive understanding of insecticide efficacy, and predict eventual mortality, as initial changes in mobility patterns may be correlated with mortality [18,19]. Thus, the objectives of this study were to (1) develop an experimental methodology for using Ethovision ® to examine the mobility of stored product beetles after exposure to an insecticide, and (2) to evaluate movement after exposure to an insecticide that is under development that includes deltamethrin, methoprene, along with the synergist, piperonyl butoxide.…”

Section: Introductionmentioning

confidence: 99%

Mobility of Stored Product Beetles after Exposure to a Combination Insecticide Containing Deltamethrin, Methoprene, and a Piperonyl Butoxide Synergist Depends on Species, Concentration, and Exposure Time

Arthur

Athanassiou

Morrison

2020

Insects

Self Cite

View full text Add to dashboard Cite

Adults of Rhyzopertha dominica (F.), the lesser grain borer, Cryptolestes ferrugineus (Stephens), the rusty grain beetle, and Sitophilus oryzae (L.), the rice weevil, were exposed for 1, 24, and 72 h on wheat treated with concentrations of 0% (untreated controls) to 100% of the proposed label rate of an experimental formulation of deltamethrin + Methoprene + piperonyl butoxide synergist. Movement and velocity of movement were assessed after each exposure time using a camera-based monitoring system (Ethovision®). Movement of R. dominica decreased with increasing concentration and exposure time, so that movement had virtually ceased at the 48 and 72 h exposures. Cryptolestes ferrugineus was less susceptible compared to R. dominica, but there was still a general pattern of decreased movement and velocity of movement with increasing concentration and exposure time. Sitophilus oryzae was the least susceptible species, with less differences at the 1 h exposure interval compared to the other two species, but after 24–72 h, the patterns of declining movement and velocity were apparent as the concentration increased. Data were analyzed using curve-fit equations to show the relationship between concentration and exposure time for each species. Results show that the Ethovison system can be used to assess the sub-lethal effects of exposure to grain protectant insecticides and elucidate behavioral variation between different stored product insects.

show abstract

Using immobilization as a quick diagnostic indicator for resistance to phosphine

Cited by 23 publications

References 22 publications

Effect of Nitrogen on Phosphine-Susceptible and -Resistant Populations of Stored Product Insects

Effect of Nitrogen on Phosphine-Susceptible and -Resistant Populations of Stored Product Insects

Scaling recovery of susceptible and resistant stored product insects after short exposures to phosphine by using automated video‐tracking software

Mobility of Stored Product Beetles after Exposure to a Combination Insecticide Containing Deltamethrin, Methoprene, and a Piperonyl Butoxide Synergist Depends on Species, Concentration, and Exposure Time

Contact Info

Product

Resources

About